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Sericin/oxidized graphene composite hydrogel as well as preparation method and application thereof

A graphene composite and hydrogel technology, applied in the field of biomedical composite materials, can solve problems such as limited donor sources, achieve good biodegradability, promote adhesion and proliferation, and have simple and feasible preparation methods

Inactive Publication Date: 2018-12-21
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although autologous transplantation has a good osteoinductive effect, the source of donors is limited and a second operation is required

Method used

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  • Sericin/oxidized graphene composite hydrogel as well as preparation method and application thereof
  • Sericin/oxidized graphene composite hydrogel as well as preparation method and application thereof
  • Sericin/oxidized graphene composite hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of sericin / graphene oxide composite hydrogel provided by the present invention

[0056] Step 1) Extraction of Sericin

[0057] 1) Weigh 10 g silk fibroin-deficient mutant cocoon 140 N-ds , cut into 1 cm 2 The fragments were placed in a clean beaker, washed 3 times with deionized water, and centrifuged to remove excess water;

[0058] 2) Add the cocoon fragments obtained in step 1) to 200-400 mL of Na with a molar concentration of 0.02 mol / L 2 CO 3 In the solution, stir and react in a water bath at 100°C for 1 hour to completely dissolve the sericin and obtain a sericin solution;

[0059] 3) Place the sericin solution obtained in step 2) in a 50 mL centrifuge tube and centrifuge at 3500 rpm for 5 minutes to remove insoluble precipitates and obtain a clarified sericin solution;

[0060] 4) Add the sericin solution obtained in step 3) into a dialysis bag with a molecular weight cut-off of 3.5 kDa, place it in a beaker containing 1 L of deionized...

Embodiment 2

[0078] Example 2 Preparation of sericin / graphene oxide composite hydrogel provided by the present invention

[0079] The preparation method of the sericin / graphene oxide mixed solution in this example is the same as that of Example 1, the difference is that step 3) is carried out as follows:

[0080] 1) Dissolve 3 g of methacrylic anhydride-modified sericin powder in 20 mL of deionized water to prepare a 15% (W / V) methacrylic anhydride-modified sericin solution;

[0081] 2) Weigh 40 mg of graphene oxide and disperse it in 10 mL of deionized water to make a 4 mg / mL graphene oxide dispersion, and ultrasonically disperse it for 10-30 minutes to make it uniform;

[0082] 3) The ultrasonically dispersed graphene oxide dispersion liquid and the methacrylic acid-modified sericin solution were prepared into a mixed solution at a volume ratio of 1:17, specifically, a 200 μL system containing 4 mg / mL graphene oxide 10 μL and 170 μL of 15% sericin solution;

[0083] 4) Irgacure 2959 po...

Embodiment 3

[0086] Example 3 Preparation of sericin / graphene oxide composite hydrogel provided by the present invention

[0087] The preparation method of the sericin graphene oxide mixed solution in this example is the same as that in Example 1, except that step 3) is carried out as follows:

[0088] 1) Weigh 3 g of methacrylic anhydride-modified sericin powder and dissolve it in 20 mL of deionized water to prepare a 15 % (W / V) methacrylic anhydride-modified sericin solution;

[0089] 2) Weigh 40 mg of graphene oxide and dissolve it in 10 mL of deionized water to make a 4 mg / mL graphene oxide dispersion, and ultrasonically disperse it with water for 10-30 minutes;

[0090] 3) The ultrasonically dispersed graphene oxide dispersion and the methacrylic acid-modified sericin solution were prepared into a mixed solution at a ratio of 1:8 by volume, specifically, a 200 μL system containing 20 μL of 4 mg / mL graphene oxide and 160 μL of 15% sericin solution;

[0091] 4) Irgacure 2959 powder is...

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Abstract

The invention relates to sericin / oxidized graphene composite hdyrogel as well as a preparation method and application thereof. The preparation method comprises the steps: blending a methacrylic anhydride modified sericin solution and oxidized graphene, then adding a photoinitiator and performing crosslinking under ultraviolet irradiation to prepare sericin / oxidized graphene composite hydrogel withgood mechanical property and osteogenic induction performance. The prepared sericin / oxidized graphene composite hydrogel has the advantages of good biocompatibility and biodegradability, high mechanical strength and osteogenic induction and application to the aspects of medicine and cell carrying, tissue engineering repairing and the like.

Description

technical field [0001] The invention relates to the field of biomedical composite materials, and specifically refers to a preparation method and application of a sericin / graphene oxide composite hydrogel. The composite hydrogel has good biocompatibility and biodegradability, and can promote bone Tissue growth has a good application prospect in tissue damage repair. Background technique [0002] Large-area bone defects are very common in clinical practice, mainly caused by trauma, infection, tumor, pathological fracture, etc. Traditional bone defect treatment methods include autograft, allograft, and artificial bone implantation. Although autologous transplantation has a good osteoinductive effect, the source of donors is limited and a second operation is required. Allogeneic transplantation has immune rejection that needs to be overcome. Artificial bones such as bioceramics and titanium alloys also face problems such as osteogenesis difficulties. With the rapid develop...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/28C08L89/00C08K3/04A61L27/44A61L27/50A61L27/52A61L27/58
CPCA61L27/446A61L27/50A61L27/52A61L27/58A61L2400/06A61L2430/02A61L2430/34C08J3/075C08J3/28C08J2389/00C08K3/042C08L89/00
Inventor 王琳王征乞超邓炎刘佳万超朱媛媛
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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