Alliin antibody prepared by immunization of guinea pigs

An alliin, guinea pig technology, applied in the direction of anti-plant immunoglobulin, serum immunoglobulin, etc., can solve the problem of lack of immune antigen and antibody research.

Active Publication Date: 2020-06-09
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention intends to provide an alliin antibody prepared by immunization of guinea pigs, so as to solve the problem that there is no research on alliin-related immune antigens and antibodies in the prior art

Method used

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  • Alliin antibody prepared by immunization of guinea pigs
  • Alliin antibody prepared by immunization of guinea pigs

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Experimental program
Comparison scheme
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Embodiment Construction

[0020] Further detailed explanation through specific implementation mode below:

[0021] Antigen preparation

[0022] Preparation of alliin antigen: Add 10ml of egg white to 2ml of alliin solution with a mass concentration of 100mg / ml and stir well to mix well. The milky white alliin and egg white complex precipitates to form alliin antigen.

[0023] The preparation of adjuvant antigen: take lanolin 5g, get paraffin oil 20ml, autoclave for standby, be Freund's incomplete adjuvant, get Freund's incomplete adjuvant 10ml, add 60mg BCG drop by drop under aseptic condition, add While grinding, it is Freund's complete adjuvant; then add the same amount of alliin antigen drop by drop, and also add while grinding to form a stable suspension to obtain the adjuvant antigen.

[0024] animal immunity

[0025] Guinea pigs require a body weight of 300-500g. Immunization injections are carried out according to Table 1. Pay attention to the requirements of cardiac injections. One dose is di...

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Abstract

The invention discloses an alliin antibody prepared through the immunization of guinea pigs. The alliin antibody contains an alliin antigen and a Freund's complete adjuvant, the alliin antigen and theFreund's complete adjuvant with equal amount are mixed to obtain an adjuvant antigen, the alliin antigen is injected into the body of a guinea pig, and the alliin antibody is obtained after the immune response. According to the alliin antibody, the immunogenicity of alliin is solved, the antibody is acquired through the immunization of the guinea pigs, and the alliin antibody can be applied to research of the concentration detection, in-vivo distribution and pharmacological action mechanisms of alliin and has important application values in the foundation and clinical researches of alliin.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to an alliin antibody prepared by immunizing guinea pigs. Background technique [0002] Alliin is a water-soluble amino acid compound of garlic, with stable chemical properties. In recent years, in vitro experiments have found that alliin has antimicrobial, antitumor, antioxidation, immune regulation and cardiovascular disease prevention effects, and no clinical research has been seen so far. reports. [0003] Low-molecular compounds are generally not immunogenic. When combined with high-molecular compounds, they can activate the body's immune cells and produce corresponding antibodies. Alliin is a low-molecular-weight amino acid compound and has no immunogenicity itself. However, alliin has amino, carboxyl and thioether groups, which can be combined with protein molecules. Whether the combination is immunogenic and stimulates the body to produce antibod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/16C07K16/06
CPCC07K16/06C07K16/16
Inventor 李明强韩志俊宋姗姗裴天容
Owner ZUNYI MEDICAL UNIVERSITY
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