A kind of process method of extracting and purifying stevioside from stevia

Abstract

The invention relates to a process for extracting and purifying steviol glycosides from stevia rebaudiana. Soak the dried stevia rebaudiana leaves in ethanol, press filter, collect the filter residue, and recover ethanol from the filtrate; atomize the stevia leaf slurry through a high-pressure sprayer, use ethanol and / or water as a solvent, extract the target steviol glycoside component, and filter Stevia leaf alcohol water extract is obtained; the obtained stevia leaf alcohol water extract is passed through a ceramic ultrafiltration membrane, and the permeate is collected; the permeate is passed through a ceramic nanofiltration membrane to obtain an extract concentrate; the concentrate is subjected to reverse phase packing chromatography Column purification to obtain raw stevioside sugar; or, the raw sugar is further purified to obtain raw stevioside sugar; purified to obtain raw stevioside sugar and decolorized by gel chromatography to obtain RA raw sugar, RA raw sugar is concentrated and then recrystallized to obtain high-purity RA with a purity of more than 97%. The invention has simple process, controllable cost, strong operability, high product purity and yield, and the final total yield of steviol glycoside is over 95%, which has obvious advantages over the existing traditional process.

Description

technical field [0001] The present invention is designed to relate to a method for stevioside, in particular to a process method for extracting and purifying stevioside from stevia. Background technique [0002] Stevia is a mixture of diterpene glycosides containing 8 components extracted from the leaves of Stevia rebaudiana. The leaves of Stevia rebaudiana contain 8% to 16% of steviol glycosides. Terpene glycosides, whose sweetness is 200 to 300 times that of sucrose, and the calories are only 1 / 300 of that of sucrose, are gradually favored by people due to their high sweetness, low calorie, safety, non-toxicity, and pure nature. Used in food, beverage, medicine, daily chemical industry and other industries. Among the 9 known glycosides of stevioside, the content, taste and sweetness of various components are different, among which stevioside (stevioside), rebaudioside A (RA), rebaudioside C (RC) The content is relatively high, accounting for more than 90%. RA has the hi...

AI Technical Summary

Problems solved by technology

Among them, the high cost of compound enzyme and supercritical extraction is not conducive to industrial production

[0006] It can be seen that the existing water extraction process of stevioside has many impurities in the extract, and it is difficult to separate and purify; the extraction time is long, the water consumption and the amount of organic wastewater produced are large

The traditional extraction of steviol glycosides needs to add a certain amount of expensive preservative TG, which makes separation difficult and increases the cost

The extraction liquid flocculation and impurity removal process adopts chemical flocculation and needs to add flocculants, resulting in a large amount of non-ferrous metal ions in the flocculation liquid, which need to be removed by ion exchange resin, which makes the whole production process cumbersome, long production cycle and high production cost

The current mainstream process of resin adsorption and separation of stevia has low utilization of resin, large consumption of resin, solvent and water, difficult treatment of acid-base wastewater, and low product yield, which has become a pain point in the industry.

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