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Application of sipunculus nudus polysaccharide to preparation of drugs or health care products for prevention and treatment of osteoporosis (OP)

A technology for osteoporosis and health care products, applied in the direction of polysaccharide/gum-containing food ingredients, applications, drug combinations, etc., to achieve the effect of no toxic side effects and good application potential

Active Publication Date: 2019-03-12
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the anti-osteoporosis activity of sandworm polysaccharide

Method used

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  • Application of sipunculus nudus polysaccharide to preparation of drugs or health care products for prevention and treatment of osteoporosis (OP)
  • Application of sipunculus nudus polysaccharide to preparation of drugs or health care products for prevention and treatment of osteoporosis (OP)
  • Application of sipunculus nudus polysaccharide to preparation of drugs or health care products for prevention and treatment of osteoporosis (OP)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Effect of sandworm polysaccharide on the proliferation of MC3T3-E1 pre-osteoblasts cultured in vitro

[0021] 1. cells

[0022] Mouse preosteoblast MC3T3-E1 (purchased from Shanghai Cell Bank, Chinese Academy of Sciences). Cells were routinely cultured in α-MEM (Hyclone, USA) complete medium containing 10% fetal bovine serum (GIBCO, USA) in 5% CO 2 1. Cultivate in a 37°C incubator (Thermo), and when the cell confluence reaches 80%, digest with 0.25% trypsin (US sigma), and carry out subculture.

[0023] 2. MTT method to detect the proliferation of MC3T3-E1 pre-osteoblasts

[0024] Cells in the logarithmic growth phase were taken and resuspended in α-MEM medium containing 10% fetal bovine serum at a cell density of 5×10 4 individual / mL. Inoculated in three 96-well cell culture plates, 100 μL per well, after 24 hours, the cells adhered to the wall, removed the culture medium, washed once with phosphate buffered saline (PBS), and then added different concentr...

Embodiment 2

[0029] Example 2: Effect of sandworm polysaccharide on osteogenic differentiation of MC3T3-E1 cells cultured in vitro

[0030] 1. Alkaline phosphatase (ALP) activity detection

[0031] Pre-osteoblast MC3T3-E1 was inoculated into a 12-well cell plate, and the original culture medium was removed after 24 hours of cell adhesion, and then added with different concentrations of sandworm polysaccharide (0, 6.25, 12.5, 25, 50, 100mg / mL) respectively. α-MEM culture medium, a total of 6 groups, each with 3 replicate wells. Aspirate the cell culture medium on the 6th day, 2.5×10 3Centrifuge at r / min for 10 min, take the supernatant, and use the ALP activity detection kit (Nanjing Jiancheng) to measure the ALP activity. Prepare blank wells (buffer solution 50 μL, matrix solution 50 μL, double distilled water 30 μL), standard wells (buffer solution 50 μL, matrix solution 50 μL, 0.02 mg / mL phenol standard application solution 30 μL), assay wells (buffer solution 50 μL, matrix solution 50...

Embodiment 3

[0035] Example 3: Effect of sandworm polysaccharide on bone mineral density of 10-month-old mice

[0036] 1. Grouping of experimental animals:

[0037] Forty 8-month-old KM mice (purchased from Guangdong Provincial Medical Experimental Animal Center) were adaptively fed for 2 weeks and randomly divided into 4 groups with 10 mice in each group. Group A is the blank control (CON) group, and the mice in this group are given normal saline 10mL / (kg / d) by intragastric administration every day; Group B is the low-dose group of polysaccharides, and the mice in this group are given 25mg polysaccharides by intragastric administration every day / (kg / d); In the group C group of sandworm polysaccharide middle dose group, the mice of this group were given 50 mg / (kg / d) of sandworm polysaccharide by intragastric administration every day; Stomach administration of sandworm polysaccharide 100mg / (kg / d). The mice in the 4 groups were given free access to water and food. The experiment was admi...

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Abstract

The invention discloses application of sipunculus nudus polysaccharide to preparation of drugs or health care products for prevention and treatment of osteoporosis (OP). Pharmacological experiment research proves that the sipunculus nudus polysaccharide can significantly improve the proliferative activity of preosteoblast MC3T3-E1 cultured in vitro, promotes the preosteoblast MC3T3-E1 to be subjected to differentiation, and can significantly increase the bone mineral density of 10-month years old of mice; and the results show that the sipunculus nudus polysaccharide has the potential of treating or preventing OP. According to the novel purpose of the sipunculus nudus polysaccharide, the indication or the application range of the sipunculus nudus polysaccharide is enlarged, and the sipunculus nudus polysaccharide is expected to be developed into the new drugs or health care products for prevention and treatment of OP.

Description

technical field [0001] The invention relates to the technical field of application of natural products, in particular to the application of polysaccharides from marine products in medicine or health care products. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a systemic, systemic bone disease characterized by decreased bone mass and damage to the microstructure of bone tissue, resulting in increased bone fragility and increased risk of fracture. At the same time, OP is caused by bone aging. degenerative disease. Epidemiological surveys show that women are significantly higher than men, and with the acceleration of the aging process of our society, the incidence rate is on the rise. As of 2013, China's elderly population has exceeded 200 million, and it is estimated that by 2020, the number of osteoporosis patients in my country will reach 286.6 million. It is estimated that by the 1950s, the number of patients with hip fractures caused by osteoporosis wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P19/10A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P19/10A23V2200/306A23V2250/51
Inventor 李立吕应年刘义吴科锋
Owner GUANGDONG MEDICAL UNIV
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