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An integrated extraction method of multiple bioactive components in garlic

A bioactive component and extraction method technology, which is applied in the field of integrated extraction of various bioactive components in garlic, can solve the problems of poor taste, color and mouthfeel, unseparated extraction of bioactive components, and high production costs, so as to reduce the production cost. Pollution, less loss of active ingredients, and the effect of reducing the cost of raw materials

Active Publication Date: 2021-11-09
大连平岛天然产物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few integrated extraction methods for active substances in garlic in China, and there are also the following problems: the comprehensive utilization process of garlic introduced in CN1087597C is single, and no further separation and extraction of various biological active components is carried out; the comprehensive utilization process of garlic introduced in CN103598544B The enzymatic hydrolysis preparation method is a three-step enzymatic hydrolysis method, which is cumbersome, time-consuming, high production cost, and the important active ingredient SOD has not been separated; garlic protein peptide products have not undergone deodorization and decolorization processes, and the products may have taste and color And the defect of poor taste

Method used

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  • An integrated extraction method of multiple bioactive components in garlic

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preparation example Construction

[0029] S3. Preparation of SOD: the liquid phase obtained in the step S2 is passed through a ceramic membrane with a pore size of 20000Da (the first membrane separation), the permeate is used for standby, the retentate is collected, separated by acetone precipitation and freeze-dried to obtain SOD;

[0030] S4. Preparation of garlic polysaccharide: the permeate obtained by membrane separation for the first time in the step S3 is passed through an organic membrane with a pore size of 5000Da (membrane separation for the second time), and the permeate is used for subsequent use, and the retentate is collected for decolorization, Garlic polysaccharide was obtained by freeze-drying after concentration under reduced pressure;

[0031] S5. Preparation of alliin: the permeate obtained from the second membrane separation in the step S4 is concentrated under reduced pressure to a solid content of 15% to 30%, then dissolved in water after precipitation and separation, and recrystallized fr...

Embodiment 1

[0045] Garlic pretreatment: 100kg of garlic is peeled and washed to obtain about 91kg of garlic cloves, and the allinase is inactivated by heat treatment at 60°C for 20 minutes. Then, it was treated in a 4°C operating room, crushed the garlic cloves, added 100L of 0.5M NaCl solution pre-refrigerated at 4°C, used colloidal grinding, high-pressure homogeneous and wall-breaking treatment, and then added 0.5M NaCl solution to salt out the protein, etc. Precipitate until the solid content is 10%, and extract with stirring for 12 hours to obtain a mixed extract liquid. The mixed extraction feed liquid was separated by a decanter separator to obtain 150 L of liquid phase and 30 kg of solid phase enzymatic hydrolysis substrate.

[0046] Preparation of SOD: pass the 150L liquid phase obtained in the pretreatment step through a ceramic membrane with a pore size of 20,000Da (the first membrane separation), keep the permeate 120L for later use, collect about 30L of the retentate, add 30L ...

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Abstract

The invention discloses an integrated extraction method of various bioactive components in garlic, comprising steps: S1. garlic pretreatment; S2. first solid-liquid separation: decanter separator to obtain liquid phase and solid phase enzymatic hydrolysis bottom SOD; S3. Preparation of SOD: extraction of retentate by liquid phase first membrane separation, refining SOD; S4. Preparation of garlic polysaccharide: extraction of retentate by second membrane separation, refinement of garlic polysaccharide; S5. Preparation of alliin: The second membrane separation permeate extracts and refines alliin; S6. Enzymolysis process: the solid-phase enzymolysis substrate is prepared by adding water and then enzymatically hydrolyzed with a compound protease to inactivate the enzyme to obtain the enzymolysis product; S7. The second solid-phase enzymolysis Liquid separation: decanter separator to obtain garlic oligopeptide liquid and solid phase; S8. Preparation of odorless garlic powder: solid phase to obtain odorless garlic powder; S9. Preparation of garlic oligopeptide: garlic oligopeptide liquid Concentrated and refined garlic oligopeptide. The invention can make full use of garlic resources and avoid waste of resources.

Description

technical field [0001] The invention relates to the field of biochemical enzymatic hydrolysis extraction, and more specifically relates to an integrated extraction method of various bioactive components in garlic. Background technique [0002] Garlic (garlic), also known as gourd or Kang herb, belongs to the bulb of the Liliaceae Allium plant garlic, and its cultivation history has been at least 2,000 years. Garlic is considered to be one of the best daily health foods. Regular consumption has the effects of dispelling dampness, resisting toxins, strengthening the spleen and strengthening the body. Modern medical research shows that garlic has many functions such as antibacterial and anti-inflammatory, improving immunity, preventing and treating cardiovascular diseases, and preventing and treating tumors. These health care and pharmacological functions are related to the functional components contained in it. [0003] Among them, alliin is a non-protein sulfur-containing am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12P21/06C08B37/00C07C315/06C07C317/48
CPCC07C315/06C08B37/00C08B37/0003C12N9/0089C12P21/06C12Y115/01001C07C317/48
Inventor 黄祥贤续彦龙
Owner 大连平岛天然产物科技有限公司
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