Three-dimensional fluorescence spectrum peak searching method
A three-dimensional fluorescence and spectrum technology, applied in the field of three-dimensional fluorescence spectrum peak-seeking, can solve the problems of poor effect, low efficiency, and long time-consuming, and achieve the effects of simple operation, fast peak-finding speed, and reduced time-consuming peak-finding
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[0040] Such as Figure 1 to Figure 5 As shown, a three-dimensional fluorescence spectrum peak-finding method, including automatic peak-finding mode and manual peak-finding mode.
[0041] The specific operation process of the manual peak-finding mode of the 3D fluorescence spectrum is as follows, and please refer to the attached Figures 1 to 3 :
[0042] (1) Standardize each element in the three-dimensional fluorescence spectrum data matrix X to obtain a new standardized three-dimensional fluorescence spectrum data matrix X', each element in X' is equal to the element at the same position in X divided by the corresponding excitation wavelength in X ex=355nm, the element whose emission wavelength em=400nm;
[0043] (2) Generate a three-dimensional fluorescence spectrum contour map according to the three-dimensional fluorescence spectrum data matrix;
[0044] (3) The user manually calibrates the peak position at any point in the area where the peak is considered to exist in t...
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