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Application of a class of compounds in the preparation of anti-picorna virus drugs

A compound and drug technology, applied in the field of medicine, can solve problems such as poor antiviral effect, and achieve the effect of shortening the disease process, strengthening the protective effect, and protecting the nervous system

Active Publication Date: 2022-02-01
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, the antiviral effects of these two drugs in vitro are relatively poor, and they are often used in combination in clinical practice, such as the treatment of HCV (hepatitis C virus) patients.

Method used

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  • Application of a class of compounds in the preparation of anti-picorna virus drugs
  • Application of a class of compounds in the preparation of anti-picorna virus drugs
  • Application of a class of compounds in the preparation of anti-picorna virus drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] Embodiment 1 Drug Gradient Inhibition Experiment

[0127] RD (human malignant embryonic rhabdomyoma cells) cells were divided into 1×10 4 Cells / well were seeded in 96-well tissue culture plates (Corning, USA) and incubated at 37°C, 5% CO 2 Incubate in atmosphere for 3 days until approximately 100% confluency is reached. After forming a monolayer of RD cells at the bottom of the well, add 60 μL of diluted virus stock solution GZ203ACL21 with an appropriate titer to each well of the 96-well plate (2-8 plaques can be formed in the control well). Then place the plate at 37 °C, 5% CO 2 After incubation for 1 hour in the incubator, overlay medium was added directly to each well. Dilute 200 mM drug (dissolved in DMSO) with DMEM medium to 1 mM concentration. Then, 1.6 μL, 3.2 μL, 6.4 μL, 12.8 μL, 16 μL, and 25.6 μL of the compound were directly added to the covering medium of each well (3-6 duplicate wells). at 37°C, 5% CO 2 After culturing in the incubator for 2 days, pl...

Embodiment 2

[0135] Inhibition to EV-A71 virus in embodiment 2RD cells

[0136] RD cells at 1 x 10 5 cells / well were seeded in a 24-well plate. 37°C, CO 2 Incubate in the incubator until the cells cover the bottom of the plate. Set the titer to 1 x 10 4 PFU / ml of EV-A71 virus liquid GZ203AKL21 was added to each well in an amount of 200 μL per well. 37°C, CO 2 Incubate in an incubator for 1 hour, discard the virus solution in the well, wash each well twice with PBS, and then add 1ml of DMEM culture solution to each well. Then the candidate drug with a concentration of 1 mM was added to the wells in the amounts of 0 μL, 20 μL, 40 μL, 60 μL, 80 μL, and 100 μL, and three replicate wells were set for each concentration. 37°C, CO 2 After incubation in the incubator for 24 hours, 50 μL of the supernatant was added to the virus RNA extraction kit to start RNA extraction. A reverse transcription kit was used for RNA to generate cDNA, which was used as a template for quantitative PCR amplifi...

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Abstract

The invention relates to the application of a class of compounds in the preparation of antiviral drugs. This type of compound can effectively inhibit picornaviruses, and it is outstanding that it has a good inhibitory effect on human enterovirus 71 (EV‑A71), and can be used to treat various diseases caused by picornavirus infection, especially for the treatment of Hand, foot and mouth disease is expected to solve the clinical dilemma of non-targeted drugs for hand, foot and mouth disease. In addition, the compounds involved in the present invention are derived from natural extracts and have good safety.

Description

technical field [0001] The invention belongs to the field of medicine and relates to the application of a class of compounds in the preparation of anti-picorna virus drugs. Background technique [0002] Picornaviridae is the smallest group of RNA viruses. Picornaviruses are extremely small circular viruses; 20-30nm in diameter, single-molecule linear single-stranded positive-sense RNA, no envelope, and 20-face symmetrical capsid. Picornaviridae includes nine genera including Foot-and-Mouth Disease Virus, Cardiovirus, Rhinovirus, Hepavirus, and Enterovirus, the most common of which is Enteroviruses. Enteroviruses, also known as enteroviruses, can cause a wide range of diseases, and different types of viruses may have the same symptoms. Typical symptoms include herpetic angina, hand, foot and mouth disease, and aseptic meningitis. Myocarditis, pericarditis, viral meningitis, and limb paralysis syndrome are more serious diseases. [0003] Enteroviruses are pathogens that ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P31/14A61P31/16
CPCY02A50/30
Inventor 杨湘玲尹应先温创宇向南霖林梦梦刘焕亮
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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