Chemiluminiscence detection kit for tacrolimus and preparation method of chemiluminiscence detection kit

Abstract

The invention discloses a chemiluminiscence detection kit for tacrolimus and a preparation method of the chemiluminiscence detection kit. The kit comprises magnetic particles coupled with an antigen or an antibody, an acridinium ester marker, tacrolimus calibrator solution, cleaning solution, chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B. The kit providedby the invention employs the magnetic separation chemiluminescence technology as a detection means, and is combined with the acridinium ester labeling technology at the same time. The kit disclosed bythe invention is simple and convenient to operate, mild in reaction condition, stable in luminous value and less influenced by external conditions. Compared with the prior art, the kit is high in sensitivity, is rapid and convenient in detection, is high in accuracy, is good in repeatability, is high in specificity and the like.

Description

technical field [0001] The invention belongs to the technical field of immune detection and analysis, and in particular relates to a tacrolimus chemiluminescent detection kit and a preparation method thereof. Background technique [0002] Tacrolimus (Tacrolimus, FK506) is a 23-membered macrolide antibiotic extracted from fungal fermentation and a calcineurin inhibitor. It is mainly used in organ transplantation such as liver, kidney and small intestine, and inhibits the rejection reaction of the body, which has very important clinical practical value. The mechanism of action of tacrolimus is mainly through inhibiting the transcription of T cell activation-related cytokine genes (IL-2, IL-3, IL-4, IL-5) and the expression of IL-2 and IL-7 receptors to exert immune function. Inhibitory effect, as a new type of immunosuppressant, has the advantages of strong drug efficacy, low dosage, high graft survival rate, and low incidence of acute rejection. The individual absorption an...

AI Technical Summary

Problems solved by technology

Among them, high-performance liquid chromatography can detect the concentration of the original drug of tacrolimus but not the concentration of its metabolites, and the results are accurate and reliable, but the sample pretreatment is cumbersome, time-consuming, and the detection cost is high, and the detection samples are limited. It is not suitable for routine clinical detection; radioimmunoassay has many steps, requires special detection equipment, and will cause great harm to the health of operators; time-resolved fluorescence immunoassay is slow, and the equipment is expensive, and the cost is relatively Higher; fluorescence polarization immunoassay is based on the competitive binding of the analyte and the analyte coupled with fluorescence to the limited receptor binding sites on the specific analyte antibody, but it requires special equipment, and the The required reagents mainly rely on imports, and the cost is extremely expensive; the enzyme-linked immunoassay method has the disadvantages of low sensitivity, difficulty in realizing full automation, and long detection time.