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Oral ulcer ointment prepared by umbilical cord mesenchymal stem cell to secrete cytokine and preparation method thereof

A technology of stem cells and cytokines, applied in the field of stem cells, can solve problems such as unacceptable, oral mucosal irritation, adverse reactions, etc., and achieve the effects of preventing contact friction, improving the immune system, and reducing pain

Inactive Publication Date: 2018-12-18
北京壹典壹生生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because recurrent oral ulcers are autoimmune diseases caused by autoimmune system disorders, the above drug treatments have certain limitations, are difficult to treat fundamentally, and will also cause certain adverse reactions, such as oral mucosa The irritation of the patient, the patient feels uncomfortable, difficult to accept, etc., and some drugs will also produce systemic side effects

Method used

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  • Oral ulcer ointment prepared by umbilical cord mesenchymal stem cell to secrete cytokine and preparation method thereof
  • Oral ulcer ointment prepared by umbilical cord mesenchymal stem cell to secrete cytokine and preparation method thereof

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Embodiment 1

[0027] Obtaining of umbilical cord mesenchymal stem cells: Aseptically take the umbilical cord and cut it into small pieces of about 3 cm, discard the blood vessels, take the perivascular material and cut them into pieces, first use 1‰ type IV collagenase to digest for 2 hours, and then centrifuge at 2000r / min for 10 minutes, Take the lower layer of pellet and continue to digest with 0.25% trypsin-PBS at 37°C for 25min, then centrifuge at 2000r / min for 10min, take the cell pellet and digest it with 0.25% trypsin solution, then centrifuge at 800r / min for 10min, take the cell pellet and wash it with 1× 10 6 The cells / ml density is suspended in the culture medium, and the culture medium is a commercially available serum-free medium for mesenchymal stem cells. Set at 37°C, 100% humidity, 5% CO 2 Cultivate in a dark environment, change the medium every 3 days, after 2 days, fibroblasts can be seen growing at the bottom of the bottle, and after about 10 days, they can reach 80% con...

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Abstract

The invention discloses an oral ulcer ointment prepared by umbilical cord mesenchymal stem cell to secrete cytokine and a preparation method thereof. The ointment can significantly improve the recovery rate of patients with oral ulcers and effectively prevent the recurrence rate. The method comprises the following specific steps: stimulating umbilical cord mesenchymal stem cells with calcium chloride and gamma-interferon to secrete more immunoregulatory cytokines, collecting a culture supernatant to prepare freeze-drying powder, mixing honeysuckle flower, isatis root, dandelion, folium isatidis, polyethylene glycol, maltodextrin and xanthan gum, and stirring a mixture under low temperature conditions to form an oral ulcer ointment. The oral ulcer ointment of the present invention is applied to the local part of patients with the oral ulcer, the treatment time and the recurrence rate are statistically determined, and the result indicates that the oral ulcer ointment can effectively promote the healing time of patients with the oral ulcer and effectively prevents recurrence.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to an oral ulcer ointment prepared by secreting cytokines from umbilical cord mesenchymal stem cells and a preparation method thereof. Background technique [0002] Recurrent oral ulcer (ROU) is an oral mucosal disease that often occurs on the lips, tongue, cheeks, and soft palate. It is often accompanied by spontaneous pain, which is easy to recur and seriously affects the quality of life of patients. The pathogenesis of recurrent oral ulcer mainly includes factors such as autoimmune disorder, local injury, mental stress, and diet. At present, there is no effective method for radically curing recurrent oral ulcers, which can only be treated by reducing the number of recurrences, prolonging the interval between attacks, relieving pain and promoting healing. The cause of disease of oral ulcer mainly contains following several kinds: 1) autoimmune reaction, finds through research ...

Claims

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Application Information

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IPC IPC(8): A61K36/855A61K36/704A61K36/355A61K9/06A61P1/02A61K35/28
CPCA61K9/06A61K35/28A61K36/195A61K36/288A61K36/315A61K36/355A61K36/704A61K36/855A61K47/10A61K47/36A61P1/02A61K2300/00
Inventor 李栋马步鹏宋芸娟
Owner 北京壹典壹生生物技术有限公司
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