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Composite cellulase system and application of composite cellulase system to producing starch fuel ethanol

A technology of compound cellulase and compound cellulose, which is applied in the direction of biofuels, enzymes, enzymes, etc., can solve the problems of ineffective use of corn cellulose, insufficient degradation of corn starch, and the impact of yeast growth and fermentation. Significant economic benefits, increased speed, total sugar and viscosity reduction effects

Active Publication Date: 2019-03-19
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production process of corn fuel ethanol, corn cellulose has not been effectively utilized, and corn starch has not been fully degraded. In the later stage, problems such as high viscosity of sugar solution and high solid content appear, so the growth and fermentation of yeast are seriously affected.

Method used

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  • Composite cellulase system and application of composite cellulase system to producing starch fuel ethanol
  • Composite cellulase system and application of composite cellulase system to producing starch fuel ethanol
  • Composite cellulase system and application of composite cellulase system to producing starch fuel ethanol

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Effect test

Embodiment 1

[0042] The present invention provides a composite cellulase system prepared by the following method, the method comprising the following steps:

[0043] The Aspergillus niger transformed with the ferulic acid esterase gene derived from Penicillium juniperformis and the dehydrogenase gene derived from Penicillium juniperformis was inoculated with 3% of the inoculation amount in the fermentation medium II to obtain the fermentation broth I. The fermentation broth I is an Aspergillus niger transformant enzyme system containing ferulic acid esterase derived from Penicillium juniperformis and dehydrogenase derived from Penicillium juniperformis, and β-glucosidase derived from Aspergillus niger;

[0044] Inoculating Trichoderma reesei with 3% of the inoculation amount in fermentation medium I to obtain fermentation culture broth II, which contains cellulase derived from Trichoderma reesei; and

[0045] The fermentation broth I and the fermentation broth II are compounded (fully mixed) in a...

Embodiment 2

[0052] The present invention provides a composite cellulase system prepared by the following method, the method comprising the following steps:

[0053] The Aspergillus niger transformed with the ferulic acid esterase gene derived from Penicillium juniperformis and the dehydrogenase gene derived from Penicillium juniperformis was inoculated into fermentation medium II at an inoculum amount of 4% to obtain fermentation broth I. The fermentation broth I is an Aspergillus niger transformant enzyme system containing ferulic acid esterase derived from Penicillium junipera and dehydrogenase derived from Penicillium junipera, and β-glucosidase derived from Aspergillus niger;

[0054] Inoculating Trichoderma reesei in a fermentation medium I at an inoculum amount of 4% to obtain fermentation culture broth II, which contains cellulase derived from Trichoderma reesei; and

[0055] The fermentation broth I and the fermentation broth II are compounded (fully mixed) in a volume ratio of 1:8.5 to ...

Embodiment 3

[0062] The present invention provides a composite cellulase system prepared by the following method, the method comprising the following steps:

[0063] The Aspergillus niger transformed with the ferulic acid esterase gene derived from Penicillium juniperformis and the dehydrogenase gene derived from Penicillium juniperformis was inoculated into fermentation medium II at an inoculum amount of 5% to obtain fermentation broth I. The fermentation broth I is an Aspergillus niger transformant enzyme system containing ferulic acid esterase derived from Penicillium junipera and dehydrogenase derived from Penicillium junipera, and β-glucosidase derived from Aspergillus niger;

[0064] Inoculating Trichoderma reesei at 5% of the inoculum amount to fermentation culture medium I to obtain fermentation culture broth II, said fermentation broth II containing cellulase derived from Trichoderma reesei; and

[0065] The fermentation broth I and the fermentation broth II are compounded (fully mixed) ...

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Abstract

The invention discloses a composite cellulase system and application thereof. A method for preparing the composite cellulase system includes steps of carrying out fermentation culture on aspergillus niger with transformed feruloyl esterase genes from juniper-shaped penicillium and transformed dehydrogenase genes from to juniper-shaped penicillium obtain fermentation culture solution I; carrying out fermentation culture on trichoderma reesei to obtain fermentation culture solution II; compounding the fermentation culture solution I and the fermentation culture solution II according to a certainvolume ratio to obtain the composite cellulase system. The composite cellulase system and the application have the advantages that trichoderma reesei enzyme systems can be effectively improved by thecomposite cellulase system, and the crystalline cellulose degradation speeds of the trichoderma reesei enzyme systems further can be effectively increased; composite cellulase can be added in procedures for producing starch fuel ethanol by means of yeast synchronous saccharification and fermentation, accordingly, the viscosity of corn mash can be reduced, residual celluloses and starch further can be hydrolyzed, corn can be efficiently transformed into the fuel ethanol, and obvious economic benefits can be brought to the ethanol fermentation industry.

Description

Technical field [0001] The invention relates to the technical field of ethanol fermentation production, in particular to a composite cellulase system and its application in the production of starch fuel ethanol. Background technique [0002] Ethanol is an important bulk chemical product with a wide range of uses. It is often used as a fuel, solvent, and disinfectant, as well as other compounds. Ethanol is currently the world's largest fermentation product. my country's emerging industry development plan points out that by 2020, the annual utilization of bio-liquid fuels will reach 12 million tons. Although the production process of starch ethanol fermentation is relatively mature, there are still problems in its production process. In the corn fuel ethanol production process, corn cellulose is not effectively used, corn starch is not fully degraded, and problems such as high sugar liquid viscosity and many solids occur in the later stage, so the growth and fermentation of yeast ...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12N9/42
CPCC12N9/2437C12P7/10Y02E50/10
Inventor 高乐张东远陈树林
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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