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Phase change type nano particle and preparation method and application thereof

A nanoparticle, phase-change technology, applied to nanoparticles, phase-change nanoparticles. It can solve the problems of general imaging effect of gold nanorods, limitation of drug delivery routes, and low biocompatibility.

Active Publication Date: 2019-04-16
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The technical solution of this patent has the following problems: (1) the imaging effect of the gold nanorod itself is average, and its tumor treatment can only be realized through the photothermal effect of the gold nanorod; (2) the film-forming material encapsulating the gold nanorod is two Silicon oxide, the material has low biocompatibility and has certain immunogenicity, and it is difficult for the gold nanoparticles formed by encapsulating the above-mentioned film-forming materials to enter the lesion tissue through the vascular endothelial cells, so that the route of administration is restricted. limit

Method used

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  • Phase change type nano particle and preparation method and application thereof
  • Phase change type nano particle and preparation method and application thereof
  • Phase change type nano particle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Preparation of phase-change nanoparticles

[0048] (1) Main reagents

[0049] Gold nanorods: Hong Kong Nanoseedz Company;

[0050] Dipalmitoylphosphatidylcholine (1,2-dihexadecanoyl-rac-glycero-3-phosphocholine, DPPC), distearoylphosphatidylglycerol (1,2-distearoyl-sn-glycero-3-phospho-(1- rac-glycerol), DSPG), distearoylphosphatidylethanolamine-polyethylene glycol-active ester (1,2-distearoyl-sn-glycero-3-phosphoethanolamineN-[methoxy(polyethylene glycol)]-2000-NHS, DSPE-PEG2000-NHS): American Avanti Company;

[0051] Cholesterol, liquid fluorocarbon (PFH), dichloromethane, isopropanol; 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinyl Amine (NHS), MES buffer, PEG, KI, L-Cysteine: Sigma, USA;

[0052] Monoclonal antibody against melanoma-associated antigen (MAGE-1-Antibody): China Bioye;

[0053] Mouse melanoma cell line B16 was obtained from Wuhan Punuosai.

[0054] (2) Main equipment

[0055] Malvern particle si...

Embodiment 2

[0074] Example 2: Preparation of phase-change nanoparticles without surface modification of gold nanorods

[0075] The experimental reagents, equipment and methods of this example are basically the same as in Example 1, except that no cysteine ​​and potassium iodide are used to modify the gold nanorods before preparing the phase-change nanoparticles. The encapsulation efficiency of the nanoparticles prepared this time was (65.23±5.79)%. The in vitro photoacoustic imaging results were as follows: Figure 9 (nanoparticle concentration is 5mg / ml), Figure 10 (nanoparticle concentration is 20mg / ml). The encapsulation efficiency of the nanoparticles prepared in this example is lower than that in Example 1, and the photoacoustic imaging signal intensity of the nanoparticles with the same concentration in this example is lower than that in Example 1.

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Abstract

The invention belongs to the technical field of medicines and relates to a nano particle, in particular to a phase change type nano particle. The phase change type nanoparticle is formed by liposome-encapsulated gold nanorods and liquid fluorocarbon, wherein monoclonal antibodies of melanoma-associated antigens are attached to the surfaces of liposomes. The phase change type nanoparticle has phototransformation properties, thereby having superior development and tumor treatment effects; drug encapsulation of the phase change type nano particle is achieved by using a liposome material, so thatthe immunity of the nanoparticle is reduced, and he biocompatibility of the nanoparticle is improved. The nanoparticle prepared by the technical scheme can be applied to contrast agents and therapeutic drugs of melanoma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and relates to a nano particle, in particular to a phase change type nano particle. Background technique [0002] Photoacoustic imaging is a non-invasive medical imaging method. When pulsed laser light is irradiated into biological tissue, the light-absorbing domain of the tissue will generate ultrasonic signals. The application of targeted nanoscale biomaterials to the field of photoacoustic imaging can not only enhance the detection signal and improve detection efficiency, but also realize disease diagnosis and precise treatment at the molecular level. Gold nanorods are a new type of multifunctional nanomaterials. Nanorods have continuously adjustable resonance wavelengths, strong optical extinction characteristics, good photothermal conversion efficiency, and good stability. The excellent properties of gold nanorods can be developed Phase-change nanoparticles are widely used in photoacousti...

Claims

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Application Information

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IPC IPC(8): A61K49/22A61K41/00A61K9/51A61K47/24A61K47/20A61K47/02A61K47/68A61K47/69A61P35/00
CPCA61K41/0052A61K47/68A61K47/6929A61K49/223A61K49/225A61P35/00A61K9/5115A61K9/5123A61K9/5146
Inventor 李雪霖张兴平王志刚冉海涛李攀
Owner CHONGQING MEDICAL UNIVERSITY
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