Device and method for detecting circular dichroism

Abstract

The invention relates to a device and a method for detecting circular dichroism. A laser having a wavelength in a visible light region is modulated into left circularly polarized light and right circularly polarized light, so that the modulated chiral light passes through transparent microspheres having a super-resolution imaging capability to irradiate a chiral molecular film; The focusing capacity of the transparent microspheres to the light increases an absorption difference between the left circularly polarized light and the right circularly polarized light by chiral molecules and is detected by a lock-in amplifier, thereby achieving an aim of reducing a detection limit of the circular dichroism; and meanwhile, the super-resolution capability of the transparent microspheres is used toidentify adjacent chiral molecular clusters, which can detect chiral substances more effectively. Compared with a conventional technology, the device and the method can realize fast, accurate and non-invasive detection of the chiral molecules and enhance detection signals, and have the advantages of convenience in operation, low sample consumption, no contact damage to biological samples and maintenance of original biological characteristics of the chiral substances.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a device and method for detecting circular dichroism. Background technique [0002] Chirality is a concept in geometry, which means hand. As early as the middle of the 19th century, chemists discovered that the solution of crystals can convert linearly polarized light into left circularly polarized light and right circularly polarized light respectively, which is the so-called optical rotation phenomenon. The definition of a chiral molecule is that a molecule does not overlap with its mirror image, then both it and its mirror image are chiral molecules and are enantiomers of each other. People usually mark chiral molecules as L-(levo, left-handed) conformation and D-(Dextro, right-handed) conformation, or S (sinister, left-handed) and R (rectus, right-handed) conformation. Chiral molecules are one of the elements of life. Many biological chiral molecules such as pr...

AI Technical Summary

Problems solved by technology

But usually the circular dichroism signal is relatively weak, and the signal of small or low-concentration chiral molecules may be masked, such as non-invasive, low concentration and other detection conditions, so the method of improving the circular dichroism signal has been pursued by researchers

Moreover, when performing circular dichroism detection on chiral molecules, most of the samples are solutions, and the test takes a long time, which cannot be widely used in the research of low-concentration molecular film detection and real-time observation and identification of adjacent chiral molecular particles.

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