Method for degrading tobacco straw lignin

Abstract

The invention discloses a method for degrading tobacco straw lignin. The method comprises constructing a peroxidase gene expression vector containing a rice GRP signal peptide gene, a GFP gene and anSHP gene and introducing the vector into agrobacterium LBA4404 to construct a mature transgenic tobacco plant; finally using hydrogen peroxide to degrade the lignin in the mature transgenic tobacco plant. The peroxidase gene expression vector constructed can overexpress the endogenous SHP enzyme of the crop straw during the normal growth and make the enzyme fully contact with the cell wall components like the straw lignin accompanying the normal growth and development of the crop. The lignin content in the crop straw is not affected. After the harvest of the crop straw and the stalk harvestingin the maturity stage, the stalk is treated by a hydrogen peroxide solution with the volume fraction of 5%, and the lignin content of the stalk of the transgenic plant can be reduced by 3%-5%. The method has a good application prospect in the degradation of the crop straw lignin.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for degrading tobacco stalk lignin. Background technique [0002] Lignocellulosic resources are abundant renewable energy substances in nature. As the main carrier of biomass energy, its research and application have been widely concerned at home and abroad. Lignin is one of the three main components of the plant cell wall. It surrounds or binds in the cellulose framework, which can enhance the mechanical strength of the plant body, and at the same time facilitate the water transport of the tissue and resist the adverse invasion of the external environment. The complexity of its structure and the stable form in the body make it difficult to be degraded, industrially separated or transformed, and it has become the primary barrier for the resource utilization of lignocellulose. [0003] One of the key technologies to improve the quality of corn and othe...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for degrading tobacco stalk lignin, which solves the problem that in the prior art, the process of degrading lignin by white rot fungi will cause the loss of lignin in the process of crop growth, which limits the use of biological treatment methods in production. promotional app issues

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