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Preparation methods of mineralized hydrogel and biomimetic mineralized bone repair material

A mineralized water and hydrogel technology, applied in the fields of biomedical materials and biomedical engineering, can solve the problems of cell incompatibility, inability to simulate the physiological mineralization process well, and inability to achieve cell in situ encapsulation, etc.

Active Publication Date: 2019-04-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Horiguchi; Y, Moriwaki; H, Kitago; T, Kajimoto; Y, Iwayama, Formation of apatite-collagen complexes. Journal of biomedical materials research 1996,31(1):43-49); Incompatible with cells, in situ encapsulation of cells cannot be achieved, and the physiological mineralization process of cells in bone tissue cannot be well simulated

Method used

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  • Preparation methods of mineralized hydrogel and biomimetic mineralized bone repair material
  • Preparation methods of mineralized hydrogel and biomimetic mineralized bone repair material
  • Preparation methods of mineralized hydrogel and biomimetic mineralized bone repair material

Examples

Experimental program
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Embodiment 1

[0075] Example 1 Mineralized Collagen Hydrogel

[0076] The steps of preparing the mineralized collagen hydrogel in this embodiment are as follows:

[0077] (A1) Preparation of Methacrylated Collagen (Col-MA)

[0078] The reaction system formed by mixing the PBS dispersion of medical collagen (pH 7.5) with methacrylic anhydride was reacted at 0°C for 4 hours under stirring conditions, and the obtained product was dialyzed in deionized water for 3 days and then vacuum-dried to obtain methacrylic anhydride. Collagen; 100 parts of reaction system, the final concentration of Chinese collagen is 0.1%; the final concentration of methacrylic anhydride is 0.003%.

[0079] (A2) Preparation of methacryloyl alkaline phosphatase (ALP-MA)

[0080] The reaction system formed by mixing the PBS dispersion of alkaline phosphatase (pH 7.5) and methacrylic anhydride was reacted at 0°C under stirring conditions for 24 hours, and the obtained product was dialyzed in deionized water for 3 days an...

Embodiment 2

[0085] Example 2 Mineralized Collagen Hydrogel

[0086] The steps of preparing the mineralized collagen hydrogel in this embodiment are as follows:

[0087] (B1) Preparation of Methacrylated Collagen (Col-MA)

[0088] The reaction system formed by mixing the PBS dispersion of medical collagen (pH 7.5) with methacrylic anhydride was reacted for 2 hours at 4°C under stirring conditions, and the obtained product was dialyzed in deionized water for 5 days and then vacuum-dried to obtain methacrylic anhydride. The final concentration of collagen in 100 parts of the reaction system is 0.1%; the final concentration of methacrylic anhydride is 0.01%.

[0089] (B2) Preparation of methacryloyl alkaline phosphatase (ALP-MA)

[0090] The reaction system formed by mixing the PBS dispersion of alkaline phosphatase (pH 7.5) and methacrylic anhydride was reacted at 4°C under stirring conditions for 4 hours, and the obtained product was dialyzed in deionized water for 4 days and then vacuum-...

Embodiment 3

[0095] Example 3 Mineralized gelatin hydrogel

[0096] The steps of preparing mineralized gelatin hydrogel in this embodiment are as follows:

[0097] (B1) Preparation of Acrylylated Gelatin

[0098] The reaction system formed by mixing the sodium acetate buffer dispersion (pH 8.0) of medical gelatin with acrylic anhydride was reacted for 12 hours at 5°C under stirring conditions, and the obtained product was dialyzed in deionized water for 7 days and then vacuum-dried to obtain acrylylated gelatin ; The final concentration of 100 parts of reaction system Chinese medicine gelatin is 2%; the final concentration of acrylic anhydride is 2%.

[0099] (B2) Preparation of methacryloyl alkaline phosphatase

[0100] The reaction system formed by mixing alkaline phosphatase sodium acetate buffer dispersion (pH 8.0) with methacrylic anhydride was reacted for 2 hours at 5°C under stirring conditions, and the obtained product was dialyzed in deionized water for 5 days and then vacuum-dr...

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Abstract

The invention discloses preparation methods of mineralized hydrogel and a biomimetic mineralized bone repair material. The mineralized hydrogel is prepared by using a photoactivated biomacromoleculesand a photoactivated phosphatase as raw materials; hydrogel is formed by initiating cross-linking by means of light irradiation or initiating polymerization cross-linking by means of a radical initiator; when the hydrogel is put into mineralized liquid, phosphatase can promote the uniform deposition of phosphate in the gel, so that the uniformly mineralized hydrogel is obtained. Phosphoprotein-like molecules are added during the preparation of the hydrogel; the phosphoprotein-like molecules can promote the uniform deposition of phosphate along a molecular network of biomacromolecules, so thatthe high-strength mineralized hydrogel can be obtained. When the phosphoprotein-like molecules and cells are added during the preparation of the hydrogel, the in-situ loading of the cells can be realized, and the enzyme in a process of bone physiological mineralization can be simulated so as to promote a phosphate mineralization process, so that the biomimetic mineralized bone repair material withhigh strength can be obtained, and the personalized customization of the bionic mineralized bone repair material can be realized; therefore, the preparation methods have a broad application prospectin the field of bone tissue engineering.

Description

technical field [0001] The invention belongs to the technical fields of biomedical materials and biomedical engineering, and relates to the preparation of mineralized hydrogel and bionic mineralized bone repair materials. Background technique [0002] Bone tissue is a highly differentiated connective tissue with a micro-to-macro hierarchical structure, in which mineralized collagen fibers are the basic building blocks of natural bone tissue. [0003] Alkaline phosphatase is an important component in the extracellular matrix of bone tissue and participates in the nucleation, growth and structure regulation of apatite mineral phase; alkaline phosphatase participates in matrix mineralization mainly through enzymatic hydrolysis of pyrophosphate in the matrix Phosphate ions, thereby adjusting the balance between inorganic phosphate ions and pyrophosphate in the matrix, promoting the combination of extracellular matrix and calcium ions to produce a mineralized microenvironment, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/22A61L27/20A61L27/12A61L27/38A61L27/50A61L27/52
CPCA61L27/12A61L27/20A61L27/222A61L27/227A61L27/24A61L27/3821A61L27/3834A61L27/50A61L27/52A61L2420/02C08L5/04C08L5/08C08L5/02C08L89/00
Inventor 范红松陈露孙静罗红蓉
Owner SICHUAN UNIV
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