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Preparation method and application of total flavonoids from resina draconis

A technology of total flavonoids of dragon's blood and dragon's blood, which is applied in the field of medicine, can solve the problems of easy elution, complex components, toxic and side effects, etc., and achieve the effect of long-lasting drug effect and prevention and treatment of atherosclerosis

Active Publication Date: 2019-05-21
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Dai drug Dracaena has been proven to block Kv1.3 channel to produce effective prevention and treatment of autoimmune encephalitis and rheumatoid arthritis in rats, and the curative effect is long-lasting. However, Dracaena dracaena comes from The crude product of the serum contains various components such as flavonoids, terpenes, phenols, steroids, etc. The composition is complex, and it is easy to cause unpredictable toxic side effects
In addition, dragon's blood flavonoid monomer, dragon's blood, has also been proved to be able to block Kv1.3 channel to produce effective prevention and treatment effects on autoimmune encephalitis and rheumatoid arthritis rats. The blocking of dragon's blood by dragon's blood The disconnection effect is easily washed out, and the drug effect cannot last

Method used

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  • Preparation method and application of total flavonoids from resina draconis
  • Preparation method and application of total flavonoids from resina draconis
  • Preparation method and application of total flavonoids from resina draconis

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Effect test

Embodiment 1

[0027] This embodiment provides a preparation method of total flavonoids of dragon's blood, the specific process is as follows:

[0028] Crush the medicinal material of dragon's blood into a coarse powder, add 11 times the amount of ethanol and ethyl acetate mixed solvent, and reflux extraction at 50-69°C for 2 times, each time for 15-29 minutes; among them, ethanol and ethyl acetate The volume ratio of ester is 5:5~1:10. Preferably, the reflux extraction temperature is 65° C., each reflux extraction time is 25 minutes, and the volume ratio of ethanol to ethyl acetate is 2:8. The content of total flavonoids in the extract was taken as reference substance with loureirin B (Loureirin B, LrB, Shanghai Chunyou Biotechnology Co., Ltd.), and the absorbance of the reference substance and the extract was measured at 277nm by a UV spectrophotometer, and the concentration of the reference substance was taken as the horizontal Coordinates, the absorbance value is the ordinate, draws a s...

Embodiment 2

[0033] In this embodiment, the up-and-down method is used to measure the total flavonoids of dragon's blood and the acute toxicity of dragon's blood. The specific measurement process is as follows: select two groups of female mice, A and B, with five mice in each group, and treat the mice in group A with 2000 mg according to the up-down method. The total flavonoids of dragon's blood was injected intraperitoneally at the dose level of 2000 mg / kg, and the mice in group B were injected with dragon's blood at a dose level of 2000 mg / kg intraperitoneally, and the survival of the mice in the two groups was observed. Among them, the operation method of the upper and lower method, the limit test of the dose level of 2000mg / kg: the test substance is given to a mouse respectively, if the mouse dies, then the main test is carried out; if the mouse survives, the two drugs are given in sequence Give another 4 mice separately, and the total number of animals is 5; if one animal dies in the l...

Embodiment 3

[0036] In this embodiment, the patch clamp experiment is used to detect the inhibitory effect of the total flavonoids of dragon's blood on the Kv1.3 channel function of mammals. The specific process is as follows:

[0037] Human leukemia lymphocyte line Jurkat T cells and HEK-293T cells (Wuhan University Collection Center) were cultured in RPMI 1640 ( Hyclone) and high-glucose DMEM (Hyclone) medium, at 37°C, 5% CO 2 cultured in an incubator. The eukaryotic expression vector pIRES2-EGFP (provided by the laboratory of Professor Li Wenxin of Wuhan University) encoding voltage-gated potassium channels Kv1.1, Kv1.2 and Kv1.3 was transfected into HEK-293T with lipofectamine 2000 (Invitrogen) In cells, green fluorescent cells were selected for electrophysiological tests after 24 hours. The composition of extracellular fluid (mmol / L) used to record voltage-gated potassium channel current in the experiment is: 140NaCl, 5KCl, 2CaCl 2 ,1MgCl 2 , 10HEPES, 10D-Glucose, the pH value of ...

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Abstract

The invention provides a preparation method of total flavonoids from resina draconis. The method comprises the steps of pulverizing the resina draconis medicine into coarse powder, adding a mixed solvent of ethanol and ethyl acetate, conducting reflux extraction twice at a temperature of 50-69 DEG C, and requiring 15-29 minutes each time. In addition, the invention also provides the application ofthe total flavonoids from the resina draconis in the preparation of drugs for preventing and treating autoimmune diseases, slimming drugs, tumor diseases, acute and chronic respiratory disease drugsand cardiovascular disease drugs. The total flavonoids extracted from the resina draconis has less toxic to animals than the resina draconis, and the total flavonoids from the resina draconis has stronger activity of blocking a Kv1.3 potassium channel than the resina draconis, which indicates that the total flavonoids from the resina draconis achieves the enrichment of active components, eliminates impurities which may cause toxic side effects, improves the safety and enhances the blocking effect on the Kv1.3 potassium channel; in addition, the affinity of total flavonoids from the resina draconis to the Kv1.3 potassium channel is higher than loureirin B, and a synergistic effect of the active components of blocking the Kv1.3 potassium channel in the total flavonoids from the resina draconis is achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method and application of total flavonoids of dragon's blood. Background technique [0002] The normal immune response of the body can effectively resist the intrusion of external pathogenic microorganisms. When the body's immune function is disordered and autoimmune antigens appear, the body's immune system will produce autoantibodies, which can cause a variety of autoimmune diseases by destroying its own organs and tissues. Since the exact pathogenesis of autoimmune diseases has not yet been clarified, there is currently no complete cure in clinical practice. Steroid traditional immunosuppressants are often used, such as cyclophosphamide, dexamethasone, etc. These hormone drugs have significant anti-inflammatory effects and can quickly improve the clinical symptoms of patients with autoimmune diseases, but these drugs cannot eliminate the cause, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61P37/02A61P19/02A61P29/00A61P1/00A61P1/04A61P3/10A61P3/04A61P35/00A61P11/00A61P11/06A61P9/00A61P9/10A61K31/12
Inventor 尹世金冷骏峰王少兵夏家荣石书娟龙思如
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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