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High performance liquid chromatography method for detecting characteristic chromatograms of herbal materials, decoction pieces, standard decoction and formula granules of dried leaves of Nelumbo nucifera Gaertn.

A detection method and a technology of formula particles, applied in the field of HPLC detection, can solve the problems of different detection methods, difficult overall evaluation and control of the process, difficult delivery, etc., and achieve stable overall quality, high accuracy and good repeatability. Effect

Active Publication Date: 2018-11-30
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned reports are all based on the fingerprints of lotus leaf medicinal materials and formula granules, and the detection methods are different. Therefore, it is difficult to effectively compare and analyze the differences in the characteristic maps of lotus leaf medicinal materials, decoction pieces, standard decoctions, and formula granules. It is difficult to deeply understand the transfer of lotus leaf medicinal material basis in the technological process from medicinal materials to formula granules, and it is difficult to overall evaluate and control the technological process from lotus leaf medicinal materials to finished lotus leaf formula granules

Method used

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  • High performance liquid chromatography method for detecting characteristic chromatograms of herbal materials, decoction pieces, standard decoction and formula granules of dried leaves of Nelumbo nucifera Gaertn.
  • High performance liquid chromatography method for detecting characteristic chromatograms of herbal materials, decoction pieces, standard decoction and formula granules of dried leaves of Nelumbo nucifera Gaertn.
  • High performance liquid chromatography method for detecting characteristic chromatograms of herbal materials, decoction pieces, standard decoction and formula granules of dried leaves of Nelumbo nucifera Gaertn.

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 The detection method of the present invention is used for the detection of the HPLC characteristic spectrum of the lotus leaf medicinal material

[0044]1 Preparation of reference substance solution: take appropriate amount of reference substances of nuciferin, rutin, hyperin, and vetrin, and add methanol to prepare reference solutions containing 16, 200, 100, and 30 μg per 1 ml, respectively.

[0045] 2 Preparation of the reference medicinal material solution: take 1g of the lotus leaf reference medicinal material, accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 50ml of water, weigh it, decoct it for 45 minutes, let it cool, weigh it again, and make up the reduced amount with water. Lost weight, shake well, filter, that is.

[0046] 3 Preparation of the test solution: take 1g of the lotus leaf medicinal material to be tested, accurately weigh it, place it in a stoppered conical flask, add 50ml of water accurately, weigh it, deco...

Embodiment 2

[0051] Example 2 The detection method of the present invention is used for the detection of the HPLC characteristic spectrum of lotus leaf decoction pieces

[0052] 1 Preparation of reference substance solution: take appropriate amount of reference substances of nuciferin, rutin, hyperin, and vetrin, and add methanol to prepare reference solutions containing 16, 200, 100, and 30 μg per 1 ml, respectively.

[0053] 2 Preparation of the reference medicinal material solution: take 1g of the lotus leaf reference medicinal material, accurately weigh it, put it in a stoppered conical flask, accurately add 25ml of water, weigh it, decoct for 30 minutes, let it cool, weigh it again, and make up the reduced amount with water. Lost weight, shake well, filter, that is.

[0054] 3 Preparation of the test solution: take 1g of lotus leaf decoction pieces to be tested, accurately weigh, put in a stoppered Erlenmeyer bottle, add 25ml of water accurately, weigh, decoct for 30 minutes, let cool...

Embodiment 3

[0057] Embodiment 3 The detection method of the present invention is used for the detection of lotus leaf standard decoction HPLC characteristic spectrum

[0058] 1 Preparation of reference substance solution: take appropriate amount of reference substances of nuciferin, rutin, hyperin, and vetrin, and add methanol to prepare reference solutions containing 16, 200, 100, and 30 μg per 1 ml, respectively.

[0059] 2 Preparation of the reference medicinal material solution: take 1g of the lotus leaf reference medicinal material, accurately weigh it, put it in a stoppered conical flask, accurately add 25ml of water, weigh it, decoct for 30 minutes, let it cool, weigh it again, and make up the reduced amount with water. Lost weight, shake well, filter, that is.

[0060] 3 Preparation of the test solution: take 0.2g lotus leaf standard decoction to be tested, accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 10ml of water, weigh it, ultrasonically treat it ...

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Abstract

The invention provides a high performance liquid chromatography characteristic chromatogram detection method suitable for integrally controlling the quality of herbal materials, decoction pieces, a standard decoction and formula granules of dried leaves of Nelumbo nucifera Gaertn.. According to the HPLC specific chromatogram detection method, characteristic ingredients in the herbal materials, decoction pieces, standard decoction and formula granules of dried leaves of Nelumbo nucifera Gaertn. can be integrally controlled, and the stability of the integral quality of the herbal materials, decoction pieces, standard decoction and formula granules of dried leaves of Nelumbo nucifera Gaertn. can be guaranteed; and the method is easy to operate, high in precision, good in stability and repeatability and high in accuracy degree.

Description

technical field [0001] The invention specifically relates to an HPLC detection method for characteristic maps of lotus leaf medicinal materials, decoction pieces, standard decoctions and formula granules. Background technique [0002] The lotus leaf is the dried leaf of Nelumbo nucifera Gaertn., a plant of the water lily family. It was first recorded in "Diet Therapy Materia Medica". , Diarrhea due to spleen deficiency, spit mandrill due to blood heat, bloody stool and metrorrhagia, are commonly used clinical traditional Chinese medicine varieties. The lotus leaf mainly contains various alkaloid components such as nuciferine, protonuciferine, and pronuciferine, as well as flavonoids such as quercetin. Modern studies have shown that alkaloids in lotus leaves have pharmacological activities such as lipid-lowering, weight loss, antibacterial, and antiviral. The overall composition and content of these chemical components are the basis for the clinical efficacy of lotus leaf. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 周厚成胡昌江钟磊仰莲李文兵冯健
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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