Functional mulberry leaf oligosaccharide and preparation method and application thereof

Abstract

The invention discloses functional mulberry leaf oligosaccharide and a preparation method and application thereof. The method comprises the following steps that 1, dried mulberry leaves serve as raw materials, after crushing is conducted, degreasing is conducted by means of petroleum ether, and degreased mulberry leaf powder is obtained; 2, a mulberry leaf polysaccharide extracting solution is obtained by conducting water extraction on the degreased mulberry leaf powder; 3, hydrolase is added in the obtained mulberry leaf polysaccharide extracting solution, and oligosaccharide hydrolysate is obtained through enzymolysis; 4, ethanol is added in the oligosaccharide hydrolysate, unreacted mulberry leaf polysaccharide is subjected to alcohol precipitation, and a mulberry leaf oligosaccharide solution with polysaccharide removed is obtained; 5, saccharomyces cerevisiae is added in the mulberry leaf oligosaccharide solution with the polysaccharide removed, fermentation is conducted to removemonosaccharide, and finally the functional mulberry leaf oligosaccharide is obtained. Accordingly, alcohol precipitation is utilized for removing the polysaccharide, microbial fermentation is utilized for removing the monosaccharide, the mulberry leaf oligosaccharide separation and purification process is simplified, the functional mulberry leaf oligosaccharide has better economical efficiency and environmental friendliness, and the obtained mulberry leaf oligosaccharide has the physiological efficacy of promoting proliferation of intestinal beneficial bacteria and inhibiting growth of harmful bacteria.

Description

technical field [0001] The invention relates to a functional mulberry leaf oligosaccharide and its preparation method and application. Background technique [0002] Functional oligosaccharides are difficult to be digested and absorbed by the gastrointestinal tract, have low sweetness and low calories, and basically do not increase blood sugar and blood lipids. They can promote the proliferation of probiotics, inhibit harmful bacteria, prevent and assist chronic diseases, enhance the body's immune function and promote Various physiological regulation functions such as mineral absorption have attracted much attention and become the focus of research. [0003] As a new functional sugar source, oligosaccharides are widely used in food, health products, beverages, medicines, feed additives and other fields. It has developed into an important biotechnology industry, giving birth to a functional food market of more than 30 billion US dollars and a functional feed market of more th...

AI Technical Summary

Problems solved by technology

However, the current research on polysaccharides has the following problems: 1) due to its large molecular weight and viscosity, resulting in poor solubility and low bioavailability; 2) digestive enzymes in the human body such as α-amylase, glucosidase, maltase, iso Maltase, sucrase, etc. are very specific to glycosidic bonds, and can only hydrolyze glycosidic bonds in the α-1,4 configuration, while mulberry leaf polysaccharides contain a large amount of β-configuration, α-1,2, α- 1,3, α-1,5 glycosidic bonds are difficult to be absorbed and utilized by the human body; 3) Polysaccharides are degraded into functional oligosaccharide fragments in the body to be absorbed and play a role

[0005] At present, in the preparation of oligosaccharides from polysaccharides, traditional chemical methods such as acid hydrolysis and oxidative hydrolysis are still the main method. The disadvantages are high corrosion to equipment, large environmental pollution, and easy destruction of specific functional groups on sugar chains.

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