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Preparation and application of PEG/Dex dual-aqueous-phase based calcium carbonate/calcium alginate composite microcapsules

A technology of calcium alginate and calcium carbonate, which is applied in the direction of microsphere preparation, microcapsule preparation, application, etc., and can solve the problems that restrict the wide application of calcium alginate microcapsules, the batch of recycled use is not high, and the mechanical strength of microcapsules is poor. , to achieve the effect of not easy to swell during storage time, more cycle times, and less equipment requirements

Active Publication Date: 2019-05-03
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

V.Breguet et al introduced a method (XVth International Workshop on Bioencapsulation, Vienna, Au. Sept 6-8, 2007.) utilizing casein and alginate as two aqueous phases to make calcium alginate microcapsules, but it uses The way of co-extrusion, the disadvantages caused by this technology are inevitable
[0005] Calcium alginate microcapsules in the prior art have been widely used in drug delivery and immobilized enzyme carriers due to their excellent biocompatibility. However, due to their own physical and chemical properties, the microcapsules have poor mechanical strength, large pore size, and easy Swelling and other shortcomings lead to easy leakage of the package when it is used as a drug carrier, and the recycling batch is not high when used as an immobilized enzyme carrier. These problems have always restricted the application of calcium alginate microcapsules in pharmaceutical and food industries. widely used

Method used

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  • Preparation and application of PEG/Dex dual-aqueous-phase based calcium carbonate/calcium alginate composite microcapsules
  • Preparation and application of PEG/Dex dual-aqueous-phase based calcium carbonate/calcium alginate composite microcapsules
  • Preparation and application of PEG/Dex dual-aqueous-phase based calcium carbonate/calcium alginate composite microcapsules

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Embodiment 1

[0046] figure 1 It is the mechanism of calcium carbonate / calcium alginate composite microcapsules produced by the interface reaction of two-phase liquid droplets in the present invention, that is, a calcium carbonate / calcium alginate composite microcapsule prepared based on PEG / Dex two-water phase and its preparation method Include the following steps:

[0047] 1. Configure polyethylene glycol / dextran aqueous two-phase

[0048] (1) prepare 16wt% polyethylene glycol solution, molecular weight 8kDa, denoted as A solution;

[0049] (2) prepare 16wt% dextran solution, molecular weight 500kDa, denoted as B solution;

[0050] (3) Take the same volumes of solution A and solution B and mix them well, let stand for 18-24 hours to separate the phases, extract the upper phase and lower phase respectively and transfer them to two beakers for later use, and record them as solution C and solution D respectively.

[0051] 2. Preparation of calcium carbonate / calcium alginate composite micr...

Embodiment 2

[0057] 1. Configure polyethylene glycol / dextran aqueous two-phase

[0058] (1) prepare 16wt% polyethylene glycol solution, molecular weight 8kDa, denoted as A solution;

[0059] (2) prepare 16wt% dextran solution, molecular weight 500kDa, denoted as B solution;

[0060] (3) Take the same volumes of solution A and solution B and mix them well, let stand for 18-24 hours to separate the phases, extract the upper phase and lower phase respectively and transfer them to two beakers for later use, and record them as solution C and solution D respectively.

[0061] 2. Preparation of calcium carbonate / calcium alginate composite microcapsules

[0062] (4) Solution E: Weigh 0.108g of sodium alginate and 0.03g of urease, add them to 10mL of solution C, mix well, and let stand as a receiving solution;

[0063] (5) Solution F: Weigh 0.02g of anhydrous calcium chloride and 0.02g of urea and dissolve them in 2mL of solution D, mix well, and let stand as an injection;

[0064] (6) Use an or...

Embodiment 3

[0068] 1. Configure polyethylene glycol / dextran aqueous two-phase

[0069] (1) prepare 16wt% polyethylene glycol solution, molecular weight 8kDa, denoted as A solution;

[0070] (2) prepare 16wt% dextran solution, molecular weight 500kDa, denoted as B solution;

[0071] (3) Take the same volumes of solution A and solution B and mix them well, let stand for 18-24 hours to separate the phases, extract the upper phase and lower phase respectively and transfer them to two beakers for later use, and record them as solution C and solution D respectively.

[0072] 2. Preparation of calcium carbonate / calcium alginate composite microcapsules

[0073] (4) Solution E: Weigh 0.108g of sodium alginate and 0.03g of urease, add them to 10mL of solution C, mix well, and let stand as a receiving solution;

[0074] (5) Solution F: Weigh 0.06g of anhydrous calcium chloride and 0.02g of urea and dissolve them in 2mL of solution D, mix well, and let stand as an injection;

[0075] (6) Use an ordi...

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Abstract

The invention discloses preparation and application of PEG / Dex dual-aqueous-phase based calcium carbonate / calcium alginate composite microcapsules. The composite microcapsules are produced by taking dual-aqueous-phase (glucan / polyethylene glycol) liquid droplets as a template. The composite microcapsules are good in monodispersity, and the particle size can be accurately regulated and controlled from several microns to several millimeters. The microcapsules are of hollow structures, capsule walls are of double layers, the thickness of the capsule walls is controllable (about 1 / 50 to 1 / 4 the particle size of the microcapsules), inner sides of the capsules are of dense calcium carbonate layers, and outer sides of the capsules are of calcium alginate layers. Compared with pure calcium alginate microcapsules, the microcapsules have good mechanical strength and a dense capsule wall structure, the damage to capsule layers caused by a shearing force is avoided, and the leakage of contents isinhibited. The microcapsules can be used for constructing a low-leakage drug transport system and can also be used for preparing an immobilized enzyme carrier, which is high in number of times of recycle, long in preservation time and difficult in swelling. According to the preparation and the application, the application of calcium alginate microcapsules in the fields of biomedicines, food industry, chemical industry for daily use, enzyme engineering and the like is extended.

Description

technical field [0001] The invention belongs to the technical field of new materials, especially the preparation and application of polymer microcapsule materials. Background technique [0002] Microcapsules, a carrier material with a core-shell structure, are widely used as encapsulation systems for protection of active substances, immobilization of cells, immobilization of enzymes, and controlled release of drugs. Alginate, an anionic polysaccharide extracted from seaweed, is one of the most popular biocompatible materials, given its abundance, non-toxicity, non-immunogenicity, good biocompatibility and water solubility, so , alginate has been widely used to make microcapsules. At present, the methods commonly used to make calcium alginate microcapsules include forward drop infusion method, reverse drop infusion method, microfluidic technology and co-extrusion method. [0003] The positive drip infusion method is to drop the sodium alginate solution into the solution con...

Claims

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Application Information

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IPC IPC(8): B01J13/02A61K9/50A61K47/36A61K47/02A61K47/10A61K8/11A61K8/86A61K8/19A61K8/73A61Q19/00A23L33/16A23P10/30C12N11/10C12N11/14
Inventor 孟涛唐启明陈佳琳钱妍羽许晓蕾李彬宇
Owner SOUTHWEST JIAOTONG UNIV
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