Clonal propagation method of paphiopedilum armeniacum

A technology of asexual reproduction of Papudendron apricot, which is applied in the field of plant tissue culture, can solve the problems of slow growth of tissue culture seedlings and inconsistent products, and achieve the effects of short seedling growth period, high product consistency, and high induction rate of stems

Active Publication Date: 2019-01-25
GUANGXI FORESTRY RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problems of poor commerciality of the sexual reproduction products of P. apricotii and low success rate of asexual reproduction at present, the present invention provides a method for asexual reproduction of P. apricotii, that is, a method for rapid propagation of aseptic seedlings of P. apricotii by moving stems to establish The asexual reproduction technology system of P. chinensis solves the problems of slow growth and inconsistent products of tissue culture seedlings in production, improves the reproductive efficiency and productivity of P. chrysogenum seedlings, and meets the needs of large-scale planting of fine varieties of P. chrysogenum

Method used

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  • Clonal propagation method of paphiopedilum armeniacum

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Embodiment 1

[0030] A method for asexual propagation of Paphiopedilum apricot, comprising the steps of:

[0031] (1) Acquisition and processing of explants

[0032] In October, select the Vigorously growing Paphiopedilum apricot plant as the mother plant, move it into the greenhouse for cultivation, and induce the growth of stems and tillers. Before transplanting, use 800 times of potassium permanganate solution to sterilize the whole plant of P. apricotii by soaking for 3 minutes. The cultivation medium is peat: bark: sawdust: charcoal: sheep manure (V / V) = 3:3:2: 1:1 mixed evenly, the bark and sawdust are fully decomposed, and sterilized under high pressure at 121°C for 20 minutes before use; the cultivation container adopts a clay pot with a diameter of 12.5cm and a height of 10cm, and put it in a 500-fold potassium permanganate solution before use Soak for 30min to disinfect the container. Keep the temperature at 28°C, humidity at 60%, shade at 50%, and pour sterile water every 7 day...

Embodiment 2

[0049] A method for asexual propagation of Paphiopedilum apricot, comprising the steps of:

[0050] (1) Acquisition and processing of explants

[0051] In October, select a vigorously growing Paphiopedilum apricot plant as the mother plant, and move it into the greenhouse to induce stem-moving and tillering. Before transplanting, use 800 times of potassium permanganate solution to sterilize the whole plant of P. apricotii by soaking for 3 minutes. The cultivation medium is peat: bark: sawdust: charcoal: sheep manure (V / V) = 3:3:2: 1:1 mixed evenly, bark, sawdust, and sheep manure are fully decomposed, and sterilized by high pressure at 121°C for 20 minutes before use; the cultivation container is a clay pot with a diameter of 12.5cm and a height of 13cm, and the permanganate is 500 times higher before use. Soak in potassium solution for 30 minutes to disinfect the container. When planting, the roots are deeply buried, and the base of the stem is slightly exposed. Maintain a...

Embodiment 3

[0068] A method for asexual propagation of Paphiopedilum apricot, comprising the steps of:

[0069] (1) Acquisition and processing of explants

[0070] In October, select the Vigorously growing Paphiopedilum apricot plant as the mother plant, move it into the greenhouse for cultivation, and induce the growth of stems and tillers. Before transplanting, use 800 times of potassium permanganate solution to sterilize the whole plant of P. apricotii by soaking for 3 minutes. The cultivation medium is peat: bark: sawdust: charcoal: sheep manure (V / V) = 3:3:2: 1:1 mixed evenly, the bark and sawdust are fully decomposed, and sterilized under high pressure at 121°C for 20 minutes before use; the cultivation container adopts a clay pot with a diameter of 12.5cm and a height of 10cm, and put it in a 500-fold potassium permanganate solution before use Soak for 30min to disinfect the container. Keep the temperature at 28°C, humidity at 63%, shade at 55%, and pour sterile water every 8 day...

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a clonal propagation method of paphiopedilum armeniacum. The clonal propagation method of the paphiopedilum armeniacum comprises the following steps: (1) obtaining and treating of explants; (2) collection of the explants; (3) surface disinfection of the explants; (4) induction culture; (5) cluster budinduction; (6) multiplication culture; (7) seedling strengthening and rooting culture; (8) test-tube plantlet transplanting. Compared with the prior art, the clonal propagation method disclosed by the invention has the advantages that the explants are simple in material taking, are easy to obtain, are large in amount and cannot damage a stock plant, a tissue culture process is simple and feasible, the seedling formation period is short, the product consistency is high, the whole quality standard is controllable, and the like; the clonal propagation method is a simple, fast and effective propagation method for realizing commercialized production of the paphiopedilum armeniacum.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for asexual reproduction of Papudendron apricot. Background technique [0002] Paphiopedilum belongs to the orchid family Cypripedioideae. It is famous for its unique flower shape, rich and gorgeous flower colors, and long flowering period. It has high ornamental value and economic value. Apricot-yellow Paphiopedilum is a rare species of pure yellow flowers in the genus Paphiopedilum. It is one of the most ornamental species of the genus. It has won many international gold awards. ". [0003] However, due to the huge market value and the destruction of natural habitats, the wild resources of P. apricotii have been on the verge of extinction in recent years, and the market demand is increasing day by day. Therefore, the artificial rapid breeding and industrial cultivation of P. apricot is imminent. [0004] At present, the large-scale production of Paphiope...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈宝玲龚建英唐庆王华新林茂陈尔唐遒冥孙利娜苏莉花龙定建
Owner GUANGXI FORESTRY RES INST
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