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Corn stalk-soybean meal mixed fermented material, preparation method thereof and application thereof

A corn stalk, mixed fermentation technology, applied in the direction of bacteria, application, animal feed, etc. used in food preparation, can solve the problems of polluting the environment, loss of economic value of straw, affecting a harmonious society, etc. Intestinal digestive function, the effect of improving growth performance

Active Publication Date: 2019-02-01
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional planting habits usually return a large amount of crop straw directly to the field, discard it, or even burn it, which not only pollutes the environment, affects the construction of a harmonious society, but also loses the economic value of the straw

Method used

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  • Corn stalk-soybean meal mixed fermented material, preparation method thereof and application thereof
  • Corn stalk-soybean meal mixed fermented material, preparation method thereof and application thereof
  • Corn stalk-soybean meal mixed fermented material, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Zizhi is Ganoderma sinense (Ganoderma sinensis), the number is BNCC 143276, Nachuanglian Biotechnology Co., Ltd.; it is prepared into spore powder by fermentation.

[0035] Activation of Aspergillus oryzae and Aspergillus niger and preparation of fermentation strains

[0036] Aspergillus oryzae BNCC195382, Beina Chuanglian Biotechnology Co., Ltd.;

[0037] Aspergillus niger CICC2377, China Industrial Microorganism Culture Collection Management Center;

[0038] Crush soybean shells and pass through a 10-mesh sieve, sterilize at 121°C for 15 minutes; adjust the water content to 50-65%, and inoculate the aspergillus oryzae and aspergillus niger strains activated by the bean juice slant medium in the bean Shell culture medium, cultivated at 25-30°C for 48-60 hours, to obtain fermented strains with a content of ≥10 9 cfu·g -1 .

Embodiment 2

[0040] Lactobacillus salivarius is Lactobacillus salivarius (Lactobacillus salivarius) XJP2, and the preservation number is CGMCCNO:11386.

[0041] Activation and fermentation medium: MRS medium: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, glucose 20g / L, Tween 801ml / L, dipotassium hydrogen phosphate 2g / L, sodium acetate 5g / L L, triammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, pH6.2.

[0042] Inoculate the preserved Lactobacillus salivarius XJP2 on the MRS solid medium for activation culture, and inoculate the activated strains on the MRS liquid medium with an inoculation amount of 1%, and cultivate them at 37°C and 160rpm for 16-24h until the bacterial content is ≥10 9 cfu / mL.

[0043] Lactobacillus plantarum CICC21790, China Industrial Microorganism Culture Collection Management Center;

[0044] Activation and fermentation medium: MRS medium: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, glucose 20g / L, Tween 801ml / L, dipotass...

Embodiment 3

[0053] A preparation method for fermented corn stalks, the steps are:

[0054] Crush the corn stalks through a 10-mesh sieve, sterilize with high pressure steam at 121°C for 30 minutes, and cool to room temperature after the sterilization is completed; spray the compound solution containing Zizhi spores on the crushed corn stalks; until the water content of the corn stalks is 45-55 %, 25°C, fermented for 3 days;

[0055] The compound solution containing Zizhi spores is: glucose 20g / L, KH 2 PO 4 3g / L, MgSO 4 0.7g / L, vitamin B 1 0.01g / L, Zizhi spores 5×10 7 pc / L, mannitol 15g / L, water balance.

[0056] 1. Degradation of corn straw by different fermentation bacteria

[0057] 1.1 Test strains

[0058] Ganoderma lucidum (Purple lucidum) (Ganoderma sinense), No. BNCC 143276, Nachuanglian Biotechnology Co., Ltd.;

[0059] The number of Trametes versicolor is BNCC145690, Beina Chuanglian Biotechnology Co., Ltd.;

[0060] Aspergillus nige (Aspergillus nige) number is CICC2377,...

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PUM

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Abstract

The present invention discloses a corn stalk-soybean meal mixed fermented material, a preparation method thereof and an application thereof, and belongs to the technical field of biological fermentation. The corn stalk-soybean meal mixed fermented material is prepared by mixing fermented corn stalks, corn flour and fermented soybean meal according to weight ratios of (1-6):(0.5-3):(2-12), and conducting enzymatic hydrolysis and secondary fermentation. The fermented corn stalks are prepared by solid fermentation by ganoderma sinense. The prepared corn stalk-soybean meal mixed fermented materialis high in nutrient content, can improve growth performance of animals, can also improve gastrointestinal digestion functions, improves body's antioxidant performance and enhances immunity.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a corn stalk-soybean meal mixed fermentation material, a preparation method and application thereof. Background technique [0002] Crop straw is an auxiliary product of agricultural production, which has not been fully and effectively utilized in our country. Traditional planting habits usually return a large amount of crop straw directly to the field, discard it, or even burn it, which not only pollutes the environment and affects the construction of a harmonious society, but also causes the economic value of the straw to be lost. [0003] Corn stalks contain more than 30% carbohydrates, 2%-4% protein and 0.5%-1% fat; among them, the content of ineffective crude fiber is high, the content of crude protein is low, and the nutrition is low. It needs to be treated before feeding animal. [0004] Soybean cake is a plant-based protein material with high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/37A23K10/14A23K10/12A23K20/105A23K20/163A23K20/174A23K20/26
CPCA23K10/12A23K10/14A23K10/30A23K10/37A23K20/105A23K20/163A23K20/174A23K20/26A23V2400/181A23V2400/169Y02P60/87
Inventor 杨庆利任瑶瑶赵海燕朱英莲唐娟于春娣
Owner QINGDAO AGRI UNIV
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