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Method for synchronously extracting silybum marianum oil and hydrolyzed protein

A technology for hydrolyzing protein and extracting water, applied in the biological field, can solve the problems of protein variability, low oil yield, poor oil quality, etc., and achieve the effects of low extraction temperature, high oil extraction rate, and reduced production cost.

Inactive Publication Date: 2019-01-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of low oil yield, poor oil quality, and protein variability in existing pressing methods, the present invention aims to solve the above problems;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Select qualified milk thistle seeds, use mechanical crushing, and the crushed material is passed through a 60-mesh sieve; the crushed milk thistle powder and water are mixed evenly with a mass ratio of 1:3, and the mixture of papain and amylase is added. Compound enzyme, the mass ratio of compound enzyme to milk thistle powder is 0.005:1; the mass ratio of papain to amylase is 1:1, under the conditions of pH 6.5 and temperature 35°C, stirring and enzymolysis for 1 hour, enzyme The solution mixture was inactivated at a temperature of 95 °C for 10 min;

[0027] The enzymolysis mixture was centrifuged at 4000 r / min for 15 min. After centrifugation, the first layer of clear oil, the second layer of emulsion, the third layer of protein hydrolyzate and the fourth layer of residue were obtained, and the clear oil was obtained by absorbing layer by layer. , emulsion and protein hydrolyzate. The emulsion was added petroleum ether at a volume ratio of 1:3 to extract milk thistle...

Embodiment 2

[0029] Select milk thistle seeds with qualified quality, and adopt mechanical crushing, and the crushed material is passed through a 60-mesh sieve. Mix the pulverized milk thistle powder and water evenly at a mass ratio of 1:5, add a compound enzyme of papain and amylase, the mass ratio of the compound enzyme to milk thistle powder is 0.02:1; papain and starch The mass ratio of the enzyme is 1:1. Under the conditions of pH 6.8 and temperature 45°C, stir the enzymolysis for 2 hours, and heat the enzymolysis mixture at 95°C for 10 minutes;

[0030] The enzymolysis mixture was centrifuged at 4000 r / min for 15 min. After centrifugation, the first layer of clear oil, the second layer of emulsion, the third layer of protein hydrolyzate and the fourth layer of residue were obtained, and the clear oil was obtained by absorbing layer by layer. , emulsion and protein hydrolyzate. The emulsion was added petroleum ether at a volume ratio of 1:3 to extract milk thistle oil, and then combi...

Embodiment 3

[0032] Select milk thistle seeds with qualified quality, and adopt mechanical crushing, and the crushed material is passed through a 60-mesh sieve. Mix the pulverized milk thistle powder and water evenly at a mass ratio of 1:7, add a compound enzyme of papain and amylase, the mass ratio of the compound enzyme to milk thistle powder is 0.035:1, papain and starch The mass ratio of the enzyme is 1:1. Under the conditions of pH 7.2 and temperature 55°C, stir the enzymolysis for 3 hours, and heat the enzymolysis mixture at 95°C for 10 minutes;

[0033] The enzymolysis mixture was centrifuged at 4000 r / min for 15 min. After centrifugation, the first layer of clear oil, the second layer of emulsion, the third layer of protein hydrolyzate and the fourth layer of residue were obtained, and the clear oil was obtained by absorbing layer by layer. , emulsion and protein hydrolyzate. The emulsion was added petroleum ether at a volume ratio of 1:3 to extract milk thistle oil, and then comb...

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Abstract

The invention discloses a method for synchronously extracting silybum marianum oil and hydrolyzed protein and belongs to the technical field of biological processing. The method comprises the following steps: taking silybum marianum seeds as raw materials; carrying out crushing, enzymolysis and carrying out high-temperature enzyme killing; centrifuging and separating an oil-water mixture obtainedby the enzymolysis; then preparing the silybum marianum oil and hydrolyzed protein powder respectively. The method disclosed by the invention has the advantages of moderate processing conditions, lowextraction temperature, high oil yield and uneasiness of denaturing the protein; two high-quality products including the silybum marianum oil and the hydrolyzed protein can be synchronously obtained;the prepared hydrolyzed protein can be used as a nutrient enhancing agent or a material of a health-care food, and has remarkable economic and social benefits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for simultaneously extracting milk thistle oil and hydrolyzed protein. Background technique [0002] Milk thistle is an herb of Compositae, native to the Mediterranean coast, and now widely distributed in Europe, North America, Asia, Africa and other places. It is cultivated in Shaanxi, Heilongjiang, Liaoning, Jiangsu, Hubei and other places in my country. Studies have shown that the oil content of milk thistle seeds is about 20% to 30%, which mainly contains oleic acid and linoleic acid, and the total unsaturated fatty acids are as high as 80%. Milk thistle oil also contains sterols such as β-sitosterol and campesterol, and is rich in vitamin E, which has high edible value and nutritional value. Milk thistle oil also has lipid-lowering, anti-oxidation and anti-arterial hardening etc. The protein content in milk thistle seeds is also very high. Studies have proved that mil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B1/04C11B3/00A23J1/14
CPCA23J1/14C11B1/025C11B1/04C11B3/006
Inventor 朱淑云梁嘉欣李欣贾瑞鹏张玉梅杨靖
Owner JIANGSU UNIV
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