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Development and Application of Nisin Solution Stabilizer

A technology of stabilizer and solution, applied in the field of preparation and application of Nisin solution stabilizer, can solve the problem of high production cost, achieve the effects of good protection, simple configuration and operation, and low production cost

Active Publication Date: 2022-08-05
ZHEJIANG SILVER ELEPHANT BIO ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Production costs of microemulsion preparations, nanoparticle preparations, bioadhesive particles, etc. are relatively high

Method used

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  • Development and Application of Nisin Solution Stabilizer
  • Development and Application of Nisin Solution Stabilizer
  • Development and Application of Nisin Solution Stabilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Accurately weigh 0.5g of pure Nisin (content 98%), add 0.1%, 0.5%, 1%, 2%, 5% of methionine, dissolve in 50mL of 0.02M hydrochloric acid, and respectively take 10mL into a stoppered test tube , and stirred at room temperature for 15 min, and finally adjusted the pH to 3.5 with 200 mM, pH=7.4 phosphate buffer. After stirring evenly, all samples were placed in a 55 °C incubator, and a control experiment was carried out at the same time. Samples were taken on 7 days and 14 days respectively, and the content of Nisin was determined by the biological titer method. The determination method was operated according to the national standard NY / T392-2000. The results are shown in Table 1.

[0025] Table 1 Residual amount of Nisin in concentrated solution under the action of methionine (mg / mL)

[0026]

[0027] It can be seen from the data in Table 1 that methionine has a certain degree of protective effect on the Nisin concentrate. Compared with the reference substance, adding ...

Embodiment 2

[0029] Accurately weigh 0.5g of pure Nisin (content 98%), add 0.1%, 0.5%, 1%, 2%, 5% of chitosan, dissolve in 50mL of 0.02M hydrochloric acid, and respectively take 10mL into a stoppered test tube, And stirred at room temperature for 15min, and finally adjusted the pH to 3.5 with 200mM, pH=7.4 phosphate buffer. After stirring evenly, all samples were placed in a 55°C incubator, and a control experiment was carried out at the same time. Samples were taken on 7 days and 14 days respectively, and the content of Nisin was measured by the biological titer method. The measurement method was operated according to the national standard NY / T392-2000. The results are shown in Table 2.

[0030] Table 2 Residual amount of Nisin in concentrated solution under the action of chitosan (mg / mL)

[0031]

[0032] It can be seen from the data in Table 2 that chitosan has a certain degree of protective effect on the Nisin concentrate. Compared with the reference substance, adding 1% chitosan in...

Embodiment 3

[0034] Accurately weigh 0.5g of normal Nisin product (content 98%), add 0.1%, 0.5%, 1%, 2%, 5% of low-ester pectin respectively, dissolve in 50mL of 0.02M hydrochloric acid, respectively take 10mL to a stoppered test tube and stirred at room temperature for 15 min, adjusted to the same volume by adding solvent to CK, and finally adjusted the pH to 3.5 with 200 mM, pH=7.4 phosphate buffer. After stirring evenly, all samples were placed in a 55 °C incubator, and a control experiment was carried out at the same time. . Samples were taken on 7 days and 14 days respectively, and the content of Nisin was determined by the biological titer method. The determination method was operated according to the national standard NY / T392-2000. The results are shown in Table 3.

[0035] Table 3 Residual amount of Nisin in concentrated solution under the action of low-ester pectin (mg / mL)

[0036]

[0037] It can be seen from the data in Table 3 that low-ester pectin has a certain degree of p...

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Abstract

The application provides a long-acting stabilizer during the production and storage of Nisin solution, which belongs to the technical field of microorganism preservation. Mix 1-10g of nisin (Nisin pure product) with 0.1-2g of methionine, or low-ester pectin, or chitosan, and then use 0.02M hydrochloric acid to make up to 100mL, and then use 200mM phosphate buffer. Adjust the pH to 2.0~4.0; in the production process of Nisin, add 0~10kg of methionine, or low-ester pectin, or chitosan to the 5000L acidification tank and mix, then add hydrochloric acid to adjust the pH to 1.9, 80℃ After 1 h of incubation, the temperature was lowered to 50 °C, and then the ceramic membrane was sterilized by filtration. The stabilizer of the invention is cost-effective, can be used for maintaining the activity and long-term effect of Nisin solutions in the fields of food, cosmetics and the like, and can be used in the production process of Nisin to reduce the damage of Nisin in the process of acidification.

Description

technical field [0001] The invention relates to the technical field of microorganism preservation, in particular to the preparation and application of three Nisin solution stabilizers. Background technique [0002] Nisin (Nisin), also known as nisin or transliterated as nisin, is a polypeptide substance produced by nisin. It consists of 34 amino acid residues and has a molecular weight of about 3400Da. Since nisin can inhibit most Gram-positive bacteria and has a strong inhibitory effect on the spores of Bacillus, it is widely used in the food industry as a food preservative. . [0003] In industrial production, Nisin is often easily degraded or destroyed due to the influence of external conditions during the preparation, storage and release of its preparations, resulting in large economic losses. Therefore, it is very important to improve the stability of the protein. . Nisin is a light brown solid powder that needs to be dissolved in water or liquid when used, and has d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/315C07K1/00
CPCC07K14/315
Inventor 王子琦陈小龙陆跃乐张栋金陈斌李光进洪超群陈艺强
Owner ZHEJIANG SILVER ELEPHANT BIO ENG
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