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Application of astragalus membranaceus extractive for preparing freshwater fish anti-stress agent and preparation method thereof

A technology of extract of astragalus root and freshwater fish, applied in the field of fish farming, can solve the problems of limited utilization and insufficient utilization, and achieve the effects of inhibiting rollover, reducing low-polarity extraction components, and restoring crucian carp feeding.

Pending Publication Date: 2019-07-30
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In my country, Astragalus is rich in resources, but the utilization is not sufficient, such as leftovers, astragalus whiskers, astragalus dregs, and non-authentic Astragalus, which are produced during the processing of Astragalus as a medicine, and the utilization is very limited.

Method used

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  • Application of astragalus membranaceus extractive for preparing freshwater fish anti-stress agent and preparation method thereof
  • Application of astragalus membranaceus extractive for preparing freshwater fish anti-stress agent and preparation method thereof
  • Application of astragalus membranaceus extractive for preparing freshwater fish anti-stress agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Astragalus extract, its preparation method is as follows:

[0044] (1) Dry 1000g of Astragalus membranaceus in a blast drying oven at 50°C to constant weight, pulverize and pass through a 40-mesh sieve to obtain Astragalus membranaceus powder;

[0045] (2) Take 400g of astragalus powder, mix it according to the ratio of astragalus powder and cyclohexane 1:9, stir at 800r / min for 6h, obtain a clear filtrate and residue after filtration, repeat the extraction of the residue twice, and combine the filtrate extracted three times ;

[0046] (3) The filtrate obtained in step (2) is distilled under reduced pressure to constant weight to remove the cyclohexane to obtain the astragalus cyclohexane extract.

Embodiment 2

[0048] Astragalus extract, its preparation method is as follows:

[0049] (1) Dry 1000g of Astragalus membranaceus in a blast drying oven at 50°C to constant weight, pulverize and pass through a 40-mesh sieve to obtain Astragalus membranaceus powder;

[0050] (2) Take 400g of astragalus powder, mix it according to the ratio of astragalus powder and cyclohexane 1:9, stir at 800r / min for 6h, obtain the residue after filtration, and repeat the extraction of the residue twice;

[0051] (3) Mix the cyclohexane extraction residue in step (2) with ethyl acetate at a ratio of 1:9, stir at 800r / min for 6h, then filter the mixture to obtain a clear filtrate and residue, and repeat the extraction of the residue twice , combined the filtrates extracted 3 times;

[0052] (4) Distill the filtrate obtained in step (3) under reduced pressure to a constant weight to remove ethyl acetate to obtain an extract of ethyl acetate astragalus.

Embodiment 3

[0054] Astragalus extract, its preparation method is as follows:

[0055] (1) Dry 1000g of Astragalus membranaceus in a blast drying oven at 50°C to constant weight, pulverize and pass through a 40-mesh sieve to obtain Astragalus membranaceus powder;

[0056] (2) Take 400g of astragalus powder, mix it according to the ratio of astragalus powder and cyclohexane 1:9, stir at 800r / min for 6h, obtain the residue after filtration, and repeat the extraction of the residue twice;

[0057] (3) Mix the cyclohexane extraction residue in step (2) with ethyl acetate at a ratio of 1:9, stir at 800r / min for 6h, then filter the mixture to obtain the residue, and repeat the extraction twice;

[0058] (4) Mix the residue of ethyl acetate in step (3) with ethanol at a ratio of 1:9, stir at 800r / min for 6h, then filter the mixture to obtain a clear filtrate and residue, repeat the extraction of the residue twice, and combine 3 Secondary extracted filtrate;

[0059] (5) The filtrate obtained in...

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Abstract

The invention provides application of an astragalus membranaceus extractive for restraining freshwater fish culture stress, a feed and a preparation method thereof. The preparation method of the astragalus membranaceus extractive comprises the steps of drying and smashing rhizomes of astragalus membranaceus, sequentially adopting cyclohexane and ethyl acetate for extracting astragalus membranaceuspowder, and after filtering, performing reduced pressure distillation on an ethyl acetate filtrate to reach the constant weight to obtain the astragalus membranaceus extractive. The invention furtherprovides the ratio of the astragalus membranaceus extractive in the anti-stress feed and a preparation method of the feed. The astragalus membranaceus extractive is adopted as the freshwater anti-stress agent to be applied into the feed, can restrain the stress reaction caused by high-density copper sulfate and dipterex in freshwater fish culture, improves the freshwater fish anti-stress ability,and lowers the morbidity and death rate, meanwhile, the novel application of astragalus membranaceus is developed, and astragalus membranaceus resource are effectively utilized.

Description

technical field [0001] The invention belongs to the technical field of fish breeding, and specifically relates to the application of an extract of astragalus in preparing an anti-stress agent for freshwater fish and a preparation method thereof. Background technique [0002] High-density intensification is an important feature of current aquaculture. High-density breeding increases the feeding amount, increases the breeding waste in the water body, and deteriorates the water quality, manifested as a decrease in dissolved oxygen in the water, and excessive ammonia nitrogen and nitrite content. It has been reported that these factors can lead to oxidative stress damage in fish, thereby reducing the digestion and absorption of feed by fish, reducing the growth and immune function of fish, and increasing the morbidity and mortality of fish. High density farming also leads to excessive use of chemical disinfectants and pesticides. Copper sulfate is one of the most commonly used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/30A23K10/37A23K10/22A23K20/158A23K20/174A23K20/142A23K20/20
CPCA23K50/80A23K10/30A23K10/37A23K10/22A23K20/158A23K20/174A23K20/142A23K20/30Y02A40/818Y02P60/87
Inventor 李华涛梁琪吴敏董婷婷王均覃川杰谢碧文
Owner NEIJIANG NORMAL UNIV
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