Fluorescent carbon quantum dot based on biomass and preparation method and application thereof
A technology of carbon quantum dots and biomass, applied in fluorescence/phosphorescence, chemical instruments and methods, preparations for in vivo experiments, etc., can solve problems such as insufficient penetration depth, inability to apply light to the field of diagnosis and treatment, and short wavelength, etc., to achieve Wide range of raw material sources, excellent low toxicity, and simple preparation methods
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Embodiment 1
[0041] The preparation method of the fluorescent carbon quantum dot based on biomass comprises the steps:
[0042] Weigh 30 mg of pheophytin powder, add 30 mL of N,N-dimethylformamide, ultrasonically oscillate for 30 minutes, transfer to a microwave digestion reactor, heat at 150°C for 30 minutes under 500W power, and wait for the solution to cool to room temperature , filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed through a dialysis bag with a molecular weight cut-off of 3500 for 48 hours to remove N,N-dimethylformamide to obtain an aqueous solution of carbon quantum dots, which was finally freeze-dried for 36- After 48h, a biomass-based fluorescent carbon quantum dot solid was obtained.
[0043] figure 1 It is a high-resolution transmission electron microscope image of the fluorescent carbon quantum dots corresponding to Example 1, and the results show that the fluorescent carbon quantum dots made of pheophytin are uniformly dispersed spheric...
Embodiment 2
[0047] The preparation method of the fluorescent carbon quantum dot based on biomass comprises the steps:
[0048] Weigh 30mg of arbutin powder, add 30mL N,N-dimethylformamide, ultrasonically oscillate for 30 minutes, then transfer to a microwave digestion reactor, heat at 150°C for 30 minutes under 500W power, and wait for the solution to cool to room temperature , filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed through a dialysis bag with a molecular weight cut-off of 3500 for 48 hours to remove N,N-dimethylformamide to obtain an aqueous solution of carbon quantum dots, which was finally freeze-dried for 36- After 48 hours, a biomass-based fluorescent carbon quantum dot solid was obtained, and the structure and performance of the fluorescent carbon quantum dot were similar to those of Example 1.
Embodiment 3
[0050] The preparation method of the fluorescent carbon quantum dot based on biomass comprises the steps:
[0051] Weigh 30 mg of chestnut peptide powder, add 30 mL of N,N-dimethylformamide, ultrasonically oscillate for 30 minutes, transfer to a microwave digestion reactor, heat at 150°C for 30 minutes under 500W power, and wait for the solution to cool to room temperature. Filter with a filter membrane with a pore size of 0.22 μm, and then dialyze through a dialysis bag with a molecular weight cut-off of 3500 for 48 hours to remove N,N-dimethylformamide to obtain an aqueous solution of carbon quantum dots, which is finally freeze-dried for 36-48 hours by a lyophilizer Finally, the biomass-based fluorescent carbon quantum dot solid was obtained, and the structure and performance of the fluorescent carbon quantum dot were similar to those in Example 1.
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