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Bone biological functional analysis porous cell culture plate

A technology for cell culture and functional analysis, applied in the field of cell culture plates, which can solve the problems of expensive products, limiting cell differentiation and functional research, etc., achieve excellent biocompatibility, promote differentiation and functional research applications, and reduce surface degeneration Effect

Inactive Publication Date: 2017-12-26
赵亮 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no commercial product available in China, and we can only rely on imports from Sigma Corporation of the United States. The related products are extremely expensive, which largely limits the clinical application of cell differentiation and function research related to osteoblast biology

Method used

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  • Bone biological functional analysis porous cell culture plate
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Effect test

Embodiment 1

[0029] Calcining anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 at 1500°C to produce TTCP, grinding, stirring and sieving to obtain TTCP particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain DCPA particles with a diameter of 0.4-3.0 μm. TTCP (MW=366.254g / mol) / DCPA (MW=136.057g / mol) were mixed at a molar mass ratio of 1 / 3 to prepare CPC powder. ddH 2 O (2.5% W / V) double distilled water as the liquid phase. Add 100mLddH to 250mgCPC 2 O (2.5% W / V) double-distilled water to obtain 2.5 mg / mL CPC stock solution, ultrasonically stirred (50% vibration amplitude, 20 seconds) to form a uniform CPC suspension, and measured the microsphere diameter, internal structure, and morphology for future use. For a conventional 96-well cell culture plate, add 50ul of CPC suspension to each well and pour it to the bottom of the cell culture plate, bathe in water at 37°C until the suspension is dry, and sterilize it with gam...

Embodiment 2

[0031] Calcining anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 at 1400°C to produce TTCP, grinding, stirring and sieving to obtain TTCP particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain DCPA particles with a diameter of 0.4-3.0 μm. TTCP (MW=366.254g / mol) / DCPA (MW=136.057g / mol) was mixed at a molar mass ratio of 1 / 2.5 to prepare CPC powder. ddH 2 O (2.5% W / V) double distilled water as the liquid phase. Add 100mLddH to 250mgCPC 2 O (2.5% W / V) double-distilled water to obtain 2.5 mg / mL CPC stock solution, ultrasonically stirred (50% vibration amplitude, 20 seconds) to form a uniform CPC suspension, and measured the microsphere diameter, internal structure, and morphology for future use. For a conventional 96-well cell culture plate, add 100ul of CPC suspension to each well and pour it to the bottom of the cell culture plate, put it in a 37°C water bath until the suspension is dry, and sterilize it w...

Embodiment 3

[0033] Calcining anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 at 1500°C to produce TTCP, grinding, stirring and sieving to obtain TTCP particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain DCPA particles with a diameter of 0.4-3.0 μm. TTCP (MW=366.254g / mol) / DCPA (MW=136.057g / mol) were mixed at a molar mass ratio of 1 / 2 to prepare CPC powder. ddH 2 O (2.5% W / V) double distilled water as the liquid phase. Add 100mLddH to 250mgCPC 2 O (2.5% W / V) double-distilled water to obtain 2.5mg / mL CPC stock solution, ultrasonic stirring (50% vibration amplitude, 20 seconds) to form a uniform CPC suspension, and measure the microsphere diameter and internal structure and morphology for future use. For a conventional 96-well cell culture plate, add 150ul of CPC suspension to each well of the cell culture plate, place it in a 37°C water bath until the suspension is dry, and sterilize it with gamma rays before use. ...

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Abstract

The invention discloses a porous cell culture plate for bone biological function analysis, aiming to provide a cell culture plate with uniform calcium phosphate complex bridging, complete surface structure, and ideal bone biomimetic synthesis surface structure; its technical scheme is adopted Preparation by the following steps: high temperature calcining of anhydrous calcium hydrogen phosphate and calcium carbonate at 1400-1600°C to form TTCP, grinding, stirring and sieving to obtain TTCP particles with a diameter of 1-80 μm; grinding, stirring and sieving to obtain DCPA particles with a diameter of 0.4-3.0 μm; Mix TTCP and DCPA at a molar mass ratio of 1-3:3-1 to prepare CPC powder, and use double-distilled water as the liquid phase to prepare liquid-phase CPC; then add double-distilled water and ultrasonically stir to obtain a CPC stock solution with a solubility of 1mg-5mg / ml CPC suspension.

Description

technical field [0001] The invention discloses a cell culture plate, in particular, a porous cell culture plate for bone biological function analysis. Background technique [0002] Bone biological function analysis multi-well cell culture plate is used to evaluate the functional activity of cells in response to drug treatment, mainly used for cell differentiation and function research related to osteoblast biology, and is an important tool for the study of bone cell biology and molecular mechanism. At present, there is no commercial product available in China, and we can only rely on imports from Sigma Corporation of the United States. The related products are extremely expensive, which largely limits the clinical application of cell differentiation and function research related to osteoblast biology. Contents of the invention [0003] In view of the above-mentioned deficiencies, the purpose of the present invention is to provide a calcium phosphate compound based bone b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04C12N5/077C12N5/078
CPCC12M23/12C12M23/20C12N5/0643C12N5/0654C12N2533/18
Inventor 赵亮赵宝红包良笑
Owner 赵亮
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