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Polydopamine nano diagnosis and treatment agent and preparation method thereof

A polydopamine nano and dopamine nano technology, applied in the field of biomedical materials, can solve the problems of oxygen consumption, aggravating tumor tissue hypoxia, unfavorable photodynamic therapy, etc., to promote uptake, improve catalytic activity, and improve tumor microenvironment hypoxia. Effect

Active Publication Date: 2019-02-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with normal tissues, tumors are in a hypoxic environment, which is not conducive to the effective implementation of photodynamic therapy, and the treatment process will consume oxygen, thereby aggravating the hypoxia in tumor tissues.

Method used

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  • Polydopamine nano diagnosis and treatment agent and preparation method thereof
  • Polydopamine nano diagnosis and treatment agent and preparation method thereof
  • Polydopamine nano diagnosis and treatment agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: Preparation of Rh@MPDA nanoparticles

[0040] (1) Preparation of MPDA nanoparticles:

[0041] The carrier mesoporous polydopamine MPDA is prepared from the reference literature, and the particle size is about 200nm.

[0042] Weigh 0.15g of dopamine hydrochloride and 0.1g of F-127, dissolve them in 10mL of ethanol and ultrapure water 1:1 system, stir for 20min to fully dissolve and mix. In an ultrasonic water bath, slowly add 160 μL of TMB, ultrasonic for 3 minutes, the solution becomes milky white visible to the naked eye. Then add 375 μL of ammonia water, the solution turns dark brown, stir on a magnetic stirrer for 2 hours, and centrifuge for 10 minutes (13000 rpm / min, 4°C) to collect the precipitate. Wash once with ethanol, wash twice with ultrapure water, and finally resuspend with ultrapure water to obtain the mesoporous polydopamine solution.

[0043] (2) Preparation of Rh@MPDA nanoparticles:

[0044] Centrifuge 10 mL of 1 mg / mL mesoporous polydo...

Embodiment 2

[0047] Embodiment 2: Preparation of Rh@MPDA nanoparticles

[0048] (1) Same as Step 1 of Example 1;

[0049] (2) Same as Step 2 of Example 1, except that 1 mL of 10 mg / mL rhodium trichloride hydrate solution was added.

[0050] (3) Transmission electron microscope observation:

[0051] After fully ultrasonically dispersing the Rh@MPDA nanoparticles prepared above, they were added dropwise onto the copper grid, dried overnight in the air at room temperature to collect the copper grid, and observed with a transmission electron microscope. The transmission electron microscope picture is as follows: image 3 shown. As can be seen from the figure, the distribution of rhodium nanoparticles in the above-mentioned Rh@MPDA is relative to that of Example 1 ( figure 2 ) is denser, indicating that increasing the dosage of rhodium precursors can effectively increase the loading of rhodium nanoparticles.

Embodiment 3

[0052] Embodiment 3: Preparation of Rh@MPDA nanoparticles

[0053] (1) Same as Step 1 of Example 1;

[0054] (2) Same as Step 2 of Example 1, except that 2 mL of 10 mg / mL rhodium trichloride hydrate solution was added.

[0055] (3) Transmission electron microscope observation:

[0056] After fully ultrasonically dispersing the Rh@MPDA nanoparticles prepared above, they were added dropwise onto the copper grid, dried overnight in the air at room temperature to collect the copper grid, and observed with a transmission electron microscope. The transmission electron microscope picture is as follows: Figure 4 shown. As can be seen from the figure, the content of rhodium loaded in the above-mentioned Rh@MPDA is relative to that of Example 1 ( figure 2 ) and Example 2 ( image 3 ) are more, and the excess rhodium nanoparticles are aggregated and distributed on the surface of the carrier, which further shows that increasing the dosage of rhodium precursors can effectively increa...

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Abstract

The invention discloses a polydopamine nano diagnosis and treatment preparation and a preparation method thereof. The nano diagnosis and treatment agent is prepared by the following steps: taking mesoporous polydopamine as a carrier, respectively supporting rhodium nanoparticles in the hole and on the surface of the mesoporous polydopamine by virtue of a hydrothermal synthesis reaction, and adsorbing a photosensitizer dihydroporphin (Ce6) onto the polydopamine and rhodium nanoparticles, thereby obtaining the polydopamine nano diagnosis and treatment agent with excellent dispersion property. Byutilizing photothermal effects of the carrier mesoporous polydopamine and the rhodium nanoparticles, the temperature of the tumor site is locally raised. By combining with catalytic characteristics of the rhodium nanoparticles, hydrogen peroxide in a tumor microenvironment is catalyzed to decompose so as to produce oxygen, and aims of producing singlet oxygen and effectively killing cancer cellsare achieved under the conditions of the photosensitizer Ce6 and applied laser. Meanwhile, by combining with photoacoustic imaging characteristics of the polydopamine, the aim of guiding photothermaltherapy and photodynamic synergistic therapy by photoacoustic imaging can be achieved, and the tumor treatment effect is expected to be improved. Moreover, the polydopamine nano diagnosis and treatment agent is excellent in biocompatibility, and has clinical application potential.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a polydopamine nanometer diagnosis and treatment agent and a preparation method thereof. Background technique [0002] Cancer is a major disease that threatens human life. The current common radiotherapy, chemotherapy and surgery have their own limitations, so that the treatment effect of cancer is not ideal. Therefore, a large number of researchers focus on the integration of diagnosis and treatment, use the advantages of nanostructures to concentrate the functions of diagnosis and treatment into nanoparticles, use the EPR effect to target tumor sites, and realize the diagnosis and treatment of cancer at the same time. [0003] Photothermal therapy technology, as a new type of treatment strategy, has received high attention in the field of cancer diagnosis and treatment in recent years. Photothermal therapy based on nanomaterials uses near-infrared light with strong tissue ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/34A61K47/69A61K49/22A61P35/00
CPCA61K41/0052A61K41/0071A61K47/34A61K49/22A61K47/6949A61P35/00A61K2300/00
Inventor 曹众丁梦丽
Owner SUN YAT SEN UNIV
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