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Oriented full-thickness cartilage scaffold containing calcified layer and preparation method thereof

A calcified layer and orientation technology, applied in the fields of biomaterials and biomedical engineering, can solve problems such as cartilage and bone microenvironment disorders, and achieve the effect of promoting differentiation

Active Publication Date: 2021-05-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem of disordered cartilage and bone microenvironment existing in the existing full-thickness cartilage scaffold materials, and prepares a full-thickness cartilage scaffold containing a calcified layer to stabilize the cartilage and bone microenvironment and promote the regeneration of cartilage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Prepare 0.3 mL of a mixed solution of silk fibroin and nano-hydroxyapatite hexafluoroisopropanol with a concentration of 12% and a mass ratio of 1:1, pour it into a mold, and dry it at room temperature to obtain a calcified bottom bracket;

[0014] 2) Prepare a compound solution with a total concentration of 4% according to the mass ratio of decellularized cartilage extracellular matrix and silk fibroin protein at 1:1. After mixing well, take 1 mL of the compound solution and add it to the surface of the calcified layer scaffold, and quickly carry out at -20°C. Gradient cooling to prepare oriented decellularized cartilage extracellular matrix and silk fibroin oriented upper layer scaffold. Finally, the whole scaffold was freeze-dried, treated with ethanol, and then freeze-dried for 48 hours to obtain the oriented full-thickness cartilage scaffold containing the calcified layer.

Embodiment 2

[0016] 1) Prepare 0.4 mL of a mixed solution of silk fibroin and β-tricalcium phosphate hexafluoroisopropanol with a concentration of 15% and a mass ratio of 1:1, pour it into a mold, and dry it at room temperature to obtain a calcified underlying scaffold;

[0017] 2) Prepare a compound solution with a total concentration of 5% according to the mass ratio of decellularized cartilage extracellular matrix and silk fibroin protein at 1:1. After mixing thoroughly, take 1 mL of the compound solution and add it to the surface of the calcified layer scaffold, and quickly carry out at -20 ° C. Gradient cooling to prepare oriented decellularized cartilage extracellular matrix and silk fibroin oriented upper layer scaffold. Finally, the whole scaffold was freeze-dried, treated with ethanol, and then freeze-dried for 48 hours to obtain the oriented full-thickness cartilage scaffold containing the calcified layer.

Embodiment 3

[0019] 1) 0.5 mL of a mixed solution of silk fibroin and bioactive glass hexafluoroisopropanol with a concentration of 20% and a mass ratio of 1:1 was prepared, and poured into a mold, and dried at room temperature to obtain a calcified underlying scaffold;

[0020] 2) Prepare a compound solution with a total concentration of 6% according to the mass ratio of the decellularized cartilage extracellular matrix and silk fibroin protein at a ratio of 1:1. After mixing thoroughly, take 1 mL of the compound solution and add it to the surface of the calcified layer scaffold, and quickly carry out at -20°C. Gradient cooling to prepare oriented decellularized cartilage extracellular matrix and silk fibroin oriented upper layer scaffold. Finally, the whole scaffold was freeze-dried, treated with ethanol, and then freeze-dried for 48 hours to obtain the oriented full-thickness cartilage scaffold containing the calcified layer.

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Abstract

The invention discloses an oriented full-thickness cartilage scaffold containing a calcification layer, which is similar to the structure of natural cartilage and consists of a double-layer structure of an oriented upper layer and a calcified bottom layer, wherein the oriented upper layer is composed of silk fibroin and decellularized cartilage extracellular matrix; calcification The bottom layer is composed of silk fibroin and any one of the three calcium and phosphorus inorganic salts of nano-hydroxyapatite, β-tricalcium phosphate, and bioactive glass. Through structural bionic design, the stability of cartilage and bone microenvironment can be achieved, and the purpose of effectively repairing cartilage defects can be achieved.

Description

technical field [0001] The invention relates to an oriented full-thickness cartilage scaffold containing a calcified layer and a preparation method thereof, in particular to making an oriented full-thickness cartilage scaffold containing a calcified layer by using silk fibroin composite material and heat-induced phase separation technology and a preparation method thereof. It belongs to the field of biomaterials and biomedical engineering. Background technique [0002] Combined cartilage damage caused by trauma, tumor resection, osteoarthritis, etc. is a relatively common disease in orthopedics. The current treatment methods for this kind of combined cartilage injury include autologous or allogeneic cartilage transplantation, but there are problems of donor site injury or immune rejection, and the overall treatment effect is not good, and breakthrough progress has not been made until now. Tissue engineering technology provides a new idea for the treatment of cartilage defec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/44A61L27/46A61L27/36A61L27/56A61L27/58A61F2/28
CPCA61F2/28A61L27/3633A61L27/44A61L27/46A61L27/56A61L27/58A61L2300/112A61L2300/412A61L2430/02A61L2430/06C08L89/00
Inventor 王淑芳董云生杨强张祥云
Owner NANKAI UNIV
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