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Application of rotenone in activating activities of ietalurus punetaus nuclear receptor PXR and cytochrome enzyme CYP3A

A channel catfish and cytochrome technology, which is applied in the direction of nuclear receptors, receptors/cell surface antigens/cell surface determinants, enzymes, etc., can solve the problems of lack of research on fish, and achieve shorter residual time, lower residual amount, Risk reduction effect

Inactive Publication Date: 2019-07-19
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research on CYP3A mostly focuses on mammals, and the research on fish is still very lacking

Method used

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  • Application of rotenone in activating activities of ietalurus punetaus nuclear receptor PXR and cytochrome enzyme CYP3A
  • Application of rotenone in activating activities of ietalurus punetaus nuclear receptor PXR and cytochrome enzyme CYP3A
  • Application of rotenone in activating activities of ietalurus punetaus nuclear receptor PXR and cytochrome enzyme CYP3A

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Experimental program
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Effect test

Embodiment 1

[0022] Expression distribution characteristics of PXR and CYP3A genes in channel catfish tissues:

[0023] RNA was extracted from channel catfish liver, kidney, skin, muscle, brain, heart, gills, spleen, anterior field, midgut, and hindgut according to the instructions of Trizol (Invitrogen). After the total RNA extracted from each tissue of channel catfish was treated with DNase I (Takara), the concentration and quality of the extracted RNA (Aλ260 / Aλ280) were detected by a spectrophotometer. Using reverse transcription kit Prime Script TM RT reagent Kit with gDNA Eraser (Perfect Real Time) was used for reverse transcription to synthesize the first strand of cDNA.

[0024] Screening of primers: According to the full-length cDNA sequences of channel catfish PXR and CYP3A genes published in the NCBI GenBank database, specific primers were designed for general PCR and real-time quantification through Primer5.0, Primer quest tool and Sangon Bioengineering online design For PCR ...

Embodiment 2

[0031] Oral irrigation of channel catfish with natural products monomers alopenidin, catechin, delphinidin, cyanidin, ginkgo, rotenone, daidzin, puerarin, silymarin, nobiletin, quercetin, baicalein (both After any one of the available through commercial purchase), the gill and foregut tissues of channel catfish were collected, the expression of channel catfish nuclear receptor PXR and CYP3A was detected, and the natural products with the effect of activating nuclear receptor PXR were screened as ligands, The results showed that rotenone could act as a natural product ligand for activating nuclear receptor PXR, and the activated receptor PXR could regulate the activity of cytochrome CYP3A enzyme.

[0032] The effect of the screened natural product rotenone on accelerating the elimination of chemical drug residues in channel catfish was further verified through experiments, and the channel catfish were randomly divided into an experimental group and a control group: the experimen...

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Abstract

The present invention studies expression levels of a nuclear receptor PXR and a cytochrome enzyme CYP3A in various tissues of ietalurus punetaus and screens target tissues of gills and foreguts respectively for detecting activities of the PXR and CYP3A, a monomer extracted and purified from natural products is used to conduct mouth-filling on the ietalurus punetaus, the gills and foreguts are collected, the expression levels of the nuclear receptor PXR and cytochrome P450 3A enzyme gene are detected, the natural product monomer of rotenone capable of activating a nuclear receptor PXR pathway and regulating the CYP3A enzyme is screened out, an experiment proves that the rotenone can shorten the residual time of enrofloxacin in muscle with skin (edible tissues) of the ietalurus punetaus from12 d to 7 d, and also significantly reduces the residual amount at the same time point, thus the rotenone can be used to accelerate elimination of quinolone chemical residues in the ietalurus punetaus body, and is of great significance for ensuring quality and safety of aquatic products and reducing risks of intake of the aquatic products containing chemical drug residues by the human body.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal pharmacology, and specifically relates to the application of rotenone in activating channel catfish nuclear receptor PXR and cytochrome CYP3A activity. Background technique [0002] Channel catfish (Ietalurus punetaus) is native to North America and belongs to the order Silurfornes and the family Ictaluridae. It was introduced from Hubei in 1984 and began to be cultivated on a large scale in China. Channel catfish is a large freshwater fish that has the advantages of fast growth, wide adaptability, strong disease resistance, high-quality meat and low price, and is deeply loved by consumers. As of 2015, China has become the largest channel catfish breeding country because the annual production of channel catfish is higher than that of the United States. However, with the expansion of breeding scale, the improvement of intensive farming, the degeneration of species, the emergence of drug abu...

Claims

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Application Information

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IPC IPC(8): C07K14/705C12N9/02
CPCC07K14/70567C12N9/0053C12Y109/03001
Inventor 刘永涛王桢月艾晓辉董靖
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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