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Cell culture plate for analyzing biological function of bone

A cell culture and functional analysis technology, applied in animal cells, cell culture supports/coatings, vertebrate cells, etc., can solve the problems of limiting cell differentiation and functional research, expensive products, etc., to improve osteoinductive effect and The ability to carry cells, excellent biocompatibility, and the effect of thin coating thickness

Inactive Publication Date: 2018-12-07
赵亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no commercial product available in China, and we can only rely on imports from Sigma Corporation of the United States. The related products are extremely expensive, which largely limits the clinical application of cell differentiation and function research related to osteoblast biology

Method used

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  • Cell culture plate for analyzing biological function of bone
  • Cell culture plate for analyzing biological function of bone
  • Cell culture plate for analyzing biological function of bone

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 are calcined at 1500°C to form tetracalcium phosphate, which is ground, stirred and sieved to obtain tetracalcium phosphate particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain calcium hydrogen phosphate particles with a diameter of 0.4-3.0 μm. Tetracalcium phosphate (MW=366.254g / mol) / calcium hydrogen phosphate (MW=136.057g / mol) is mixed in a molar mass ratio of 1 / 3 to prepare calcium phosphate composite powder, and 1%-5% of mass percentage of citric acid tri Sodium, building bone substitute material. ddH 2 O (2.5% W / V) double distilled water was used as the liquid phase. Add 100mLddH to 250mg bone substitute material 2 O (2.5% W / V) double distilled water to obtain 2.5mg / mL bone substitute material stock solution, ultrasonically stirred (50% vibration amplitude, 20 seconds) to form a uniform bone substitute material suspension, measure the microsp...

Embodiment 2

[0031] Anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 are calcined at a high temperature of 1400°C to form tetracalcium phosphate, which is ground, stirred and sieved to obtain tetracalcium phosphate particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain calcium hydrogen phosphate particles with a diameter of 0.4-3.0 μm. Tetracalcium phosphate (MW=366.254g / mol) / calcium hydrogen phosphate (MW=136.057g / mol) is mixed at a molar mass ratio of 1 / 2.5 to prepare calcium phosphate composite powder, and 1%-5% of mass percentage of citric acid tri Sodium, building bone substitute material. ddH 2 O (2.5% W / V) double distilled water was used as the liquid phase. Add 100mLddH to 250mg bone substitute material 2 O (2.5% W / V) double distilled water to obtain 2.5mg / mL bone substitute material stock solution, ultrasonically stirred (50% vibration amplitude, 20 seconds) to form a uniform bone substitute material suspens...

Embodiment 3

[0033] Anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 are calcined at 1500°C to form tetracalcium phosphate, which is ground, stirred and sieved to obtain tetracalcium phosphate particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain calcium hydrogen phosphate particles with a diameter of 0.4-3.0 μm. Tetracalcium phosphate (MW=366.254g / mol) / calcium hydrogen phosphate (MW=136.057g / mol) is mixed at a molar mass ratio of 1 / 2 to prepare calcium phosphate composite powder, and 1%-5% of citric acid tri Sodium, building bone substitute material. ddH 2 O (2.5% W / V) double distilled water was used as the liquid phase. Add 100mLddH to 250mg bone substitute material 2O (2.5% W / V) double distilled water to obtain 2.5mg / mL bone substitute material stock solution, ultrasonically stirred (50% vibration amplitude, 20 seconds) to form a uniform bone substitute material suspension, measure the microsphere diameter and in...

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Abstract

The invention discloses a cell culture plate for analyzing the biological function of bone. The culture culture plate can realize direct microscopic observation of the cell function activity, and is mainly used for evaluating the cell function activity to drug treatment response, especially biologically-related cell differentiation and functional study on phagocytosis of osteoclasts and osteogenesis of osteoblasts and the like. The technical scheme is finished by the following steps: adopting the cell culture plate with at least one hole, adding 100-500mum of bone substitution material suspension based on calcium phosphate and with the concentration of 1mg-10mg / ml to the bottom part of the culture plate, carrying out water bathing for 6-24 hours at the temperature of 37 DEG C, vibrating for 15-30 seconds every half an hour at a vibration frequency of 60 Hz, using a shaking table to shake for 5-10 minutes uniformly each hour at a frequency of 15-30 times per minute, after the suspensionis dried, drying for 4-10 hours in a drying oven at the temperature of 50 DEG C, at the moment, uniformly attaching the bone substitution material on the bottom part of the culture plate to form a film-plated coating layer with the thickness of 50-200mum and made of transparent material, carrying out aseptic vacuum package and using gamma rays for sterilization.

Description

technical field [0001] The invention discloses a bone biological function analysis cell culture plate, specifically provides a cell culture plate with an ideal bone biological function, which can directly observe the cell function activity under a microscope, and is mainly used for evaluating the cell function in response to drug treatment Activity, especially biologically relevant cell differentiation and function studies such as phagocytosis of osteoclasts and osteogenesis of osteoblasts, is an important tool for the study of biological and molecular mechanisms of bone cells. Background technique [0002] Bone biological function analysis cell culture plate is used to evaluate the functional activity of cells in response to drug treatment, mainly used for cell differentiation and function research related to osteoclast and osteoblast biology, and is an important tool for the study of bone cell biology and molecular mechanism . At present, there is no commercial product av...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCC12M23/12C12M23/20C12N5/0643C12N5/0654C12N2533/18G01N33/5044
Inventor 赵亮赵宝红包良笑
Owner 赵亮
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