Application of cowherb seed flavonoid glycoside capable of preventing microcirculation disturbance

A kind of Wangbuliuxing flavonoid glycosides, microcirculation disorder technology, applied in the field of drug development

Inactive Publication Date: 2018-12-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Now, some studies have proved that the flavonoid glycosides of wangbuliuxing have the functions of invigorating blood circulation, promoting menstrual flow, reducing breast swelling, diuresis and treating stranguria. Relevant literature reports (from CNKI, Wanfang, VIP China Journal Network), therefore, the research fields of the pharmacology and drug efficacy of blancaliza glycosides in anti-microcirculatory disturbance are still in a blank stage

Method used

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  • Application of cowherb seed flavonoid glycoside capable of preventing microcirculation disturbance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of varicose flavonoid glycosides

[0024] Specific steps are as follows:

[0025] (1) Grind blanc blanc into pieces, degrease with petroleum ether and weigh 50g;

[0026] (2) 50 g of the obtained defatted bulipel was extracted with 75% ethanol under reflux for 90 min, and the filtrate was extracted twice and combined twice;

[0027] (3) Redissolve the obtained filtrate with deionized water after spin-drying, then extract with water-saturated n-butanol to obtain a water-saturated n-butanol extraction part;

[0028] (4) Spin-dry the obtained water-saturated n-butanol extraction part and reconstitute it with ionized water to form a 2 mg / mL medicinal solution;

[0029] (5) Soak the HPD-100 macroporous resin in 95% ethanol overnight, stir it evenly with a glass rod and drain it into a glass column with a diameter of 2.5cm and control the ratio of diameter to height to 1:7 (18cm, 2.5cm) ;

[0030] (6) Wash the macroporous resin with 95% ethan...

Embodiment 2

[0033] Embodiment 2: the preparation of mouse model

[0034] Fifty C57BL / 6 mice were randomly divided into 5 groups, namely, blank control group, model group, high-dose agaricus flavonoid glycosides group, low-dose acaricus glucosides group and positive drug group, 10 in each group. Only, wherein, the blank control group was fed with common feed, and the remaining four groups were fed with high-fat feed (the high-fat feed contained 1% lard, 5% egg yolk powder, 1% cholesterol, 10% sugar, 0.2% sodium cholate) for a total of 8 weeks.

Embodiment 3

[0035] Embodiment 3: Administration of mice

[0036] After 8 weeks, the blank control group obtained in Example 2 was injected intraperitoneally with 0.4 mL of normal saline every day, and the flavonoid glucoside high and low dose groups were fed with 0.4 mL of 1 mg / kg and 10 mg / kg respectively. 0.05mg / mL, 0.5mg / mL of the flavonoid glycoside solution of blalicius (formed from the flavonoid glycoside obtained in Example 1 and normal saline), and the positive drug group was intraperitoneally injected with a concentration of 0.4mL at 200mg / kg. 0.025 mg / mL metformin solution (prepared from metformin purchased from Sigma and normal saline).

[0037] The basic conditions of the mice, such as body weight and living conditions, were recorded for a total of 12 weeks of administration.

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Abstract

The invention discloses an application of cowherb seed flavonoid glycoside capable of preventing the microcirculation disturbance, and belongs to the technical field of drug development. For the firsttime, people find that cowherb seed flavonoid glycoside has a function of preventing the microcirculation disturbance, and can be used to prevent and / or treat diseases caused by microcirculation disturbance such as liver cancer, lung cancer, pancreatic cancer, hypertension, coronary heart disease, periarthritis of shoulder, irregular menstruation, appendagitis, myoma of uterus, arrhythmia, angina, and the like. Cowherb seed flavonoid glycoside can be used to prepare medicinal preparations, food, nutriments, health care products, and cosmetics.

Description

technical field [0001] The invention relates to the application of buliurin flavonoid glycoside for resisting microcirculation disturbance, and belongs to the technical field of drug development. Background technique [0002] Microcirculation is the blood circulation in capillaries between arterioles and venules. It is the most basic structural and functional unit in the circulatory system. It includes body fluid circulation in arterioles, venules, capillaries and tissue channels. Every organ and every tissue cell in the human body must be supplied with oxygen and nutrients by the microcirculation, transmit energy, exchange information, and remove carbon dioxide and metabolic waste. [0003] Microcirculatory disturbance is a series of clinical symptoms caused by lumen stenosis, blood flow slowdown, thrombosis, local tissue ischemia and hypoxia or even necrosis caused by changes in blood physicochemical properties. At the same time, medicine has proved that: microcirculation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/14A61P35/00A61P39/06A61P15/00A61P9/00
CPCA61K31/7048A61P9/00A61P9/14A61P15/00A61P35/00A61P39/06
Inventor 邱丽颖龚蕾蕾蔡维维沈后望童玲洁朱敏勇梅颖张宏丹
Owner JIANGNAN UNIV
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