Compound algicide and preparation method thereof
A technology of algaecide and additive, which is applied in the field of compound algaecide and its preparation, which can solve the problems of short maintenance time of algaecide and poor algae removal efficiency, and achieve the effect of increasing efficiency, improving separation ability, and maintaining ecological balance
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[0029] The preparation method of photosynthetically active additive is:
[0030] S1. Take Rhodopseudomonas palustris and inoculate it into the activation medium at an inoculum size of 7-10%, incubate at a light intensity of 3000lx and 25-28°C for 4-7 days, centrifuge, take the precipitate a, take the precipitate a and press Mass ratio 1~4:10~20 Add Tris-HCl buffer solution with pH 8.0 and concentration of 10mmol / L, shake for 20~30min, centrifuge, take the precipitate b, take the precipitate b according to the mass ratio 1~3:5~8 Add the dissolving solution and mix, keep warm at -30~-20°C for 30~45min, thaw, repeat the warming and thawing process for 2~4 times, and sonicate for 10~15min to obtain the ultrasonic solution;
[0031] S2. Take the ultrasonic liquid and add polyethylene glycol octyl phenyl ether and sodium chloride according to the mass ratio of 100~200:1~3:0.1~0.3, stir and mix at 25~30°C for 1~3h, and then in 1~4 Stand still at ℃ for 12~15h, centrifuge, take the su...
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