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Application of a Pediococcus pentosaceae surface protein in inhibiting food-borne pathogens

A food-borne pathogen, Pediococcus pentosaceae technology, applied in the fields of application, peptide/protein components, antibacterial drugs, etc., can solve the problems of few research reports on the surface protein of lactococcus, and achieve excellent antibacterial efficacy and low investment , enhance the inhibitory effect

Active Publication Date: 2022-03-11
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the structure and function of the surface proteins of lactic acid bacteria mainly focuses on Lactobacillus, including the mucus-binding protein (Mub) of Lactobacillus reuteri, the collagen-binding surface protein (CbsA) and S-layer protein (Slp B) of Lactobacillus crispatus. ), the cell wall anchor protein (CwaA) of Lactobacillus plantarum NL42, etc., and there are relatively few reports on the surface protein of Lactococcus

Method used

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  • Application of a Pediococcus pentosaceae surface protein in inhibiting food-borne pathogens
  • Application of a Pediococcus pentosaceae surface protein in inhibiting food-borne pathogens
  • Application of a Pediococcus pentosaceae surface protein in inhibiting food-borne pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the growth situation and the acid production situation of Pediococcus pentosaceae

[0036] Inoculate -70°C glycerol stock solution in MRS medium with 2% inoculum size, incubate at 37°C for 24 hours, measure the OD every 2 hours 600nm Value and pH value and draw the growth and acid production capacity curve.

[0037] The result is as figure 1 As shown, with the increase of culture time, the growth rate of Pediococcus pentosacea gradually increased and began to enter the logarithmic growth phase at about 4 hours, and reached a stable period after 12 hours of culture, and the growth slowed down. However, in the adaptation period and logarithmic period of Pediococcus pentosaceae, the pH value of the medium has been decreasing, from 5.64 to 4.14, and tended to be stable at 12h. It still maintained stable growth under acidic conditions, indicating that Pediococcus pentosaceae has strong acid adaptability and can survive under low pH conditions.

Embodiment 2

[0038] Example 2: Extraction and analysis of surface protein of P. pentosaceus F28-8

[0039] (1) Extraction method of surface protein:

[0040]The strain was inoculated with MRS medium, cultured at 37°C for 18 hours, collected by centrifugation at 8000 rpm, washed twice with PBS and centrifuged. The bacteria in the control group were resuspended in PBS, and the bacteria in the treatment group were added with 5mol / L LiCl solution (10mL per gram of bacteria) at 37°C for 0.5h, centrifuged (8 000r / min, 15min), the supernatant was discarded, and 4mol / L L guanidine hydrochloride solution (5 mL per gram of bacteria) was resuspended and mixed evenly, treated in a constant temperature incubator at 37 °C for 1 h, centrifuged (8 000 r / min, 15 min), and the supernatant was collected. Transfer the supernatant into a dialysis bag with a molecular cut-off of 14kDa, dialyze in deionized water at 4°C for 48h, and centrifuge (12 000r / min, 15min) to take the supernatant, which is the surface p...

Embodiment 3

[0047] Embodiment 3: Antibacterial application of the surface protein of Pediococcus pentosaceus P.pentosaceus F28-8

[0048] Get the activated Staphylococcus aureus (or Salmonella) bacterial suspension and inoculate it into the LB liquid medium, the inoculum size is 2% (v / v), and the experimental group adds Pediococcus pentosaceae surface protein simultaneously (final concentration is 10 μ g / v). mL), while the control group was only inoculated with Staphylococcus aureus (or Salmonella), cultured on a shaker at 37°C for 10 hours, and measured OD every 2 hours 600nm value. Draw a growth curve graph. At the same incubation time, the absorbance value of the experimental group was recorded as A T , the absorbance value of the control group is recorded as A C , calculate the growth inhibition rate of Staphylococcus aureus (or Salmonella) according to the formula.

[0049]

[0050] Statistical analysis of data: SPSS 13.0 software was used for statistical analysis of data, and...

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Abstract

The invention discloses an application of a surface protein of Pediococcus pentosaceae in inhibiting food-borne pathogenic bacteria. The surface protein of Pediococcus pentosaceae is a surface protein extracted from Pediococcus pentosaceae. Compared with the prior art, in the application of the present invention, the extraction method of Pediococcus pentosaceus surface protein has low investment, simple operation, and high antibacterial protein yield. The excellent antibacterial effect of bacteria provides new strategies and approaches for the potential application of the surface protein of Pediococcus pentosaceae in the field of antibacterial agents and antibacterial materials.

Description

technical field [0001] The invention belongs to the new application technical field of Pediococcus pentosaceae, and in particular relates to the application of a surface protein of Pediococcus pentosaceae in inhibiting food-borne pathogenic bacteria. Background technique [0002] Probiotics, also known as microecological regulators and live bacterial preparations, are a class of active microorganisms that can have beneficial effects on the host's physiological functions. They can regulate intestinal microecology, improve the intestinal environment, regulate intestinal flora, inhibit Pathogenic bacteria and other effects, mainly including bifidobacteria, lactic acid bacteria and yeast. Pediococcus pentosaceus belongs to the genus Pediococcus of the family Streptococcus and is a Gram-positive bacterium. It is a type of lactic acid bacteria because it can ferment glucose to produce lactic acid. It is widely distributed in traditional fermented foods such as pickles, cheese, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61P31/04C07K1/14C07K1/34A23L3/3463
CPCA61K38/02A61P31/04A23L3/3463C07K1/145C07K1/34
Inventor 饶胜其胡源杨振泉陈瑞孙艳菲王露江睿邓淳
Owner YANGZHOU UNIV
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