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Degradable artificial dura mater with double-layer structure and preparation method of degradable artificial dura mater

A technology of artificial dura mater and double-layer structure, applied in prosthetics, medical science, etc., can solve problems such as lack, and achieve the effects of controllable production process, single component, and simple process method

Active Publication Date: 2022-03-25
西安臻研生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with other similar materials with collagen as the main component, artificial synthetic materials lack the biological functions mediated by collagen, such as promoting cell migration and proliferation, and promoting the secretion of related cytokines.

Method used

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  • Degradable artificial dura mater with double-layer structure and preparation method of degradable artificial dura mater
  • Degradable artificial dura mater with double-layer structure and preparation method of degradable artificial dura mater
  • Degradable artificial dura mater with double-layer structure and preparation method of degradable artificial dura mater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Preparation of the scaffold layer: prepare a recombinant human-like collagen type I solution with a mass concentration of 30% in purified water, and after it dissolves, slowly add a cross-linking agent BDDE with a mass concentration of 0.2% in a stirring state to make the cross-linked The good gel was left to stand for 1 hour, and then put into a dialysis bag; put the dialysis bag in a buffer solution of pH 7.4, add a stirring device to continue stirring, change the liquid every 2 hours, and stop when there is no free BDDE in the dialysate Stir. Pour out the solution in the dialysis bag and stir evenly to obtain cross-linked collagen.

[0044] Then use purified water to prepare a recombinant type I humanoid collagen solution with a mass concentration of 20%. The recombinant type I human-like collagen solution, the mass ratio of the recombinant type I human-like collagen in the two is 3:2 for mixing), then spray horizontally or coat on a flat plate with a thickness ...

Embodiment 2

[0048] (1) Preparation of the scaffold layer: prepare a recombinant human-like collagen type I solution with a mass concentration of 20% in purified water, and after it dissolves, slowly add a cross-linking agent BDDE with a mass concentration of 0.1% under stirring to dissolve the cross-linked collagen The good gel was left to stand for 1 hour, and then put into a dialysis bag; put the dialysis bag in a buffer solution of pH 7.4, add a stirring device to continue stirring, change the liquid every 2 hours, and stop when there is no free BDDE in the dialysate Stir. Pour out the solution in the dialysis bag and stir evenly to obtain cross-linked collagen.

[0049] Then use purified water to prepare a recombinant human-like collagen type I solution with a mass concentration of 30%. After it dissolves, mix it with cross-linked collagen, and then spray it horizontally or spread it on a flat plate with a thickness of 1.0-1.5mm, and let it dry at room temperature for 2 After ~4h, wh...

Embodiment 3

[0056] (1) Preparation of the scaffold layer: prepare a recombinant human-like collagen type I solution with a mass concentration of 40% in purified water. The good gel was left to stand for 1 hour, and then put into a dialysis bag; put the dialysis bag in a buffer solution of pH 7.4, add a stirring device to continue stirring, change the liquid every 2 hours, and stop when there is no free BDDE in the dialysate Stir. Pour out the solution in the dialysis bag and stir evenly to obtain cross-linked collagen.

[0057] Then use purified water to prepare a recombinant human-like collagen type I solution with a mass concentration of 20%. After it dissolves, mix it with cross-linked collagen, and then spray it horizontally or spread it on a flat plate with a thickness of 1-1.5mm, and let it dry at room temperature for 2 ~4h, when the thickness decreases by 0.3-0.6mm, the scaffold layer is obtained; the scaffold layer is placed in a -20°C refrigerator to freeze, and the scaffold lay...

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Abstract

The invention relates to a degradable artificial dura mater with a double-layer structure and a preparation method of the degradable artificial dura mater, and relates to the technical field of biomedical materials. The degradable artificial dura mater with the double-layer structure comprises a stent layer and a bonding layer, the thickness of the artificial dura mater is 0.5-1.0 mm, the stent layer is made of recombinant human-like collagen, and the bonding layer is made of mussel mucin. According to the degradable artificial dura mater with the double-layer structure, the recombinant human-like collagen and the mussel mucin serve as main raw materials, and the artificial dura mater is a non-animal-derived material, so that the immunogenicity problem can be avoided; the artificial dura mater disclosed by the invention has a double-layer structure, degradability, good flexibility, adhesion, mechanical property and biocompatibility; meanwhile, according to the preparation method of the artificial dura mater, recombinant human-like collagen and mussel mucin serve as main raw materials, the degradable artificial dura mater of the double-layer structure is prepared through the processes of dissolution, crosslinking, dialysis, mixing, coating, drying and the like, the process operation is simple, and the method is suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a degradable artificial dura mater with a double-layer structure and a preparation method thereof. Background technique [0002] The dura mater is an important tissue structure on the surface of the brain tissue, and it is an important barrier to protect the brain tissue. [0003] Open craniocerebral injury, inflammation, brain swelling, tumor invasion of meninges, excessive electrocautery and other factors may cause the dura mater to fail to close and form a defect. If it is not repaired in time or improperly repaired, it often causes complications such as cerebrospinal fluid leakage and intracranial infection. It is also worth noting that when the dura mater is missing outside the brain tissue, it is very easy to adhere to the surrounding tissue, and the surrounding new blood vessels will grow into the brain tissue to form a scar. Here it is the pathological basi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/58A61L27/22A61L27/24A61L27/56
CPCA61L27/58A61L27/24A61L27/227A61L27/56A61L2300/252A61L2300/418
Inventor 李亚婧黄文涛乔保坤
Owner 西安臻研生物科技有限公司
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