Application of mulberry leaf extract in inhibiting freshwater fish farming stress, feed, and preparation method of feed
A technology of mulberry leaf extract and freshwater fish, which is applied in the field of fish farming, can solve the problems of unreported application and achieve the effects of improving economic benefits, good extraction effect, and reducing morbidity and mortality
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Embodiment 1
[0042] Mulberry leaf extract, its preparation method is as follows:
[0043] (1) 1000g of mulberry leaves are dried to constant weight in a blast drying oven at 50°C, and crushed through a 18-mesh sieve to obtain mulberry leaf powder;
[0044] (2) Take 400g of mulberry leaf powder, mix it according to the ratio of mulberry leaf powder and cyclohexane 1:6g / mL, stir at 600r / min for 6h, and obtain clarified filtrate and filter residue after filtration;
[0045] (3) According to step (2), the filter residue was extracted twice more, and the filtrates extracted three times were combined, and the filtrate was distilled under reduced pressure to constant weight to remove cyclohexane, so as to obtain the mulberry leaf cyclohexane extract.
Embodiment 2
[0047] Mulberry leaf extract, its preparation method is as follows:
[0048] (1) 1000g of mulberry leaves are dried to constant weight in a blast drying oven at 50°C, and crushed through a 18-mesh sieve to obtain mulberry leaf powder;
[0049] (2) Take 400g of mulberry leaf powder, mix according to the ratio of mulberry leaf powder and cyclohexane 1:6g / mL, stir at 600r / min for 6h, obtain the filter residue after filtration, and repeat the extraction of the filter residue twice;
[0050] (3) Mix the filter residue obtained in step (2) with diethyl ether at a ratio of 1:6g / mL, stir at 600r / min for 6h, filter to obtain a clarified filtrate and filter residue, repeat the extraction of the filter residue twice, and combine the filtrate extracted three times;
[0051] (4) The filtrate obtained in step (3) is distilled under reduced pressure to a constant weight to remove the ether to obtain the ether extract of mulberry leaves.
Embodiment 3
[0053] Mulberry leaf extract, its preparation method is as follows:
[0054] (1) 1000g of mulberry leaves are dried to constant weight in a blast drying oven at 50°C, and crushed through a 18-mesh sieve to obtain mulberry leaf powder;
[0055] (2) Take 400g of mulberry leaf powder, mix according to the ratio of mulberry leaf powder and cyclohexane 1:6g / mL, stir at 600r / min for 6h, obtain the filter residue after filtration, and repeat the extraction of the filter residue twice;
[0056] (3) Mix the filter residue obtained in step (2) with diethyl ether at a ratio of 1:6g / mL, stir at 600r / min for 6h, obtain the filter residue after filtration, and repeat the extraction of the filter residue twice;
[0057] (4) Mix the filter residue obtained in step (3) with acetone at a ratio of 1:6g / mL, stir at 600r / min for 6h, then filter the mixture to obtain a clear residue-free filtrate and filter residue, and repeat the extraction of the filter residue 2 times. Merge the filtrates extra...
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