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Method for detecting contents of glucose in cell culture mediums by utilizing Raman spectroscopy

A Raman spectrum detection and Raman spectrometer technology, which is applied in the detection field, can solve problems such as the inability to realize quantitative detection of samples, and achieve the effect of avoiding consumption and eliminating interference factors.

Inactive Publication Date: 2019-02-15
CHINA ANIMAL HUSBANDRY IND
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Problems solved by technology

[0005] Although Raman spectroscopy detection has been applied in many fields, for objects with complex components like cell culture medium, it is not yet possible to achieve a completely non-destructive quantitative detection of the sample

Method used

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  • Method for detecting contents of glucose in cell culture mediums by utilizing Raman spectroscopy
  • Method for detecting contents of glucose in cell culture mediums by utilizing Raman spectroscopy
  • Method for detecting contents of glucose in cell culture mediums by utilizing Raman spectroscopy

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Embodiment 1

[0030] Example 1: Establishment of Raman Spectroscopy Quantitative Determination of Glucose Standard Model

[0031] (1) Spectrum adjustment: fixed by the cuvette bracket, adjust the distance adjustment sleeve of the cuvette bracket to obtain the best working distance of 2 mm for laser projection;

[0032] (2) Accurately configure the standard glucose concentration solution as follows: glucose concentrations of different concentrations from 0.1g / L to 320g / L, specifically: 0.1g / L, 0.5g / L, 1g / L, 2g / L, 5g / L, 10g / L, 15g / L, 20g / L, 25g / L, 30g / L, 35g / L, 40g / L, 45g / L, 50g / L, 55g / L, 60g / L, 65g / L L, 70g / L, 75g / L, 80g / L, 85g / L, 90g / L, 95g / L, 100g / L, 110g / L, 120g / L, 130g / L, 140g / L, 150g / L, 160g / L, 150g / L, 160g / L, 170g / L, 180g / L, 190g / L, 200g / L, 210g / L, 220g / L, 230g / L, 240g / L, 250g / L, 260g / L L, 270g / L, 280g / L, 290g / L, 300g / L, 310g / L, 320g / L.

[0033] Each standard solution sample was loaded into a cuvette for determination.

[0034] (3) Construct and debug the Raman spectrum standard mo...

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Abstract

The invention provides a method for detecting contents of glucose in cell culture mediums by utilizing Raman spectroscopy. The method comprises the following steps of: 1) preparing a series of standard glucose solution with a concentration from 0.1 g / L to 350 g / L by using glucose, placing the standard glucose solution in a cuvette of a Raman spectrometer, setting a laser projection distance as 1-2mm and acquiring spectral signals by utilizing the Raman spectrometer; and 2) establishing a standard model according to characteristic peaks and concentrations of Raman spectrums of the standard glucose solution with different concentrations. The method for detecting contents of glucose in cell culture mediums by utilizing Raman spectroscopy is applied to the field of veterinary cell culture medium detection, and does not have hemoglobin interference factors; and at the same time, the interference factors are eliminated through adjusting Raman spectral distances and wavelength time. Comparedwith the existing glucose solution detection technology in the field of veterinary biological products, the method has the characteristics of being fast, simple, accurate and sensitive.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for detecting glucose content by Raman spectrum. Background technique [0002] At present, in the field of veterinary biological products, the method for detecting the glucose content in samples such as culture medium is mainly to use instruments and equipment such as blood glucose meters or biochemical analyzers for sampling and detection. Glucose is an important carbon source in the growth of microorganisms, but its concentration must be controlled. When glucose exists in the medium, the ability of microorganisms to utilize lactose is inhibited. Therefore, the glucose content in the medium needs real-time quantitative detection. However, the existing glucose detection methods have disadvantages such as cumbersome operation, high consumption of consumables, and long time consumption. [0003] Raman spectroscopy is a type of scattering spectroscopy that o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 杨小蓉马良许磊李晓冉
Owner CHINA ANIMAL HUSBANDRY IND
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