Culture medium to detect Bifidobacterium and quick detection and counting method

Abstract

The invention discloses a culture medium to detect Bifidobacterium and a quick detection and counting method, and relates to the technical field of Bifidobacterium detection. The culture medium hereincomprises a basic medium, and rendering substrate 5-bromo-4-chloro-3-indol-alpha-D-galactoside and / or 5-bromo-4-chloro-3-indol-beta-D-galactoside; alpha-galactosidase generated by metabolism in the Bifidobacterium culture process reacts with the rendering substrate to release indole, so that a blue Bifidobacterium colony is present on the culture medium, or blue halo is present around the Bifidobacterium colony; interfering bacteria cannot display blue color without alpha-galactosidase; the rendering substrate can inhibit other interfering bacteria; the colony can grow quickly after a surfactant is added; the growth of other interfering bacteria can be inhibited by adding antibiotics to the culture medium herein. The culture medium, when used to detect and count Bifidobacterium, allows the Bifidobacterium colonies to be counted conveniently and quickly; the results are significant, detection cost is low, and accuracy is high.

Description

technical field [0001] The invention relates to the technical field of bifidobacteria detection, more specifically, it relates to a culture medium for detecting bifidobacteria and a method for rapid detection and counting. Background technique [0002] Bifidobacterium belongs to the genus Bifidobacterium (Bifidobacterium), which is an important beneficial microorganism. Under normal circumstances, the intestinal microorganisms in the human body will form a relatively balanced state. If you take antibiotics, radiotherapy, chemotherapy, emotional depression, physical Weakness, lack of immunity, etc. will lead to the imbalance of intestinal flora, and some intestinal microorganisms such as Clostridium perfringens will overproliferate in the intestinal tract and produce ammonia, amines, hydrogen sulfide, skatole, indoles, etc. Harmful substances such as indole, nitrite, and bacterial toxins will further affect the health of the body. [0003] As a physiological beneficial bacte...

AI Technical Summary

Problems solved by technology

Anaerobic culture at 36±1°C for 72h±2h, count all the colonies on the plate after culture as the number of bifidobacteria colonies. Inaccurate

Using the above culture medium to detect the total number of bifidobacteria, the colony of bifidobacteria grows large, easy to interpret, and has a good inhibitory effect on Lactobacillus helveticus, and can accurately detect bifidobacteria in bifidobacteria and Lactobacillus combined nutrition content, but Lactobacillus and Bifidobacteria still grow on the culture medium, only by the size of the colony to interpret, requires the experimenter to have rich experience, human judgment of normal abnormal state, with strong subjective differences, the test The culture medium and detection method of bifidobacteria cannot be detected and counted intuitively and conveniently

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