Application of silymarin in preparation of medicine for preventing and/or treating Parkinson's disease

A technology for silymarin and Parkinson's disease, which is applied to the application field of silymarin in the preparation of medicines for preventing and/or treating Parkinson's disease, and can solve the problem that the prevention and control effect has not yet been reported.

Inactive Publication Date: 2021-07-30
葛鹏飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the prevention and treatment effect of silymarin on nervous system diseases, especially Parkinson's disease

Method used

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  • Application of silymarin in preparation of medicine for preventing and/or treating Parkinson's disease
  • Application of silymarin in preparation of medicine for preventing and/or treating Parkinson's disease
  • Application of silymarin in preparation of medicine for preventing and/or treating Parkinson's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Silymarin inhibits the killing effect of rotenone on human SH-SY5Y cells

[0024] Milk thistle (purchased from Chengdu Ruifensi Co., Ltd.), when used, is prepared into a mother solution with DMSO, and administered after dilution.

[0025] Human SH-SY5Y cells (purchased from China Jikai Biotechnology Co., Ltd.), and rotenone (purchased from Sigma Company) were used to make a cell model of "neuron damage caused by Parkinson's disease".

[0026] Human SH-SY5Y cells were inoculated in DMEM medium in 96-well plates, incubated overnight in a carbon dioxide incubator, and incubated with different concentrations of silymarin (2.0mmol / L and 4.0mmol / L) 1 hour in advance, and then After adding rotenone (30 μmol / L), 8 hours later, the cell death rate was detected by LDH (lactate dehydrogenase) release method.

[0027] Experimental results such as figure 1 shown. The results show that: silymarin alone has no killing effect on human SH-SY5Y cells, and rotenone alone has ...

Embodiment 2

[0033] Example 2 Silymarin inhibits DNA double-strand breaks in human SH-SY5Y cells caused by rotenone

[0034] DNA is the carrier of genetic information, and DNA double-strand breaks are the basis for DNA dissolution and cell death. In order to illustrate the protective effect of silymarin on cells, this experiment detected the effect of silymarin on DNA double-strand breaks induced by rotenone.

[0035] (1) Inoculate human SH-SY5Y cells in DMEM medium in a 6cm-diameter petri dish, in a carbon dioxide incubator overnight, and incubate with silymarin (2.0mmol / L and 4.0mmol / L) 1 hour in advance, then add Rotenone (30 μmol / L), the cells were collected 8 hours later. The collected cells were homogenized, differentially centrifuged, cytoplasmic components, mitochondrial components and nuclear components were separated, the protein concentration of each component was determined by BCA method, and Western blotting analysis was performed. The primary antibody used is the marker pro...

Embodiment 3

[0041] Example 3 Silymarin inhibits the endoplasmic reticulum stress response caused by rotenone

[0042] Endoplasmic reticulum stress is one of the important pathological bases leading to Parkinson's disease. Excessive endoplasmic reticulum stress can induce nuclear translocation of CHOP, leading to cell death.

[0043] In order to illustrate the protective effect of silymarin on cells, this experiment detected the effect of silymarin on the endoplasmic reticulum stress response induced by rotenone by Western blot analysis. Human SH-SY5Y cells were inoculated in DMEM medium in a 6cm-diameter petri dish, incubated overnight in a carbon dioxide incubator, and incubated with different concentrations of silymarin (2.0mmol / L and 4.0mmol / L) 1 hour in advance, and then Rotenone (30 μmol / L) was added, and the cells were collected 8 hours later. The collected cells were homogenized, centrifuged at a differential speed, and the cytoplasmic fraction and the nuclear fraction were separa...

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Abstract

The invention discloses application of silymarin in preparation of a medicine for preventing and/or treating Parkinson's disease, and relates to the field of the medicine for preventing and/or treating Parkinson's disease. Experiments prove that silymarin can inhibit the killing effect of rotenone on human SH-SY5Y cells. The silymarin can inhibit DNA double-chain damage caused by rotenone, can inhibit endoplasmic reticulum stress reaction caused by rotenone, and can inhibit protein aggregation caused by rotenone. Further, the experiments prove that silymarin can inhibit the injury effect of oxygen-glucose deprivation (OGD) on neurons, and has the effect of preventing and/or treating neuronal injury and death after cerebral ischemia. The invention develops a new application of silymarin, and provides a new prevention and treatment way and a new prevention and treatment method for preventing and treating the Parkinson's disease, which is a common nervous system disease affecting the life quality of old people.

Description

technical field [0001] The invention relates to the technical field of Parkinson's disease drugs, in particular to the application of silymarin in the preparation of drugs for preventing and / or treating Parkinson's disease. Background technique [0002] Silychristin is a small molecular compound of flavonoid monomers extracted from the fruit of the genus Milk thistle (Milk thistle) of the family Asteraceae. Molecular formula: C 25 h 22 o 10 , molecular weight 482, white granular crystal. CAS number: 33889-69-9. Silychristin has the characteristics of convenient material acquisition and low price. [0003] Parkinson's disease (Parkinson's disease, PD) is a common neurodegenerative disease, more common in the elderly, the average age of onset is about 60 years old, young Parkinson's disease onset under the age of 40 is rare. The prevalence of PD in people over 65 years old in my country is about 1.7%. Clinical manifestations mainly include resting tremor, bradykinesia, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/357A61K36/28A61P25/16A61K131/00
CPCA61K31/357A61K36/28A61P25/16
Inventor 葛鹏飞罗天飞孟繁凯华聪
Owner 葛鹏飞
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