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Lateral flow immune detection system based on centrifugal microfluidic system

An immunodetection, lateral flow technology, applied in the field of microfluidics, can solve the problems of limited volume, limited detection sensitivity, uncontrollable flow rate of reaction liquid, etc., and achieve the effect of improving sensitivity and high detection effect.

Inactive Publication Date: 2019-06-14
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of test strips is limited by their detection sensitivity
On the one hand, the structure of the test strip itself limits the sample volume; on the other hand, the passive liquid driving mode of the test strip also leads to uncontrollable flow rate of the reaction solution during the reaction process.
Both of these limit the detection sensitivity to a certain extent

Method used

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  • Lateral flow immune detection system based on centrifugal microfluidic system
  • Lateral flow immune detection system based on centrifugal microfluidic system
  • Lateral flow immune detection system based on centrifugal microfluidic system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Specifically, Example 1: Centrifugal microfluidic lateral flow immunoassay (condition 1)

[0055] Such as Figure 4 Shown is a schematic diagram of the chip structure in this embodiment. The first functional module consists of a first sample loading chamber 31, a first reaction chamber 32, a first reaction membrane cavity 33, a first waste liquid chamber 34, a first micropipe 35 and a first sample injection hole / vent hole 36. Before the chip is used, the freeze-dried reagent is stored in the first sample adding chamber 31, and then packaged with a single-sided glue. When in use, the solution to be tested and the buffer are mixed into the first sample loading chamber 31, and the chip is fixed on the chip tray 15. When the centrifuge rotates at a high speed, the reaction liquid enters the first reaction chamber 32 through the first micropipe 35 to contact the reaction membrane in the first reaction membrane cavity 33, reacts at 2000 rpm for 15 minutes (condition 1), and the...

Embodiment 2

[0061] Example 2: Centrifugal microfluidic lateral flow immunoassay (condition 2)

[0062] The operation before the reaction liquid contacts the reaction membrane is the same as in Example 1. When the reaction liquid contacts the reaction membrane, the reaction is completed at 1500 rpm for 45 minutes (condition 2), and the detection result is obtained through mobile phone imaging.

[0063] Further, in an embodiment of the present invention, the lateral flow immunoassay chip further includes a second functional module, wherein the second functional module is composed of a second sample chamber, a whole blood cell separation and quantification chamber, and a buffer solution. The sample chamber, the buffer transfer chamber, the first reagent freeze-drying chamber, the second reaction chamber, the second reaction membrane cavity, the second waste liquid chamber, the second micro-pipe and the second vent, are used for whole blood sample Preset sample processing and processing procedures...

Embodiment 3

[0064] Specifically, Example 3: Application of tumor marker detection in whole blood

[0065] Figure 7 It is a schematic diagram of the chip structure in this embodiment. The second functional module consists of a second sample chamber 41, a whole blood cell separation and quantification chamber 42, a buffer sample chamber 43, a buffer transfer chamber 44, a first reagent freeze-drying chamber 45, a second reaction chamber 46, The second reaction membrane cavity 47, the second waste liquid chamber 48, the second micro pipe 49 and the second vent 50 are composed. In use, the whole blood is added to the second sample adding chamber 41, the buffer is added to the buffer sample adding chamber 43, and the chip is fixed on the chip tray. When the centrifuge rotates at a high speed, the whole blood and buffer solution enter the downstream cavity along the microchannel 49a, respectively. During the process of high-speed centrifugation of whole blood, blood cells gradually settle to fu...

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Abstract

The invention discloses a lateral flow immune detection system based on a centrifugal microfluidic system. The centrifugal microfluidic system comprises a centrifugal microfluidic chip, the lateral flow immune detection system comprises a lateral flow immune reaction membrane, a lateral flow immune detection chip, and a centrifugal operating and controlling device, wherein the lateral flow immunereaction membrane is used for communicating an upstream reaction chamber and a downstream reaction chamber of the centrifugal microfluidic chip, and adjusting the flow rate of liquid flowing through the lateral flow immune reaction membrane by adjusting and controlling the centrifugal rotating speed; the lateral flow immune detection chip comprises a sample addition chamber, a reaction chamber, alateral flow reaction membrane pipeline, a liquid waste tank, and a micro-pipeline, and the lateral flow immune detection chip is used for carrying on preset sample treatment of target samples and detecting the treatment process of the target samples; and the centrifugal operating and controlling device is used for carrying on rotating speed adjustment, positioning and imaging of the lateral flowimmune detection chip. The lateral flow immune detection system integrates lateral flow immune detection and a centrifugal microfluidic technology, thus lateral flow immune detection with the adjustable sample volume and the reaction liquid flow rate can be realized, and the higher detection effect is realized.

Description

Technical field [0001] The invention relates to the technical field of microfluidics, in particular to a lateral flow immune detection system based on a centrifugal microfluidic system. Background technique [0002] Lateral flow immunoassay technology is a low-cost, fast-response, and simple-to-operate diagnostic technology. It is widely used in medical and health, food safety, environmental monitoring and other fields. [0003] Traditional lateral flow detection products are mainly lateral flow detection test strips. However, the application of test strips is limited by its detection sensitivity. On the one hand, the structure of the test strip itself limits the sample volume; on the other hand, the passive liquid driving mode of the test strip also causes the uncontrollable flow rate of the reaction liquid during the reaction process. These two limits their detection sensitivity to a certain extent. Summary of the invention [0004] This application is based on the inventor's k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N35/10G01N33/53B01L3/00
Inventor 徐友春沈敏杰陈一奇
Owner TSINGHUA UNIV
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