Haworthia maughanii tissue culture and rapid propagation method based on LED light source
A technology of LED light source and tissue culture rapid propagation, which is applied in the field of plant breeding, can solve the problems of difficult to achieve ideal, deformed tissue culture seedlings, low proliferation rate, etc., and achieve the effect of shortening the proliferation cycle, good growth and large quantity
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Embodiment 1
[0029] The invention provides a method for tissue culture and rapid propagation of Twelve Volumes of Mao Han based on an LED light source. The specific steps of the method are as follows:
[0030] 1. Explant selection
[0031] Select the mature leaves of the plant type plump, strong and free of diseases and insect pests as the explants.
[0032] 2. Primary culture: disinfection and inoculation of explants
[0033] Cut the roots of the Maohan Twelve Juan plant (keep the plant intact) and wash it, wash it with washing powder, rinse it with running water for 1-2 hours, transfer the explant to the sterile operating table, and rinse it twice with sterile water first, Disinfect with 75% alcohol for 20 seconds, rinse with sterile water for 1-2 times, then disinfect with 0.1% mercuric chloride for 6 minutes, and rinse with sterile water for 6-8 times, then peel off the leaves, which should have a base tissue, the leaves were directly connected to the callus induction medium, and ...
Embodiment 2
[0045] The invention provides a method for tissue culture and rapid propagation of Twelve Volumes of Mao Han based on an LED light source. The specific steps of the method are as follows:
[0046] 1. Explant selection
[0047]Select the mature leaves of the plant type plump, strong and free of diseases and insect pests as the explants.
[0048] 2. Primary culture
[0049] Disinfection and inoculation of explants
[0050] Cut the root of the Maohan Twelve Juan plant (keep the plant intact) and wash it, wash it with washing powder, rinse it with running water for 1-2 hours, transfer the explant to the sterile operating table, and rinse it twice with sterile water first, Disinfect with 75% alcohol for 20 seconds, rinse with sterile water for 1-2 times, then disinfect with 0.1% mercuric chloride for 6 minutes, rinse with sterile water for 6-8 times, peel off the leaves, and the leaves should have a base tissue, the leaves were directly connected to the callus induction mediu...
Embodiment 3
[0062] The invention provides a method for tissue culture and rapid propagation of Vientiane Vientiane based on LED light sources. The method adopts the method of selecting mature leaves of Vientiane Vientiane plants with plump plant type, robustness and no pests and diseases as explants, and the explants are sterile After the treatment, the explants were inoculated on the primary culture callus induction medium, and cultured under appropriate temperature, humidity and light intensity conditions to induce callus tissue, and induce differentiation into clustered buds under the irradiation of specific LEDs, etc. The specific steps are:
[0063] 1. Explant selection
[0064] Select the mature leaves of the plant type plump, strong and free of diseases and insect pests as the explants.
[0065] 2. Primary culture
[0066] Disinfection and inoculation of explants
[0067] Cut the root of the Maohan Twelve Juan plant (keep the plant intact) and wash it, wash it with washing po...
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