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A method for detecting the density distribution of microplastics in soft tissues of marine organisms

A technology of marine organisms and density distribution, applied in the preparation of test samples, measuring devices, and analysis materials, etc., can solve the problems of high requirements for experimental conditions, low pretreatment and digestion rates, and damage to polymer structures. Reduce the effect of low risk factor, high digestion efficiency and improved accuracy

Active Publication Date: 2019-12-24
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid-base digestion method generally uses concentrated acid or alkali, which has a high risk, and has different effects on the properties of microplastics; some enzyme digestions are expensive, and are not suitable for large-scale investigations
At the same time, using a single stereomicroscope or fluorescence microscope to identify the components of microplastics usually leads to misjudgment; pyrolysis-gas phase-mass spectrometry will damage the structure of polymers and require high experimental conditions.
Although non-destructive techniques such as micro-Fourier spectroscopy, micro-Raman spectroscopy, and scanning electron microscopy-energy dispersive X-ray technology have also been applied to the identification of microplastics, due to the low sample pretreatment and digestion rate impact, resulting in lower detection accuracy

Method used

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  • A method for detecting the density distribution of microplastics in soft tissues of marine organisms
  • A method for detecting the density distribution of microplastics in soft tissues of marine organisms
  • A method for detecting the density distribution of microplastics in soft tissues of marine organisms

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Embodiment 1

[0060] (1) Select fish with similar biological individuals (see Table 1 for species), wash the digestive tract with pure water, drain the water, weigh and record the weight of the biological digestive tract;

[0061] (2) Put the drained digestive tract in a beaker, and add 30 mL of Fe with a concentration of 0.05 mol / L per 1 g of the digestive tract according to the weight of the biological digestive tract. 2+ solution, then add 30 mL of hydrogen peroxide solution with a mass fraction of 30%, and add in portions;

[0062] (3) Place the beaker on a magnetic stirrer for digestion at 60°C for 30 minutes until the digestion is complete to obtain a digestion mixture;

[0063] (4) Add 31.1g sodium chloride solid in every 100mL mixed liquor, carry out density flotation;

[0064] (5) Vacuum filter the flotation heavy liquid, pass through the membrane, and dry at room temperature for 24 hours. Through the microscope inspection of the filter, the suspected particles are picked for micr...

Embodiment 2

[0084] (1) Select bivalves with similar biological individuals (see Table 3 for species), wash the soft tissue with pure water, drain the water, weigh and record the weight of the biological soft tissue;

[0085] (2) Put the drained soft tissue in a beaker, add 30 mL of 0.1M Fe per 1 g of soft tissue according to the weight of the biological soft tissue 2+ solution, then add 30mL of hydrogen peroxide solution with a mass fraction of 32%, and add in portions;

[0086] (3) Place the beaker on a magnetic stirrer for digestion at 60°C for 30 minutes until the digestion is complete to obtain a digestion mixture;

[0087] (4) Add 31.1g sodium chloride solid in every 100mL mixed liquor, carry out density flotation;

[0088] (5) Vacuum filter the flotation heavy liquid, pass through the membrane, and dry at room temperature for 24 hours. Through the microscope inspection of the filter, the suspected particles are picked for microscopic-Fourier transform infrared spectroscopy and othe...

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Abstract

The invention discloses a method for detecting the density distribution of microplastics in soft tissues of marine organisms, and belongs to the field of microplastics in fish or bivalve biological samples. A method for detecting the density distribution of microplastics in soft tissues of marine organisms. The steps include: selecting fish or bivalve organisms that are close to each other, washing them with pure water to prepare digestive tract or soft tissue samples, and recording the weight; preparing a digestion solution to The prepared sample is digested and the digestion mixture is obtained; NaCl is added to the mixture for density flotation; the flotation particles are microscopically inspected, and suspected particles are selected for micro-Fourier spectroscopy analysis to identify microplastics. After counting, the density distribution of microplastics is calculated. The invention can accurately quantify microplastics in the digestive tract or soft tissue of marine organisms and provide basic data on the pollution status of microplastics in the area.

Description

technical field [0001] The invention belongs to the field of microplastics in fish or bivalve biological samples, and in particular relates to a method for detecting the density distribution of microplastics in soft tissues of marine organisms. Background technique [0002] The emergence of plastics has had a profound impact on people's lifestyles, and the output of plastics has also increased year by year. Currently, an estimated 2.5 billion tons of plastic are in use in the global market; meanwhile, from 1950 to 2015, 6.3 billion tons of primary plastics were produced and the resulting plastic waste. Plastic waste is difficult to degrade naturally in the environment, and will gradually decompose into particles with a particle size of less than 5mm through physical, chemical and biological effects. Generally, plastic particles with a particle size of less than 5 mm are defined as microplastics (Microplastics). Microplastics have the characteristics of small particle size,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10G01N1/28G01N1/34
CPCG01N1/28G01N1/34G01N15/10G01N2015/1022
Inventor 章春芳崔耀宗张冬冬周航海李艳红张立浩朱义年解庆林
Owner ZHEJIANG UNIV
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