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Method for obtaining ophiocordyceps sinensis by infecting hirsutella sinensis with host insects of hepialus armoricanus oberthiir larvae

A technology of bat moth larvae and Trichosporum sinensis, applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve the problems of low mortality, shorten infection ossified time, and high larval mortality, and achieve low mortality, The effect of shortening the zombification time of infection

Active Publication Date: 2019-07-02
INNER MONGOLIA FURUI MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the body surface coating and feeding methods take a long time, the infected larvae have a high mortality rate, and the infection rate is low; the existing puncture infection method is prone to excessive wounds on the surface of the worm body and excessive loss of larvae body fluids due to careless operation And the death rate is high
[0009] Therefore, the existing technology cannot simultaneously achieve the effects of low mortality, high infection rate and shortening the ossified time of infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The bat moth used in this example is Thitarodes gonggaensis collected from Sichuan.

[0027] 1. Preparation of Chinese hairy sporozoites

[0028] The medium for cultivating blastospores is PDA medium: each liter contains 20g of glucose, 200g of potato, 5g of peptone, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g and VB 1 0.02g, the rest is H 2 O (natural pH). After accurate molecular identification, the conidia of Trichosporum sinensis were inserted into the above-mentioned PDA medium, cultured in a shaker (120rpm, 9°C) for 60 days, and the blastospores of Trichosphaera sinensis were collected by centrifugation for injection to infect bat moths larva.

[0029] 2. Breeding of bat moth larvae

[0030] The larvae of the bat moth were mixed and fed with carrots, Polygonum villosa and Potentilla velvet in a mass ratio of 1:1:1 to obtain healthy 5th instar bat moth larvae for infection.

[0031] 3. Injection infection and post-injection care

[0032] Inject the 5th instar bat...

Embodiment 2

[0039] The bat moth used in this example is Thitarodes gonggaensis collected from Sichuan.

[0040] 1. Preparation of Chinese hairy sporozoites

[0041] The medium for cultivating blastospores is PDA medium: each liter contains 20g of glucose, 200g of potato, 5g of peptone, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g and VB 1 0.02g, the rest is H 2 O (natural pH). After accurate molecular identification, the conidia of Trichosporum sinensis were inserted into the above-mentioned PDA medium, cultured in a shaker (120rpm, 9°C) for 60 days, and the blastospores of Trichosphaera sinensis were collected by centrifugation for injection to infect bat moths larva.

[0042] 2. Breeding of bat moth larvae

[0043] The larvae of the bat moth were mixed and fed with carrots, Polygonum villosa and Potentilla velvet in a mass ratio of 1:1:1 to obtain healthy 5th instar bat moth larvae for infection.

[0044] 3. Injection infection and post-injection care

[0045] Inject the 5th instar bat m...

Embodiment 3

[0052] The bat moth used in this example is Thitarodes gonggaensis collected from Sichuan.

[0053] 1. Preparation of Chinese hairy sporozoites

[0054] The medium for cultivating blastospores is PDA medium: each liter contains 20g of glucose, 200g of potato, 5g of peptone, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g and VB 1 0.02g, the rest is H 2 O (natural pH). After accurate molecular identification, the conidia of Trichosporum sinensis were inserted into the above-mentioned PDA medium, cultured in a shaker (120rpm, 9°C) for 60 days, and the blastospores of Trichosphaera sinensis were collected by centrifugation for injection to infect bat moths larva.

[0055] 2. Breeding of bat moth larvae

[0056] The larvae of the bat moth were fed with carrots, Polygonum villosa and Potentilla velvet in a mass ratio of 1:1:1 to obtain healthy, infected 2nd instar larvae of the bat moth.

[0057] 3. Injection infection and post-injection care

[0058] The 2nd instar bat moth larvae i...

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Abstract

The invention discloses a method for obtaining ophiocordyceps sinensis by infecting hirsutella sinensis with host insects of hepialus armoricanus oberthiir larvae. According to the method, the three key steps of the spore morphology (blastospore) of the hirsutella sinensis to be injected, an injection method (operation under an aseptic condition) and post-injection care (at a low temperature of 0-6 DEG C) are improved, so that the infected hepialus armoricanus oberthiir larvae can simultaneously achieve the effects of low death rate (less than 30%) and high infection rate (even 100% of funguscarrying rate) and the effect of shortening the infection ossification time (3-5 months), and the artificial ecological tended ophiocordyceps sinensis with the consistent morphology with wild ophiocordyceps sinensis is obtained.

Description

[0001] Technical field: [0002] The invention belongs to the field of artificial cultivation of Cordyceps sinensis, and in particular relates to a method for obtaining Cordyceps sinensis from larvae of host insects bat moths infected by Trichosporum in China. [0003] Background technique: [0004] Cordyceps sinensis is the most distinctive biological resource in my country, belonging to Ascomycota, Sordariomycetes, Hypocreales, Ophiocordycipitaceae, and Ophiocordyceps. In Tibet, Qinghai, Yunnan, Sichuan, Gansu and other snow-capped alpine regions above 3,000 meters above sea level. [0005] Cordyceps sinensis is the larvae of host insects-bat moths infected by the fungus Ophiocordyceps sinensis in China, and makes them ossified. Under suitable conditions, the fungus scorpion grows and forms a composite form of worm (zombie) grass (fungi fruiting body). structure. [0006] Cordyceps sinensis has many functions such as "tonifying the lungs, strengthening the kidneys, benefitin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 曹莉刘桂清吴华韩日畴
Owner INNER MONGOLIA FURUI MEDICAL SCI
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