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High sensitivity detection method for silk cultural relic

A high-sensitivity, detection-method technology, applied in measuring devices, instruments, and material analysis through electromagnetic means, can solve problems such as the difficulty of detecting cultural relics with extremely low silk fibroin content, and achieve high binding rate and utilization efficiency High, large specific surface area effect

Active Publication Date: 2019-04-02
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current advanced technology for protein detection is based on antibody-antigen Western Blotting or ELISA, but these methods are still difficult to detect some artifacts with extremely low silk fibroin content

Method used

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  • High sensitivity detection method for silk cultural relic
  • High sensitivity detection method for silk cultural relic

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A) Take 5g silkworm cocoons, cut them open with scissors, take out the silkworm chrysalis inside, and remove the innermost chrysalis lining. The obtained cocoon shells were weighed and added with a mass fraction of 0.4% Na 2 CO 3 For the degumming solution, stir for 0.5h at a bath ratio of 1:45 and a temperature of 98°C, and repeat this operation twice to fully remove sericin. The insoluble silk fibroin was washed five times with deionized water, and placed in an oven at 55 °C to dry overnight, and reserved for future use.

[0041] B) Add the dried silk fibroin to the protein extract solution (the molar ratio of calcium chloride: water: ethanol is 1:8:2), stir and dissolve for 1.5 h at a bath ratio of 1:45 and a temperature of 98 °C . After cooling and filtering the extract containing silk fibroin at room temperature, dialyze in deionized water with a cellulose dialysis bag with a molecular weight cut-off of 10,000 to remove calcium ions and chloride ions, and change...

Embodiment 2

[0055] A) Take 5g silkworm cocoons, cut them open with scissors, take out the silkworm chrysalis inside, and remove the innermost chrysalis lining. Weigh the obtained cocoon shells and add 0.5% Na by mass fraction 2 CO 3 For the degumming solution, stir for 0.5h at a bath ratio of 1:50 and a temperature of 99°C, and repeat this operation twice to fully remove sericin. The insoluble silk fibroin was washed five times with deionized water, and placed in an oven at 60 °C to dry overnight, and reserved for future use.

[0056] B) Add the dried silk fibroin to the protein extract (the molar ratio of calcium chloride: water: ethanol is 1:8:2), stir and dissolve for 2 h at a bath ratio of 1:50 and a temperature of 99 °C . After cooling and filtering the extract containing silk fibroin at room temperature, dialyze in deionized water with a cellulose dialysis bag with a molecular weight cut-off of 10,000 to remove calcium ions and chloride ions, and change the water every 4 h, 72 h ...

Embodiment 3

[0068] A) Take 5g silkworm cocoons, cut them open with scissors, take out the silkworm chrysalis inside, and remove the innermost chrysalis lining. Weigh the cocoon shells obtained and add 0.6% Na by mass fraction 2 CO 3 The degumming solution was stirred for 0.5h at a bath ratio of 1:55 and a temperature of 100°C, and this operation was repeated twice to fully remove sericin. The insoluble silk fibroin was washed five times with deionized water, and placed in an oven at 55-65°C to dry overnight, and reserved for future use.

[0069] B) Add the dried silk fibroin to the protein extract (the molar ratio of calcium chloride: water: ethanol is 1:8:2), stir and dissolve for 2.5 h at a bath ratio of 1:55 and a temperature of 100 °C . After cooling and filtering the extract containing silk fibroin at room temperature, dialyze in deionized water with a cellulose dialysis bag with a molecular weight cut-off of 10,000 to remove calcium ions and chloride ions, and change the water ev...

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Abstract

The invention relates to the field of cultural relic detection, and discloses a high sensitivity detection method for a silk cultural relic. The method comprises the following steps of: preparing puresilk fibroin powder, and simultaneously extracting the silk fibroin in a cultural sample; sequentially modifying gold nanoparticles, hollow polyacrylic acid-dopamine complex, polymethyl methacrylate-maleic anhydride / 1-octadecene alternating copolymer spherical nanobead, mouse anti-silk fibroin monoclonal antibody on the surface of cleaned glassy carbon electrode by a layer-by-layer self-assemblymethod under the action of NHS / EDC solution; and detecting the silk fibroin solution and the cultural sample solution by using the modified electrode. The high sensitivity detection method for the silk cultural relic has high sensitivity, low detection limit and good reproducibility, and can detect the silk cultural relics with high sensitivity.

Description

technical field [0001] The invention relates to the field of cultural relics detection, in particular to a highly sensitive detection method for silk cultural relics. Background technique [0002] Silk is a natural polymer protein fiber, the origin of which has been shrouded in fog. The conventional detection methods of silk mainly include Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction, etc. However, silk will be affected by many factors if it is kept in the environment of tombs or ruins for a long time, resulting in problems such as protein degradation and macromolecular chain breaks. , it is difficult to detect the existence of silk fibroin by some traditional methods. Therefore, how to use advanced methods of natural science to establish a silk fabric micro-mark detection technology system, and extract ancient silk information from imprints, residues, and soil is very important for the study of the origin of silk. [0003] The current a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3277G01N27/3278
Inventor 李津王秉欧阳毅刘林帅胡智文
Owner ZHEJIANG SCI-TECH UNIV
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