A kind of rapid cultivation method of fungi and fungi-cyanobacteria compound lichen crust

A cultivation method and technology of cyanobacteria, applied in the field of environmental biology, can solve the problems of high maintenance costs, moss materials not suitable for large-scale production, and desertification soil restoration, etc., achieve short cultivation period, increase carbon and nitrogen content and soil enzyme activity , Improve the effect of physical and chemical properties

Inactive Publication Date: 2021-07-30
WUHAN UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Chinese Patent (Application) No. 200510018149 provides a method for improving desert and semi-desert soil by soil algae; Chinese Patent (Application) No. 201510522936.5 provides an inoculation method for desert algae artificial biological crust and sand fixation; Chinese Patent (Application) No. 201410691489.1 provides a method for rapid cultivation of moss crusts on sandy land; Chinese Patent (Application) No. 201610899794 provides a method for cultivating composite moss and desert algae crusts; the above In the invention, cyanobacteria or moss are used to cultivate artificial biological crusts, but there is still a large room for improvement due to the drought resistance of a single cyanobacteria crust, and the moss materials required for the cultivation of moss crusts are not suitable for large-scale production, and during the cultivation process High maintenance costs, so not suitable for large-scale desertification soil remediation

Method used

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  • A kind of rapid cultivation method of fungi and fungi-cyanobacteria compound lichen crust
  • A kind of rapid cultivation method of fungi and fungi-cyanobacteria compound lichen crust
  • A kind of rapid cultivation method of fungi and fungi-cyanobacteria compound lichen crust

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening of high-yielding exopolysaccharide strains and identification of fermentation products

[0034] Collect the complete lichen crusts that grow naturally in arid areas, weigh 10g, put them in a 250mL Erlenmeyer flask filled with 90mL sterile water, vibrate at 30°C for 20min at 150r / min, and let stand for 10min to obtain a soil suspension. Dilute the bacterial suspension to 10 –6 After that, take 10 of them -4 , 10 -5 , 10 -6 200 μL of the solution under 3 dilution gradients, spread on the PDA medium, and culture it upside down in a constant temperature incubator at 30°C for 48 hours, select different types of typical single colonies, and after repeated purification on the plate, store them in the PDA slant medium at 4°C stand-by.

[0035] Add 100mL sterilized liquid PDA medium (containing sterile glass beads) to a 250mL Erlenmeyer flask, inoculate each bacterial strain stored on the PDA slant medium into the PDA medium, and shake at 150rpm for 3 da...

Embodiment 2

[0044] The soil fertility comparison of embodiment 2 fungus-cyanobacteria compound crust and single algal crust

[0045] Inoculate the outer bottle mold Zh2 into the PDA medium, shake and culture at 150rpm for 3 days, and wait until it reaches the logarithmic growth phase, and set aside; Depository Center (FACHB-886). The algae is isolated from the biological soil crust in Shapotou, Ningxia. It is an excellent algae used for sand fixation in the Kubuqi Desert. The algae is inoculated in sterile BG 110 culture medium, placed in a light incubator with a light intensity of 50 μE / (m 2 s), 25±1°C, continuous light, and aeration to the logarithmic phase.

[0046] Cultivate the exophyll mold Zh2 and Algae spp. to the logarithmic phase, determine the biomass by blood counting and colorimetry, mix after dilution, so that the mixed culture contains 5-10 μg (chlorophyll a) / mL cyanobacteria and 10 6 ~10 8 CFU / mL outer bottle mold Zh2. With 1±0.3μg (chlorophyll a) / cm 2 The amount of ...

Embodiment 3

[0050] Example 3 Comparison of the growth curves of fungi-cyanobacteria composite crusts and single cyanobacteria crusts under different drought stresses

[0051] Different soil moisture content groups (0, 5%, 10%, 15% and 20%) are set, and the fungus-cyanobacteria mixture and single cyanobacteria are respectively inoculated on the quicksand surface, and the inoculation method is the same as that of embodiment 3. Continuous light and constant temperature cultivation, during which water should be replenished according to the soil water content at 9:00 every day. Every 5 days during the culture period, take the crust per unit area into a 10mL centrifuge tube, add 5mL of 95% ethanol, vortex and mix evenly, then place in a refrigerator at 4°C for extraction for 24h, shake at least 3 times during the period, and centrifuge at 8000g for 10min after the extraction. The absorbance was measured at 665nm and 649nm, and the content of chlorophyll a was calculated. The change curve of cr...

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Abstract

The invention belongs to the technical field of environmental biology, and in particular relates to a rapid cultivation method of fungi and fungi-cyanobacteria compound lichen crusts. The exophyla mold Zh2 screened in the present invention belongs to high-yield exopolysaccharide strains, and the exophyla mold Zh2 is compounded with cyanobacteria and applied to the artificial sand fixation of biological crusts. On the one hand, the exophyla mold Zh2 can provide a carbon source for the growth of cyanobacteria, and on the other hand On the one hand, it can cement the sand particles to form stable aggregates, increase the carbon and nitrogen content of the soil and soil enzyme activity, improve the physical and chemical properties of the soil, and significantly promote the nutrient level of the soil. The invention mixes fungi and blue-green algae and inoculates quicksand, can quickly form stable composite crusts, has a short cultivation period, improves the stability of artificial crusts, and accelerates the succession process from algae crusts to lichen crusts. The fungi-cyanobacteria composite lichen crust cultivated in the present invention has significantly higher drought resistance than the single algal crust, indicating that the composite crust can better adapt to the harsh environment of arid regions.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a rapid cultivation method of fungi and fungi-cyanobacteria composite lichen crusts. Background technique [0002] Soil desertification is one of the most important environmental problems facing our country at present. According to the fifth national desertification and sandification monitoring results, as of 2014, the national desertification land area was 2.6116 million square kilometers, accounting for 27.2% of the national land area; the desertification land area was 1.721 million square kilometers, accounting for 17.9% of the national land area. The problem needs to strengthen desertification control. At present, my country's desertification prevention and control measures include physical methods, chemical methods and biological methods, among which bioremediation is recognized as an efficient, cheap and environmentally friendly way, and has bette...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20C09K17/14C09K105/00C12R1/645
CPCC09K17/14C09K2105/00C12N1/14C12N1/20C12N1/145C12R2001/645
Inventor 陈兰洲周香君张渝蕊叶超然柯檀李双喜
Owner WUHAN UNIV
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