Method for integrated extraction of plurality of biologically active ingredients in soft-shelled turtle

Abstract

The invention discloses a method for integrated extraction of a plurality of biologically active ingredients in a soft-shelled turtle. The method comprises the following steps: S1, adding a complex protease A into a soft-shelled turtle crushed material liquid for enzymolysis; S2, filtering the soft-shelled turtle enzymolysis product, separating soft-shelled turtle crushed bones and a soft-shelledturtle meat liquid product, carrying out centrifugation to obtain a water-phase product of soft-shelled turtle meat and an oil-phase product of the soft-shelled turtle meat; S3, removing the fishy smell of the water-phase product of soft-shelled turtle meat, and carrying out decoloration, fine filtering, concentration and drying to obtain the soft-shelled turtle meat oligopeptide powder; S4, steaming a soft-shelled turtle crushed bone liquid, carrying out filtering to obtain a bone residue and a crushed bone liquid product, carrying out centrifugation to obtain a protein solution of soft-shelled turtle bones and an oil-phase product of the soft-shelled turtle bones; S5, adding a complex protease B into the protein solution of the soft-shelled turtle bones for enzymolysis to obtain a soft-shelled turtle bone protein enzymolysis product; S6, removing the fishy smell of the soft-shelled turtle bone protein enzymolysis product, and carrying out decoloration, fine filtering, concentration and drying to obtain oligopeptide powder of the soft-shelled turtle bone protein; S7, treating the bone residue to obtain soft-shelled turtle shell powder; and S8, mixing the oil-phase product of the soft-shelled turtle meat and the oil-phase product of the soft-shelled turtle bones to obtain soft-shelled turtle oil. According to the method provided by the invention, the soft-shelled turtle resources can be fully utilized, and resource waste is avoided.

Description

technical field [0001] The invention relates to the field of biochemical enzymatic hydrolysis extraction, and more specifically relates to a method for integrated extraction of multiple biologically active components in soft-shelled turtles. Background technique [0002] Soft-shelled turtle is also known as soft-shelled turtle, turtle, foot fish, water fish, chordate phylum, vertebrate sub-phylum, reptile class, turtle order, turtle family, soft-shelled turtle genus, and is a precious aquatic economic animal. [0003] Soft-shelled turtle meat is tender and delicious, rich in protein, thiamine, riboflavin, niacin, lecithin and other nutrients. Among them, the amino acids in the protein are complete, high in content, and the ratio is relatively reasonable. The essential amino acids are especially important for human body. The content of the main restrictive amino acid lysine and the flavored glutamic acid is significantly higher than that of other foods; the meat is rich in vi...

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to overcome the defect of low utilization rate of soft-shelled turtle in the prior art, provide a kind of method for integrated extraction of multiple bioactive components in soft-shelled turtle, through the integrated processing of soft-shelled turtle, fully extract soft-shelled turtle meat oligopeptide, Soft-shelled turtle bone protein oligopeptide, soft-shelled turtle powder and soft-shelled turtle oil can improve the utilization rate of soft-shelled turtles and avoid waste of resources

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