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Method for preparing erythrothioneine-containing cosmetic stock solution through hericium erinaceus fermentation

A technology of ergothioneine and Hericium Hericium, which is applied in the direction of cosmetics, cosmetic preparations, and methods based on microorganisms. It can solve the problems of low ergothioneine content, active ingredient application, and insufficient preparation of products that meet the requirements of cosmetic specifications, etc. question

Active Publication Date: 2019-06-28
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 1. Hericium erinaceus is mostly used in the food field, but there is no patent report on the application of its fermented or extracted active ingredients in the field of cosmetics
[0011] 2. The existing fermentation and extraction process applied to the ergothioneine fermentation liquid is not enough to prepare products that meet the requirements of cosmetic specifications
[0012] 3. The content of ergothioneine obtained by strain fermentation in existing studies is generally not high

Method used

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  • Method for preparing erythrothioneine-containing cosmetic stock solution through hericium erinaceus fermentation
  • Method for preparing erythrothioneine-containing cosmetic stock solution through hericium erinaceus fermentation
  • Method for preparing erythrothioneine-containing cosmetic stock solution through hericium erinaceus fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] (1) Preserved strains in test tubes were inoculated into 100ml of seed culture medium with bacterial blocks, and cultured at 25°C for 5 days on a shaker at 150rpm;

[0115] The composition of the seed medium is as follows:

[0116] Sucrose 20g / L, bean cake powder 15g / L, sodium sulfate 0.5g / L, disodium hydrogen phosphate 0.5g / L, acetic acid to adjust the pH to 5.0.

[0117] (2) Seed medium was inoculated into 1L fermentation medium, 200rpm shaker, 25°C fermentation culture for 10 days until no residual sugar;

[0118] The composition of the fermentation medium is as follows:

[0119] Glycerin 25g / L, beef extract 20g / L, sodium sulfate 1g / L, disodium hydrogen phosphate 1g / L, zinc chloride 0.005g / L, pyridoxal phosphate 0.001g / L, acetic acid to adjust pH to 5.0, aspartic acid Acid 10mM, Glutamine 5mM, Betaine 10Mm;

[0120] (3) Fermentation liquid is through homogenization 30min, homogenization rotating speed is 3000rpm / min, then through ultrasonic 15min, ultrasonic power...

Embodiment 2

[0122] (1) Inoculate the bacteria block into 200ml seed culture medium for the strains preserved in test tubes, culture at 25°C for 7 days on a shaker at 200rpm;

[0123] The composition of the seed medium is as follows:

[0124] Glucose 25g / L, bean cake powder 15g / L, sodium sulfate 0.5g / L, disodium hydrogen phosphate 0.5g / L, acetic acid to adjust pH to 5.5

[0125] (2) Seed culture medium was inoculated into 2L fermentation medium, shaken at 200 rpm, and fermented at 25°C for 9 days until there was no residual sugar;

[0126] The composition of the fermentation medium is as follows:

[0127] Glycerin 25g / L, beef extract 20g / L, sodium sulfate 0.75g / L, disodium hydrogen phosphate 1g / L, zinc chloride 0.005g / L, pyridoxal phosphate 0.001g / L, acetic acid to adjust pH to 5.5, asparagus Amino acid 8mM, glutamine 5mM, betaine 15Mm;

[0128] (3) fermented liquid is through homogenizing 40min, and homogenizing rotating speed is 3000rpm / min, then through ultrasonic 30min, ultrasonic p...

Embodiment 3

[0130] (1) Inoculate the bacteria block into 500ml seed culture medium for the strains preserved in test tubes, and incubate at 30°C for 8 days on a shaker at 150rpm;

[0131] The composition of the seed medium is as follows:

[0132] Maltose 50g / L, beef extract 35g / L, potassium chloride 0.75g / L, disodium hydrogen phosphate 0.5g / L, lactic acid to adjust the pH to 4.5.

[0133] (2) Seed medium was inoculated into 5L fermentation medium, 100rpm shaker, 30°C fermentation culture for 15 days until no residual sugar;

[0134] The composition of the fermentation medium is as follows:

[0135] Glycerin 50g / L, peptone 30g / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, ferrous chloride 0.0075g / L, vitamin B120.002g / L, acetic acid to adjust pH to 4.5, aspartic acid Acid 10mM, Glutamine 5mM, Betaine 15Mm;

[0136] (3) fermented liquid is through homogenizing 30min, and homogenizing rotating speed is 3000rpm / min, then through ultrasonic 45min, ultrasonic power is...

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Abstract

The invention discloses a method for preparing an erythrothioneine-containing cosmetic stock solution through hericium erinaceus fermentation. The method includes the following steps of firstly, inoculating hericium erinaceus hypha into a liquid seed culture medium to be cultured to obtain seed liquid; secondly, inoculating the seed liquid into a fermentation culture medium for fermentation, and adding erythrothioneine precursor substances for fermentation to the end to obtain fermentation liquid; thirdly, processing the fermentation liquid containing mycelia to obtain an erythrothioneine-containing fermentation stock solution to serve as the erythrothioneine-containing cosmetic stock solution. The stock solution product contains erythrothioneine with the concentration of 300 mg / L or above, has the functions of high oxidization resistance, radiation prevention and inflammation resistance, and can be added as the raw material to toners, lotions, creams and milks and widely applied to the field of cosmetics.

Description

technical field [0001] The invention relates to the field of cosmetic raw materials, in particular to a method for preparing an ergothioneine cosmetic stock solution by fermentation of Hericium Hericium and the fermented stock solution prepared therefrom. Background technique [0002] Hericium erinaceus belongs to Basidiomycota and Hericiumaceae, and is a well-known medicinal and edible fungus. Its fruiting body and mycelium can be used as medicine. It is flat in nature and sweet in taste, has the functions of helping digestion and benefiting the five internal organs, and is rich in nutrition. The medicinal ingredients of Hericium erinaceus mainly include polysaccharides, oligosaccharides, sterols, fatty acids, erinacein and erinacein, etc., which have the functions of protecting the liver and stomach, lowering blood sugar, protecting nerves, enhancing human immunity, anti-cancer, Anti-oxidation and other effects, in addition, Hericium erinaceus is also rich in sulfur, and ...

Claims

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Application Information

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IPC IPC(8): A61K8/9728A61K8/49A61Q19/00A61Q17/04A61Q19/08
CPCA61K8/49A61Q17/04A61Q19/00A61Q19/08A61K8/9728C12P17/10C12N1/145C12R2001/645
Inventor 陆震魏玉洁贾玉倩孙元军石艳丽郭学平栾贻宏
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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