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Methanomassiliicoccus sp. and application thereof

A methane bacteria, methane technology, applied in the field of methane bacteria, can solve the problems of meat and milk quality and human health hazards, and achieve the effect of reducing toxicity

Active Publication Date: 2019-04-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, choline supplementation will increase the content of trimethylamine oxide in blood, urine, skeletal muscle and milk, causing harm to the quality of meat and milk and human health

Method used

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  • Methanomassiliicoccus sp. and application thereof
  • Methanomassiliicoccus sp. and application thereof
  • Methanomassiliicoccus sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Screening strain LGM-RCC1

[0019] 1. Materials and methods

[0020] 1.1 Inoculum

[0021] Goat fresh rumen juice.

[0022] 1.2 Medium composition and its preparation

[0023] 1.2.1 Medium composition

[0024] The composition of the methanogen BRN medium is shown in Table 1. The experiments of the present invention are all carried out with BRN medium.

[0025] Table 1 Methanogen BRN medium composition (1L formula)

[0026]

[0027] Note: The above solution solvents are all water.

[0028] 1.2.2 Cell-free rumen juice

[0029] The fresh goat rumen fluid passes through the rumen fistula, is collected by a negative pressure device, and then filtered through two layers of gauze. The liquid is centrifuged at 12000 rpm at 4°C for 20 minutes, and the supernatant is taken and stored at -20°C for later use.

[0030] 1.2.3 Volatile fatty acid solution

[0031] Accurately measure acetic acid, 6.85mL; propionic acid, 3.0mL; butyric acid, 1.84mL; 2-methylbutyric acid, 0.55mL; isobutyric ac...

Embodiment 2

[0044] Example 2 LGM-RCC1 uses different substrates to produce methane

[0045] The composition and preparation method of BRN medium are the same as in Example 1, except that the substrates of the fermentation medium are methanol (MeOH, 0.06mol / L), monomethylamine (MMA, 0.06mol / L), and dimethylamine. (DMA, 0.03mol / L), trimethylamine (TMA, 0.02mol / L) or methyl 3-methylthiopropionate (M 3 PT, 0.06mol / L). Strain LGM-RCC1 inoculum preparation medium adopts BRN medium, the substrate is a mixture of five methyl compounds (each substrate is 0.01 mol / L), cultured at 39°C for 20 days and inoculated. After that, the strains were connected to the fermentation tube of 10mL BRN medium preheated to 39℃ and containing penicillin, vitamin solution and substrate solution. The fermentation tube was then fed with 100Kpa hydrogen gas, cultivated at 39℃ and measured for methane Yield, see results figure 1 .

[0046] Such as figure 1 As shown, LGM-RCC1 can use hydrogen to reduce methanol, monomethylam...

Embodiment 3

[0047] Example 3 Effect of pH on LGM-RCC1 Methane Production

[0048] The composition and preparation method of the BRN medium, the preparation method of the inoculum (medium pH 6.9-7.0), the fermentation is the same as in Example 2, except that the substrate is only trimethylamine, and the concentration is 0.02mol / L. The pH of the culture medium in the fermentation stage is set to 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, and the substrate is trimethylamine. The strain is preheated to 39°C, and contains penicillin and vitamin solution. , Trimethylamine (0.02mol / L) solution in the fermentation tube of 10mL BRN medium, the fermentation tube was then fed with 100Kpa hydrogen, cultivated at 39℃ and measured the methane production, the results are shown figure 2 , LGM-RCC1 can grow between pH 6.5-8.0.

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Abstract

The invention discloses methanomassiliicoccus sp. The classification name of the methanomassiliicoccus sp. is methanomassiliicoccus sp. (methanomassiliicoccus sp.), the bacterial strain number is LGM-RCC1, the methanomassiliicoccus sp. is preserved in the China General Microbiological Culture Collection Center, the preservation time is at 24 October, 2018, and the preservation number is CGMCC No.16819. The invention also discloses an application of the methanomassiliicoccus sp. in fermentation production of methane, and an application of the methanomassiliicoccus sp. to preparation of a probiotic agent or a feed additive. The LGM-RCC1 can be used for generating the methane through reducing methanol, monomethylamine, dimethylamine, trimethylamine and methyl 3-methylthiopropionate through hydrogen, and the LGM-RCC1 can be used as an additive for increasing the yield of the methane in a methane-generating pit; and the LGM-RCC1 can utilize the trimethylamine in digestive tracts, toxicity of the LGM-RCC1 to human bodies or animals can be reduced, and the LGM-RCC1 is a potential probiotic for treating diseases and preventing cardiovascular diseases caused by trimethyl amine oxide.

Description

Technical field [0001] The invention relates to a methane bacterium, in particular to a methane bacterium and its application. Background technique [0002] Food is rich in choline, carnitine, betaine and lecithin. These substances are rapidly degraded by microorganisms in the digestive tract of animals or humans to produce trimethylamine, which reaches the liver through blood circulation. Normal humans and animals convert trimethylamine into trimethylamine oxide in the liver, but recent studies have found that trimethylamine oxide can increase the risk of cardiovascular disease. People who can't metabolize trimethylamine will suffer from trimethylamineuria, which emits a fishy smell. There is no specific treatment. Choline is an important feed additive, usually used in the feed of pigs, dairy cows, sheep and other animals to improve animal production performance, increase animal feed energy utilization, reduce the risk of fatty liver disease, and relieve heat stress Excitement...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P5/02A61K35/741A61P9/00A23K10/18C12R1/01
CPCA61K2035/115A23K10/18A61P9/00C12P5/023C12N1/205C12R2001/01Y02E50/30
Inventor 金巍成艳芬朱伟云
Owner NANJING AGRICULTURAL UNIVERSITY
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