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Large-particle-size agarose microspheres and preparation method thereof

An agarose microsphere, large particle size technology, applied in chemical instruments and methods, other chemical processes, alkali metal oxides/hydroxides, etc., can solve spherical deformation, wide particle size distribution range, and emulsification effect It can achieve good pressure resistance, narrow particle size distribution and good balling effect.

Active Publication Date: 2019-06-18
SENHUI MICROSPHERE TECH SUZHOU CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the increase of the particle size of agarose microspheres, problems such as poor emulsification effect, spherical deformation and wide particle size distribution range often occur when prepared by conventional methods.

Method used

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  • Large-particle-size agarose microspheres and preparation method thereof
  • Large-particle-size agarose microspheres and preparation method thereof
  • Large-particle-size agarose microspheres and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation and crosslinking of liquid paraffin system 500 μm 4% agarose microspheres

[0033] Accurately weigh 32g of agarose powder into 800ml of water, put it into a high-temperature sterilizing pot, set the temperature at 105°C for 60min. Accurately weigh 1.56g of Span 60 and 29.68g of Span 85 in 2400ml of liquid paraffin, dissolve at 75°C, and mix well. After the water phase is sterilized by high temperature, it is taken out and added to the oil phase, the temperature is maintained at 75° C., and the rotation speed is adjusted to 105 rpm. After emulsification for 1 hour, cool down and solidify. Monitor the temperature and keep the rate of temperature drop at 2°C / min. After dropping below 10°C, keep the temperature and cure for 1h. After curing, use petroleum ether, 95% ethanol, and pure water to wash repeatedly. Finally stored in 20% ethanol. The particle size distribution figure of this embodiment product is as figure 1 As shown, the light microsco...

Embodiment 2

[0037] Example 2: Preparation and crosslinking of 800 μm 6% agarose microspheres in toluene system

[0038]Accurately weigh 48g of agarose powder into 800ml of water, put it into a high-temperature sterilization pot, set the temperature at 110°C, and set the time for 120min. Accurately weigh 5.2g of Span 60, 90.8g of Span 80 and 2.5g of Tween 80 in 2400ml of toluene, dissolve at 70°C, and mix well. After the water phase is sterilized by high temperature, it is taken out and added to the oil phase, the temperature is maintained at 70° C., and the rotation speed is adjusted to 150 rpm. After emulsification for 1 hour, cool down and solidify, directly cool down in an ice-water bath, keep the temperature, and solidify for 1 hour. After curing, use 95% ethanol and pure water to wash repeatedly. Finally stored in 20% ethanol. The particle size distribution of the product in this example is as follows Figure 6 shown.

[0039] Accurately weigh 150g of the 800μm 6% agarose micros...

Embodiment 3

[0042] Example 3: Preparation and crosslinking of 500 μm 6% agarose microspheres in liquid paraffin system

[0043] Accurately weigh 48g of agarose powder into 800ml of water, put it into a high-temperature sterilizing pot, set the temperature at 115°C for 60min. Accurately weigh 2.4g of Span 60 and 45.60g of Span 85 in 2400ml of liquid paraffin, dissolve at 75°C, and mix well. After the water phase is sterilized by high temperature, it is taken out and added to the oil phase, the temperature is maintained at 75° C., and the rotation speed is adjusted to 150 rpm. After emulsification for 1 hour, cool down and solidify. Monitor the temperature and keep the rate of temperature drop at 2°C / min. After dropping below 10°C, keep the temperature and cure for 1h. After curing, use petroleum ether, 95% ethanol, and pure water to wash repeatedly. Finally stored in 20% ethanol. The particle size distribution of the product in this example is as follows Figure 7 shown.

[0044] Acc...

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Abstract

The invention discloses a preparation method of large-particle-size agarose microspheres, which comprises the steps of providing an agarose solution having the mass concentration of 2-12% as a water phase; providing an organic solvent, in which an emulsifier is dissolved, as an oil phase; dropwise adding the water phase to the oil phase, and stirring to carry out emulsifying; cooling the emulsified mixed liquid for solidifying to obtain the large-particle-size agarose microspheres, wherein the agarose solution is prepared by dissolving at a temperature of 100-150 DEG C for 20-25 min, the emulsifier is one of, or a mixture of more than two of, span 40, span 60, span 80, span 85, tween 20, tween 60 and tween 80, the volume ratio of the water phase to the oil phase is 1:2 to 1:10, stirring speed is 50-1000 rpm, and emulsifying temperature is 50-100 DEG C. The invention also provides large-particle-size agarose microspheres prepared via the preparation method. The large-particle-size agarose microspheres have the particle size range of 400-1000 mu m, and have good pressure resistance, high temperature tolerance and high flow velocity resistance.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a large-diameter agarose microsphere and a preparation method thereof. Background technique [0002] Natural polysaccharide agarose microspheres have the incomparable advantages of other biological materials such as hydrophilicity, porosity, electrical neutrality, and easy derivatization. Chromatographic separation packing materials based on natural polysaccharide agarose are widely used in various In the separation and purification of macromolecules. [0003] The particle size of agarose microspheres currently used is mainly about 90 μm and 30 μm. However, in the fields of blood purification and blood products, there is a wide demand for agarose microspheres with large particle sizes. With the gradual expansion of the market in these fields, the demand for large-size agarose microspheres is becoming more and more urgent. Therefore, it is meaningful to develop a techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/12C08J3/24C08J7/16C08L5/12C08K5/1515B01J20/24B01J20/28B01J20/30
Inventor 梁曙光孙小六王少云徐箭杨维兴
Owner SENHUI MICROSPHERE TECH SUZHOU CO LTD
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