Method for detecting indoleacetic acid and salicylic acid and electrochemical sensor used in method

Abstract

The invention discloses a method for detecting indoleacetic acid and salicylic acid and an electrochemical sensor used in the method. The electrochemical sensor comprises a three-electrode system anda detection pool, wherein the three-electrode system comprises a working electrode, an Ag / AgCl reference electrode and a platinum wire counter electrode; the working electrode is a modified glassy carbon electrode; and the detection pool is internally filled with a detection base solution of a buffer solution. Electrochemical detection is carried out on IAA (Indole-3-Acetic Acid) and SA (SalicylicAcid) by utilizing a graphene hydrogel modified electrode; the problems that a general chromatographic method and an immunological detection method are complicated to operate, expensive in cost, highin sample size, high in time consumption, and the like, are solved; the real-time detection on the IAA and the SA is realized; the electrochemical sensor has the advantages of being convenient and quick, being high in detection sensitivity, having the detection limit of the IAA being as low as 0.677 mug / ml and the detection limit of the SA being as low as 0.569 mug / ml, and being low in needed sample amount, extremely low in detection cost, little in interference, and the like, and can be used for realizing the fast, simple, convenient and accurate quantitative detection on the IAA and the SA.

Description

technical field [0001] The invention belongs to the technical field of food detection and electrochemical sensors, and in particular relates to a method for detecting indole acetic acid and salicylic acid and an electrochemical sensor used therefor. Background technique [0002] Plant-produced or synthetic plant growth regulator indole-3-acetic acid (IAA) is a small organic molecule that alters plant growth and development. IAA stimulates the growth process, similar to many plant growth regulators such as abscisic acid (ABA), Jasmonic acid (JA) has been widely used in many countries such as Australia, Japan, China, and India, which has drawn more attention to the toxicity and residue issues of its edible plants. Legislative bodies have pointed out maximum residue limits for the protection of health, and reducing the concentration of plant genetic resources residues in commodities as much as possible is one of the most important quality standards in market monitoring and inte...

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Problems solved by technology

Electrochemical sensors currently used to detect IAA and SA are multi-modified electrodes, such as Suriyanarayanan S, Mandal S, Ramanujam K, et al. Electrochemically synthesized molecularly imprinted polythiophene nanostructures as recognition elements for an aspirin-chemosensor[J].Sensors&Actuators B Chemical, 2017, 253, using a sacrificial alumina template on a gold-coated quartz resonator to incorporate a molecularly imprinted polymer (MIP) composed of functional monomers 3-thiophene boronic acid (3-TBA) and 3-thiophene acetic acid (3-TAA) as an electrode, Good results have been achieved in the detection of SA, using methods that can specifically recognize imprinted molecules or molecules with similar structures, these methods based on molecular imprinting require long-term pretreatment of the electrode, and the function There are too few types of monomers to meet the recognition requirements of some molecules; such as Feng Z L, Yao Y Y, Xu JK, et al. One-step co-electrodeposition of graphene oxide doped poly(hydroxymethylated-3,4-ethylenedioxythiophene) film and its electrochemicalstudies of indole-3-acetic acid[J].Chinese Chemical Letters,2014, 25(4):511-516, using one-step co-electrodeposited graphene oxide doped with hydroxymethylation-3,4-ethylenedi Oxythiophene film as an electrode has achieved good results in the detection of IAA, but the selected modified electrode materials are expensive and costly

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