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Electrochemical detection chip and electrochemical sensor, as well as preparation methods and application thereof

A detection chip and electrochemical technology, applied in the field of blood coagulation detection, can solve the problems of difficult transmission of electrons and low electrical signal strength, and achieve the effects of short detection time, high detection sensitivity and simple operation.

Pending Publication Date: 2019-03-22
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects of difficult electron transmission and low electrical signal intensity in the prior art based on electrochemical detection of prothrombin time.

Method used

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  • Electrochemical detection chip and electrochemical sensor, as well as preparation methods and application thereof
  • Electrochemical detection chip and electrochemical sensor, as well as preparation methods and application thereof
  • Electrochemical detection chip and electrochemical sensor, as well as preparation methods and application thereof

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Experimental program
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Embodiment 1

[0066] This embodiment provides an electrochemical detection chip, comprising a base layer 3 and an electrode layer 2 formed on the base layer 3, such as figure 1 As shown, the electrode layer 2 is a working electrode 21 , a counter electrode 22 and a reference electrode 23 . Wherein, the working electrode 21 includes a conductive layer, a nanomaterial layer and a blood coagulation reaction layer which are stacked in sequence. The coagulation reaction layer generates electrical signals for detecting prothrombin time, and the nanomaterial layer transmits and amplifies the electrical signals. The electrochemical detection chip is used to detect prothrombin time. Blood lacking platelets and calcium ions contacts the coagulation reaction layer to convert prothrombin into thrombin, triggering the coagulation process of prothrombin time detection. At the same time, the coagulation reaction layer generates an electrical signal for detecting prothrombin time, and the electrical signa...

Embodiment 2

[0073] This embodiment also provides an electrochemical sensor, such as image 3 As shown, the electrochemical sensor includes the electrochemical detection chip provided in the above-mentioned embodiment 1, and the encapsulation layer 1 disposed on the side of the electrode layer 2 away from the base layer 3 . The material of encapsulation layer 1 is polymethyl methacrylate (PMMA), poly terephthalate (PET), polyetheretherketone (PEEK), polytetrafluoroethylene (PTFE), polyvinyl chloride (PVC), poly Propylene (PP) polymer and the like make the encapsulation layer 1 have stability, oxidation resistance, corrosion resistance, biocompatibility, and certain hydrophilicity. The encapsulation layer 1 is covered on the electrode layer 2 by means of epoxy AB glue, single-sided and double-sided adhesive tape, plasma, ultraviolet light, and thermocompression bonding, so that the encapsulation layer 1 and the electrode layer 2 are tightly bonded. , to reduce the impact of the external en...

Embodiment 3

[0077] This embodiment provides an electrochemical detection chip, the structure of its electrode layer 2 is as follows: Figure 6 As shown, it includes a conductive track 24 arranged on the base layer 3. The conductive track 24 is specifically formed by a conductive silver layer. 3 in the lengthwise direction, finally forming the terminal end of the conductive track 24 on the base layer 3 . The terminal end of the conductive track 24 is connected to the conductive layer of the working electrode 21 and the counter electrode 22. The conductive layer of the working electrode 21 is in the shape of a disc, and the conductive layer of the working electrode 21 is a conductive carbon ink electrode. The counter electrode 22 is in an annular structure spaced apart from the working electrode 21 and surrounding the working electrode 21 , and the counter electrode 22 is a conductive carbon ink electrode. A layer of AgCl is covered on the end portion of the conductive track 24 not connect...

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Abstract

The invention discloses an electrochemical detection chip. The electrochemical detection chip comprises an electrode layer, wherein a working electrode of the electrode layer comprises a conducting layer, a nanomaterial layer and a blood clotting reaction layer which are sequentially laminated, wherein the blood clotting reaction layer is used for producing an electrical signal for detecting prothrombin time; the nanomaterial layer is used for transferring and amplifying the electrical signal. By using high conductivity, large specific area, good biocompatibility and the like of the nanomaterial layer, amplification and enhancement of the electrical signal in the detection process of the prothrombin time are realized so as to improve the sensitivity of chip detection and shorten the detection time. The invention discloses an electrochemical sensor. The electrochemical sensor comprises the electrochemical detection chip. The invention discloses a preparation method of the electrochemical sensor. The preparation method is suitable for preparing the electrochemical sensor with high sensitivity and accurate detection results.

Description

technical field [0001] The invention relates to the technical field of coagulation detection, in particular to an electrochemical detection chip, an electrochemical sensor, a method for modifying a working electrode of the electrochemical detection chip, and a preparation method for the electrochemical sensor. Background technique [0002] Whether the blood coagulation function is normal or not is related to the physiological health of the human body, and the evaluation experiment of blood coagulation function is of great significance to the diagnosis, treatment and prognosis of various diseases. Coagulation time is an important index to measure the coagulation state. Among them, prothrombin time (PT, Prothrombin Time) is a routine index for exogenous coagulation function testing in clinical practice. It is an important indicator for the screening of coagulation diseases, and it is also a preferred indicator for clinical diagnosis and observation of thrombosis and monitoring...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/308G01N27/3271
Inventor 周连群郭振李超张威李传宇姚佳张芷齐李金泽唐玉国
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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