Bone biological functional analysis porous cell culture plate
A technology for cell culture and functional analysis, applied in the field of cell culture plates, which can solve the problems of expensive products, limiting cell differentiation and functional research, etc., achieve excellent biocompatibility, promote differentiation and functional research applications, and reduce surface degeneration Effect
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Embodiment 1
[0029] Calcining anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 at 1500°C to produce TTCP, grinding, stirring and sieving to obtain TTCP particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain DCPA particles with a diameter of 0.4-3.0 μm. TTCP (MW=366.254g / mol) / DCPA (MW=136.057g / mol) were mixed at a molar mass ratio of 1 / 3 to prepare CPC powder. ddH 2 O (2.5% W / V) double distilled water as the liquid phase. Add 100mLddH to 250mgCPC 2 O (2.5% W / V) double-distilled water to obtain 2.5 mg / mL CPC stock solution, ultrasonically stirred (50% vibration amplitude, 20 seconds) to form a uniform CPC suspension, and measured the microsphere diameter, internal structure, and morphology for future use. For a conventional 96-well cell culture plate, add 50ul of CPC suspension to each well and pour it to the bottom of the cell culture plate, bathe in water at 37°C until the suspension is dry, and sterilize it with gam...
Embodiment 2
[0031] Calcining anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 at 1400°C to produce TTCP, grinding, stirring and sieving to obtain TTCP particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain DCPA particles with a diameter of 0.4-3.0 μm. TTCP (MW=366.254g / mol) / DCPA (MW=136.057g / mol) was mixed at a molar mass ratio of 1 / 2.5 to prepare CPC powder. ddH 2 O (2.5% W / V) double distilled water as the liquid phase. Add 100mLddH to 250mgCPC 2 O (2.5% W / V) double-distilled water to obtain 2.5 mg / mL CPC stock solution, ultrasonically stirred (50% vibration amplitude, 20 seconds) to form a uniform CPC suspension, and measured the microsphere diameter, internal structure, and morphology for future use. For a conventional 96-well cell culture plate, add 100ul of CPC suspension to each well and pour it to the bottom of the cell culture plate, put it in a 37°C water bath until the suspension is dry, and sterilize it w...
Embodiment 3
[0033] Calcining anhydrous calcium hydrogen phosphate and calcium carbonate with a weight ratio of 136.06:100.09 at 1500°C to produce TTCP, grinding, stirring and sieving to obtain TTCP particles with a diameter of 1-80 μm. Grinding, stirring and sieving to obtain DCPA particles with a diameter of 0.4-3.0 μm. TTCP (MW=366.254g / mol) / DCPA (MW=136.057g / mol) were mixed at a molar mass ratio of 1 / 2 to prepare CPC powder. ddH 2 O (2.5% W / V) double distilled water as the liquid phase. Add 100mLddH to 250mgCPC 2 O (2.5% W / V) double-distilled water to obtain 2.5mg / mL CPC stock solution, ultrasonic stirring (50% vibration amplitude, 20 seconds) to form a uniform CPC suspension, and measure the microsphere diameter and internal structure and morphology for future use. For a conventional 96-well cell culture plate, add 150ul of CPC suspension to each well of the cell culture plate, place it in a 37°C water bath until the suspension is dry, and sterilize it with gamma rays before use. ...
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