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Endometrial stem cell exocrine concentrated solution gel preparation for treating endometrial injury, preparation method and drug administration method of preparation

A technology of endometrium and gel preparation, which is applied in the field of endometrial stem cell exosome concentrate gel preparation and its preparation, can solve the problems of short action time, inability of cells to fix lesions, limited therapeutic effect, etc., and achieve increased Effects of stability and activity, improvement of local blood supply, improvement of therapeutic effect

Inactive Publication Date: 2019-06-21
天津欣普赛尔生物医药科技有限公司
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the direct intravenous infusion of endometrial stem cells from the umbilical cord has limited therapeutic effect because the cells are difficult to reach the uterus, and the method of directly injecting endometrial stem cells into the uterus cannot fix the cells in the lesion, and the action time is short

Method used

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Examples

Experimental program
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preparation example Construction

[0016] The preparation method of the endometrial stem cell exosome concentrate liquid gel preparation for treating endometrial injury of the present invention comprises the following steps:

[0017] (1) Cultivation of human endometrial stem cells: select human endometrial stem cells with good growth status and negative microbial detection, P2-P8 human endometrial stem cells, according to 3-5*10 4 / cm 2 The initial density is inoculated in cell culture flasks or cell culture dishes, and the culture medium is a culture system containing fetal bovine serum with a volume ratio concentration of 5-15% in the basal medium;

[0018] (2) After 2-4 days of inoculation and culture, the cell fusion rate reaches 80-95%, and the cell growth state is good. Discard all the culture supernatant, and wash the cells 2-4 times with medical compound electrolyte injection, and then add an appropriate amount of compound electrolyte Solution, make the solution height reach 2-3mm, put it into a carbon...

Embodiment example 1

[0031] Patient Zhang, 35 years old, had endometrial thickness less than 8mm after induced abortion and curettage, and was clinically diagnosed as thin endometrium.

[0032] The patient was treated with endometrial exosome gel preparation three times, each time 0.5ml gel preparation was injected into the uterus, and the treatment interval was two weeks. One month after the treatment was completed (10 days after menstruation), the thickness of the endometrium reached 8.4mm, and the symptoms of endometrial injury were relieved.

Embodiment example 2

[0034] Patient Xu, 39 years old, suffered from severe endocrine disorders caused by long-term work pressure. The thickness of the endometrium was less than 8mm, and the clinical diagnosis was thin endometrium.

[0035] The patient was treated with endometrial exosome gel preparation three times, each time 0.5ml gel preparation was injected into the uterus, and the treatment interval was two weeks. One month after the treatment was completed (12 days after menstruation), the thickness of the endometrium reached 9.2mm, and the symptoms of endometrial injury were relieved.

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Abstract

The invention discloses an endometrial stem cell exocrine concentrated solution gel preparation for treating endometrial injury, a preparation method and a drug administration method of the preparation. A method of starving endometrial stem cells is adopted to obtain a human endometrial stem cell exocrine extract in a short time, and the extract is concentrated through an ultracentrifugation method to obtain a human endometrial stem cell exosome with high concentration, wherein the gel for the human endometrial stem cell exosome contains sodium alginate and a compound electrolyte injection solution as stabilizers, a humectant and a transdermal absorption promoter to form stable and uniform gel-like substances which are beneficial to absorption. The preparation can be used for treating endometrial injury.

Description

technical field [0001] The invention relates to a stem cell exosome concentrate liquid gel preparation, in particular to an endometrial stem cell exosome concentrate liquid gel preparation for treating endometrial damage, a preparation method and an administration method thereof. Background technique [0002] The endometrial injury is mainly the injury of the endometrial basal layer, which is mainly related to curettage during pregnancy. Compared with the normal endometrium, the endometrial basal layer during pregnancy is loose and more susceptible to damage. Based on my country's national conditions and the increasing rate of induced abortion, the occurrence of endometrial damage cannot be ignored. The endometrial basal layer is damaged, which may lead to damage or loss of endometrial stem cells; at the same time, local infection and aseptic inflammation of the injured endometrium can destroy the niche microenvironment of stem cells, resulting in regeneration of epithelial ...

Claims

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Application Information

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IPC IPC(8): A61K35/48A61K9/06A61P15/00
Inventor 张冰晶韩洪起鲁振宇牟鑫煜徐悦
Owner 天津欣普赛尔生物医药科技有限公司
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